Puromycin resistance gene as an effective selection marker for ciliate Tetrahymena

Gene

Volumn 534, Issue 2, 2014, Pages 249-255

  Iwamoto, Masaaki ^a   Mori, Chie ^a   Hiraoka, Yasushi ^a,b   Haraguchi, Tokuko ^a,b  

^a NATIONAL INSTITUTE OF INFORMATION AND COMMUNICATIONS TECHNOLOGY   (Japan)

^b OSAKA UNIVERSITY   (Japan)

Author keywords

Ciliate; Gene manipulation; Puromycin N acetyltransferase; Selection marker; Tetrahymena thermophila; Transformation

Indexed keywords

PUROMYCIN; PUROMYCIN N ACETYLTRANSFERASE; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ARTICLE; CELL GROWTH; CELL TRANSFORMATION; CILIATE; CODON USAGE; DNA SEQUENCE; GENE LOCUS; GENETIC TRANSFECTION; GROWTH CURVE; HOMOLOGOUS RECOMBINATION; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; TETRAHYMENA THERMOPHILA;

CILIOPHORA; EUKARYOTA; TETRAHYMENA; TETRAHYMENA THERMOPHILA;

3′ UTR; 4-(2-HYDROXYETHYL)-1-PIPERAZINEETHANESULFONIC ACID; 6-METHYLPURINE SENSITIVE; 90% INHIBITORY CONCENTRATION; BETA-TUBULIN GENE; BLASTICIDIN S-RESISTANT GENE; BSR; BTU2; CHX1-1; CILIATE; CY-S; CYCLOHEXIMIDE SENSITIVE; GENE MANIPULATION; GENE MUTATION EXPRESSING 6-METHYLPURINE SENSITIVE; GENE MUTATION EXPRESSING CYCLOHEXIMIDE SENSITIVE; HEPES; HHF1; HHO1; HISTONE H1 GENE; HISTONE H4 GENE; IC90; METALLOTHIONEIN GENE; MP-S; MPR1-1; MTT1; NEO; NEOMYCIN-RESISTANT GENE; NUCLEOPORIN SEH1 GENE; PAC; PLASMID VECTOR FOR THE EXPRESSION OF GFP FUSION PROTEIN IN VEGETATIVE TETRAHYMENA CELLS; PUROMYCIN-N-ACETYLTRANSFERASE; PUROMYCIN-N-ACETYLTRANSFERASE GENE; PVGF1; SEH1; SELECTION MARKER; TETRAHYMENA THERMOPHILA; THREE PRIME UNTRANSLATED REGION; TRANSFORMATION;

ACETYLTRANSFERASES; AMINO ACID SEQUENCE; BASE SEQUENCE; BIOLOGICAL MARKERS; CELLS, CULTURED; CILIOPHORA; CODON; DRUG RESISTANCE; GENE EXPRESSION; MOLECULAR SEQUENCE DATA; PUROMYCIN; TETRAHYMENA THERMOPHILA;

EID: 84890433555     PISSN: 03781119     EISSN: 18790038     Source Type: Journal    
DOI: 10.1016/j.gene.2013.10.049     Document Type: Article

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