Tracking neuronal marker expression inside living differentiating cells using molecular beacons

Frontiers in Cellular Neuroscience

Volumn 7, Issue DEC, 2013, Pages

  Ilieva, Mirolyuba ^a   Vedova, Paolo Della ^a   Hansen, Ole ^a   Dufva, Martin ^a  

^a TECHNICAL UNIVERSITY OF DENMARK   (Denmark)

Author keywords

Differentiation; Gene expression; Molecular beacons; Neural stem cells; Neuronal marker

Indexed keywords

MESSENGER RNA; NESTIN; NEURON SPECIFIC NUCLEAR PROTEIN; OCTAMER TRANSCRIPTION FACTOR 4;

ARTICLE; CELL DIFFERENTIATION; CELL FUNCTION; CELL GROWTH; CELL HYBRIDIZATION; CELL PERMEABILIZATION; CELL VIABILITY; CONTROLLED STUDY; FLUORESCENCE; FLUORESCENCE MICROSCOPY; GENE EXPRESSION; GENETIC TRANSFECTION; HELA CELL; HUMAN; HUMAN CELL; IMAGE ANALYSIS; MATHEMATICAL MODEL; MOLECULAR BEACON; NUCLEOTIDE SEQUENCE; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SIMULATION;

EID: 84891119526     PISSN: 16625102     EISSN: None     Source Type: Journal    
DOI: 10.3389/fncel.2013.00266     Document Type: Article

References (26)

