An opto-structural method to estimate the stress-strain field induced by cell contraction on substrates of controlled stiffness in vitro

Journal of Applied Biomaterials and Functional Materials

Volumn 11, Issue 3, 2013, Pages 143-150

  Raimondi, Manuela Teresa ^a   Balconi, Giovanna ^b   Boschetti, Federica ^a   Di Metri, Antonio ^a   Azmi Mohammed, Salman Afroze ^b   Quaglini, Virginio ^a   Araneo, Lucio ^a   Galvéz, Beatriz G ^c   Lupi, Monica ^b   Latini, Roberto ^b   Remuzzi, Andrea ^d  

^a POLITECNICO DI MILANO   (Italy)

^b MARIO NEGRI INSTITUTE FOR PHARMACOLOGICAL RESEARCH   (Italy)

^c CENTRO NACIONAL DE INVESTIGACIONES CARDIOVASCULARES CNIC   (Spain)

^d UNIVERSITY OF BERGAMO   (Italy)

Author keywords

Contraction; Matrix; Mechanobiology; Mesoangioblast; Stem cell; Stiffness; Strain

Indexed keywords

BIOMECHANICS; CELL SIGNALING; CYTOLOGY; DEFORMATION; FLUORESCENCE; FLUORESCENCE MICROSCOPY; HYDROGELS; MICROSPHERES; STIFFNESS; STIFFNESS MATRIX; STRAIN; SUBSTRATES; TENSILE STRESS;

EXTRACELLULAR MATRICES; FIELD-INDUCED; IN-VITRO; MATRIX; MECHANO-BIOLOGY; MESOANGIOBLAST; STEM-CELL; STRESS-STRAIN FIELD; STRUCTURAL METHODS; SUBSTRATE DEFORMATION;

STEM CELLS;

AGAROSE; ALGINIC ACID;

ARTICLE; CALCULATION; CELL ADHESION; CELL CULTURE; CELL STRESS; EXTRACELLULAR MATRIX; GELATION; HYDROGEL; IN VITRO STUDY; MONOLAYER CULTURE; OPTO STRUCTURAL METHOD; PROCEDURES CONCERNING CELLS; RIGIDITY; SHEAR STRESS; STEM CELL; STRESS STRAIN FIELD; SURFACE PROPERTY; TENSILE STRENGTH; THICKNESS;

ALGINATES; ANIMALS; CELL ADHESION; CELL SHAPE; CELLS, CULTURED; ELASTIC MODULUS; EXTRACELLULAR MATRIX; GLUCURONIC ACID; HEXURONIC ACIDS; HYDROGELS; MICE; MICROSCOPY, FLUORESCENCE; SEPHAROSE; STRESS, MECHANICAL; TIME-LAPSE IMAGING;

EID: 84891543300     PISSN: None     EISSN: 22808000     Source Type: Journal    
DOI: 10.5301/JABFM.2012.9773     Document Type: Article

References (29)