1. Bratu, D. P., Cha, B. J., Mhlanga, M. M., Kramer, F. R., and Tyagi, S. (2003). Visualizing the distribution and transport of mRNAs in living cells. Proc. Natl. Acad. Sci. U.S.A. 100, 13308-13313. doi: 10.1073/pnas.2233244100
2. Bustin, S. A. (2000). Absolute quantification of mRNA using real-time reverse tran-scription polymerase chain reaction assays. J. Mol. Endocrinol. 25, 169-193. doi: 10.1677/jme.0.0250169
3. Chen, A., Rhee, W. J., Bao, G., and Tsourkas, A. (2011). "Delivery of molecular beacons for live-cell imaging and analysis of RNA," in RNA Detection and Visualization. Methods and Protocols, Methods in Molecular Biology, Vol. 714, ed J. E. Gerst. (Humana press), 159-174. doi: 10.1007/978-1-61779-005-8_10
4. Chen A. K., Behlke, M. A., and Tsourkas, A. (2007). Avoiding false-positive signals with nuclease-vulnerable molecular beacons in single living cells. Nucleic Acids Res. 35, e105. doi: 10.1093/nar/gkm593
5. Desai, H. V., Voruganti, I. S., Jayasuriya, C., Chen, Q., and Darling, E. M. (2013). Live-cell, temporal gene expression analysis of osteogenic differentiation in adipose-derived stem cells. Tissue Eng. 19, 40-48. doi: 10.1089/ten.tea.2012.0127
6. Dobek, G. L., Zhang, X., Balazs, D. A., and Godbey, W. T. (2011). Analysis of promoters and expression-targeted gene therapy optimization based on doubling time and transfectability. FASEB J. 25, 3219-3228. doi: 10.1096/fj.11-185421
7. Ilieva, M., and Dufva, M. (2013). SOX2 and OCT4 mRNA-expressing cells, detected by molecular beacons, localize to the center of neurospheres during differentiation. PLoS ONE 8:e73669. doi: 10.1371/journal.pone.0073669
8. Kang, W. J., Cho, Y. L., Chae, J. R., Lee, J. D., Choi, K. J., and Kim, S. (2011). Molecular beacon based bioimaging of multiple microRNAs during myoge-nesis. Biomaterials 32, 1915-1922. doi: 10.1016/j.biomaterials.2010.11.007
9. Karra, D., and Dahm, R. (2010). Transfection techniques for neuronal cells. J. Neurosci. 30, 6171-6177. doi: 10.1523/JNEUROSCI.0183-10.2010
10. Larsson, H. M., Lee, S. T., Roccio, M., Velluto, D., Lutolf, M. P., Frey, P., et al. (2012). Sorting live stem cells based on Sox 2 mRNA expression. PLoS ONE 7:e49874. doi: 10.1371/journal.pone.0049874
11. Lee, J. Y., Colinas, J., Wang, J. Y., Mace, D., Ohler, U., and Benfey, P. (2006). Transcriptional and posttranscriptional regulation of transcription factor expression in Arabidopsis roots. Proc. Natl. Acad. Sci. U.S.A. 103, 6055-6060. doi: 10.1073/pnas.0510607103
12. Lennon, F. E., Hermann, C. D., Olivares-Navarrete, R., Rhee, W. J., Schwartz, Z., Bao, G., et al. (2010). Use of molecular beacons to image effects of titanium surface microstructure on β1 integrin expression in live osteoblast-like cells. Biomaterials 31, 7640-7647. doi: 10.1016/j.biomaterials.2010.07.009
13. Lotharius, J., Barg, S., Wiekop, P., Lundberg, C., Raymon, H. K., and Brundin, P. (2002). Effect of mutant α-synuclein on dopamine homeostasis in a new human mesencephalic cell line. J. Biol. Chem. 277, 38884-38894. doi: 10.1074/jbc.M205518200
14. Lotharius, J., Falsig, J., van Beek, J., Payne, S., Dringen, R., Brundin, P., et al. (2005). Progressive degeneration of human mesencephalic neuronderived cells triggered by dopamine-dependent oxidative stress is dependent on the mixed-lineage kinase pathway. J. Neurosci. 25, 6329-6342. doi: 10.1523/JNEUROSCI.1746-05.2005
15. Niedermeyer, W. (1985). Interaction of streptolysin-O with biomembranes: kinetic and morphological studies on erythrocyte membranes. Toxicon 23, 425-439. doi: 10.1016/0041-0101(85)90026-1
16. Palmer, M., et al. (1998). Streptolysin O: a proposed model of allosteric interaction between a pore-forming protein and its target lipid bilayer. Biochemistry 37, 2378-2383. doi: 10.1021/bi9720890
17. Ramos-Moreno, T., Lendínez, J. G., Pino-Barrio, M. J., Arco, A., and MartínezSerrano, A. (2012). Clonal human fetal ventral mesencephalic dopaminergic neuron precursors for cell therapy research. PLoS ONE 7:e52714. doi: 10.1371/journal.pone.0052714
18. Rhee, W. J., and Bao, G. (2009). Simultaneous detection of mRNA and protein stem cell markers in live cells. BMC Biotechnol. 9:30. doi: 10.1186/1472-6750-9-30
19. Rhee, W. J., and Bao, G. (2010). Slow non-specific accumulation of 2′-deoxy and 2′-O-methyl oligonucleotide probes at mitochondria in live cells. Nucleic Acids Res. 38, e109. doi: 10.1093/nar/gkq050
20. Rhee, W. J., Santangelo, P. J., Jo, H., and Bao, G. (2008). Target accessibility and signal specificity in live-cell detection of BMP-4 mRNA using molecular beacons. Nucleic Acids Res. 36, e30. doi: 10.1093/nar/gkn039
21. SantaLucia, J. Jr. (1998). A unified view of polymer, dumbbell, and oligonucleotide DNA nearest-neighbor thermodynamics. Proc. Natl. Acad. Sci. U.S.A. 95, 1460-1465. doi: 10.1073/pnas.95.4.1460
22. Santangelo, P. J., Nix, B., Tsourkas, A., and Bao, G. (2004). Dual FRET molecular beacons for mRNA detection in living cells. Nucleic Acids Res. 32, e57. doi: 10.1093/nar/gnh062
23. Scholz, D., Pöltl, D., Genewsky, A., Weng, M., Waldmann, T., Shildknecht, S., et al. (2011). Rapid, complete and large-scale generation of post-mitotic neurons from the human LUHMES cell line. J. Neurochem. 119, 957-971. doi: 10.1111/j.1471-4159.2011.07255.x
24. Tsourkas, A., Behlke, M. A., and Bao, G. (2002). Hybridization of 2′-O-methyl and 2′-deoxy molecular beacons to RNA and DNA targets. Nucleic Acids Res. 30, 5168-5174. doi: 10.1093/nar/gkf635
25. Tyagi, S., and Kramer, F. R. (1996). Molecular beacons: probes that fluoresce upon hybridization. Nat. Biotechnol. 14, 303-308. doi: 10.1038/nbt0396-303
26. Zhao, J., Wang, Z. Q., Wang, X. Y., Yang, X. J., and He, D. (2010). Preliminary study of diagnostic utility of molecular beacons in bladder cancer. Urology 76, 512.e8-512.e13. doi: 10.1016/j.urology.2010.04.023