1. Raimondi MT, Eaton SM, Laganà M, Aprile V, Nava MM, Cerullo G, Osellame R. Three-dimensional structural niches engineered via two-photon laser polymerization promote stem cell homing. Acta Biomater. 2012 Aug 21. pii: S1742-7061(12) 398-404. doi:10.1016/j.actbio.2012.08.022.
2. Arnsdorf EJ, Tummala P, Castillo AB, Zhang F, Jacobs CR. The epigenetic mechanism of mechanically induced osteogenic differentiation. J Biomech 2010; 43: 2881-6.
3. Huang AH, Farrell MJ, Mauck RL. Mechanics and mechanobiology of mesenchymal stem cell-based engineered cartilage. J Biomech 2010; 43: 128-36.
4. Huang Y, Jia X, Bai K, Gong X, Fan Y. Effect of fluid shear stress on cardiomyogenic differentiation of rat bone marrow mesenchymal stem cells. Arch Med Res 2010; 41: 497-505.
5. Kearney EM, Prendergast PJ, Campbell VA. Mechanisms of strain-mediated mesenchymal stem cell apoptosis. J Biomech Eng 2008; 130: 061004.
6. Smith IO, Liu XH, Smith LA, Ma PX. Nano-structured polymer scaffolds for tissue engineering and regenerative medicine. Wiley Interdiscip Rev Nanomed 2009; Nanobiotechnol 1: 226-236.
7. Nava MM, Raimondi MT, Pietrabissa R. Controlling selfrenewal and differentiation of stem cells via mechanical cues. J Biomed Biotechnol. 2012;2012:797410. doi:10.1155/2012/797410.
8. Raimondi MT, Eaton SM, Nava MM, Laganà M, Cerullo G, Osellame R. Two-photon laser polymerization: from fundamentals to biomedical application in tissue engineering and regenerative medicine. J Appl Biomater Function Mater. 2012 Jun 26;10(1):e56-66. doi:10.5301/JAB-FM.2012.9278.
9. Legant WR, Miller JS, Blakely BL, Cohen DM, Genin GM, Chen CS. Measurement of mechanical tractions exerted by cells in three-dimensional matrices. Nat Methods 2010; 7: 969-71.
10. Shivashankar GV. Mechanosignaling to Cell Nucleus and Gene Regulation. Annu Rev Biophys 2011; 40: 361-78.
11. Lee DA, Knight MM, Campbell JJ, Bader DL. Stem cell mechanobiology. J Cell Biochem 2011; 112: 1-9.
12. Dembo M and Wang YL. Stresses at the cell-to-substrate interface during locomotion of fibroblasts. Biophys J 1999; 76: 2307.
13. Butler JP, Tolic-Norrelykke IM, Fabry B, Fredberg JJ. Traction fields, moments, and strain energy that cells exert on their surroundings. Am J Physiol Cell Physiol 2002; 282: C595.
14. Yang Z, Lin JS, Chen J, Wang JHC. Determining substrate displacement and cell traction fields-a new approach. J Theor Biol 2006; 242: 607.
15. Tan JL, Tien J, Pirone DM, Gray DS, Bhadriraju K, Chen CS. Cells lying on a bed of microneedles: an approach to isolate mechanical force. Proc Nat Acad Sci U S A 2003; 100: 1484.
16. Beningo KA, Dembo M, Kaverina I, Small JV, Wang YL. Nascent focal adhesions are responsible for the generation of strong propulsive forces in migrating fibroblasts. J Cell Biol 2001; 153: 881.
17. Engler AJ, Carag-Krieger C, Johnson CP, et al. Embryonic cardiomyocytes beat best on a matrix with heart-like elasticity: scar-like rigidity inhibits beating. J Cell Sci 2008; 121: 3794-802.
18. Li B, Lin M, Tang Y, Wang B, Wang JH. A novel functional assessment of the differentiation of micropatterned muscle cells. J Biomech 2008; 41: 3349-53.
19. Fu J, Wang YK, Yang MT, et al. Mechanical regulation of cell function with geometrically modulated elastomeric substrates. Nat Methods 2010; 7: 733-6.
20. Galvez BG, Sampaolesi M, Barbuti A, et al. Cardiac mesoangioblasts are committed, self-renewable progenitors, associated with small vessels of juvenile mouse ventricle. Cell Death Differ 2008; 15: 1417-28.
21. Raffel M, Willert CE, Wereley ST, Kompenhans J. Particle image velocimetry, a practical guide. Springer, Berlin, Heidelberg. DOI:10.1007/978-3-540-72308-0. ISBN978-3-540-72307-3.
22. Quaglini V, Corazza C, Poggi C. Experimental characterization of orthotropic technical textiles under uniaxial and biaxial load. Composites: Part A Appl Sci Manuf 2008; 39: 1331-42.
23. Orive G, Hernández RM, Gascón AR, Igartua M, Pedraz JL. Survival of different cell lines in alginate-agarose microcapsules. Eur J Pharm Sci 2003; 18: 23-30.
24. Barlic A, Drobnic M, Malicev E, Kregar-Velikonja N. Quantitative analysis of gene expression in human articular chondrocytes assigned for autologous implantation. J Orthop Res 2008; 26: 847-53.
25. Ahearne M, Yang Y, El Haj AJ, Then KY, Liu KK. Characterizing the viscoelastic properties of thin hydrogel-based constructs for tissue engineering applications. J R Soc Interface 2005; 2: 455-63.
26. Cloyd JM, Malhotra NR, Weng L, Chen W, Mauck RL, Elliott DM. Material properties in unconfined compression of human nucleus pulposus, injectable hyaluronic acidbased hydrogels and tissue engineering scaffolds. Eur Spine J 2007; 16: 1892-8.
27. Hur S, Zhao Y, Li YS, Botvinick E, Chien S. Live Cells Exert 3-dimensional traction forces on their substrata. Cell Mol Bioeng 2009; 2: 425.
28. Delanoë-Ayari H, Ayari H, Rieu JP, Sano M. 4D Traction force microscopy reveals asymmetric cortical forces in migrating dictyostelium cells. Phys Rev Lett 2010; 105: 248103.
29. Maskarinec SA, Franck C, Tirrell DA, Ravichandran G. Quantifying cellular traction forces in three dimensions. Proc Nat Acad Sci U S A 2009; 106: 22108.