Neomycin and puromycin affect gene expression in Giardia lamblia stable transfection

Molecular and Biochemical Parasitology

Volumn 156, Issue 2, 2007, Pages 124-135

  Su, Li Hsin ^a   Lee, Gilbert A ^a   Huang, Yu Chang ^a   Chen, Yi Hsiu ^a   Sun, Chin Hung ^a  

^a NATIONAL TAIWAN UNIVERSITY   (Taiwan)

Author keywords

Giardia; Neomycin; Puromycin; Transfection

Indexed keywords

CARBAMATE KINASE; CHAPERONE; CYCLOPHILIN; CYST WALL PROTEIN 1; CYST WALL PROTEIN 2; GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; NEOMYCIN; ORNITHINE CARBAMOYLTRANSFERASE; PHOSPHOGLYCERATE KINASE; PROTEIN; PROTEIN MYB; PROTEIN MYB2; PROTEIN P21; PUROMYCIN; UNCLASSIFIED DRUG;

ARTICLE; CELL ASSAY; CELL DIFFERENTIATION; CELL LINE; CONTROLLED STUDY; GENE; GENE DISRUPTION; GENE EXPRESSION; GENE EXPRESSION REGULATION; GENETIC TRANSCRIPTION; GENETIC TRANSFECTION; GIARDIA LAMBLIA; NONHUMAN; NORTHERN BLOTTING; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN FOLDING; PROTEIN METABOLISM; PROTEOMICS; TRANSCRIPTION INITIATION SITE; UPREGULATION;

ANIMALS; BLOTTING, NORTHERN; ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; GENE EXPRESSION; GENE EXPRESSION REGULATION; GIARDIA LAMBLIA; NEOMYCIN; PROTEOME; PROTOZOAN PROTEINS; PUROMYCIN; RNA, PROTOZOAN; TRANSFECTION; UP-REGULATION;

GIARDIA; GIARDIA INTESTINALIS;

EID: 35348969738     PISSN: 01666851     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.molbiopara.2007.07.015     Document Type: Article

References (55)

1. Adam R.D. Biology of Giardia lamblia. Clin Microbiol Rev 14 (2001) 447-475
2. Hashimoto T., Nakamura Y., Nakamura F., et al. Protein phylogeny gives a robust estimation for early divergences of eukaryotes: phylogenetic place of a mitochondria-lacking protozoan, Giardia lamblia. Mol Biol Evol 11 (1994) 65-71
3. Hashimoto T., Nakamura Y., Kamaishi T., et al. Phylogenetic place of mitochondrion-lacking protozoan, Giardia lamblia, inferred from amino acid sequences of elongation factor 2. Mol Biol Evol 12 (1995) 782-793
4. Sogin M.L., Gunderson J.H., Elwood H.J., Alonso R.A., and Peattie D.A. Phylogenetic meaning of the kingdom concept: an unusual ribosomal RNA from Giardia lamblia. Science 243 (1989) 75-77
5. Eichinger D. Encystation in parasitic protozoa. Curr Opin Microbiol 4 (2001) 421-426
6. Gillin F.D., and Reiner D.S. Cell biology of the primitive eukaryote Giardia lamblia. Annu Rev Microbiol 50 (1996) 679-705
7. Knodler L.A., Svard S.G., Silberman J.D., Davids B.J., and Gillin F.D. Developmental gene regulation in Giardia lamblia: first evidence for an encystation-specific promoter and differential 5′ mRNA processing. Mol Microbiol 34 (1999) 327-340
8. Lujan H.D., Mowatt M.R., Conrad J.T., Bowers B., and Nash T.E. Identification of a novel Giardia lamblia cyst wall protein with leucine-rich repeats. Implications for secretory granule formation and protein assembly into the cyst wall. J Biol Chem 270 (1995) 29307-29313
9. Mowatt M.R., Lujan H.D., Cotton D.B., et al. Developmentally regulated expression of a Giardia lamblia cyst wall protein gene. Mol Microbiol 15 (1995) 955-963
10. Sun C.H., McCaffery J.M., Reiner D.S., and Gillin F.D. Mining the Giardia lamblia genome for new cyst wall proteins. J Biol Chem 278 (2003) 21701-21708
11. Sun C.H., Palm D., McArthur A.G., Svard S.G., and Gillin F.D. A novel Myb-related protein involved in transcriptional activation of encystation genes in Giardia lamblia. Mol Microbiol 46 (2002) 971-984
12. Hou G., Le Blancq S.M.E.Y., Zhu H., and Lee M.G. Structure of a frequently rearranged rRNA-encoding chromosome in Giardia lamblia. Nucl Acids Res 23 (1995) 3310-3317
13. Bernander R., Palm J.E., and Svard S.G. Genome ploidy in different stages of the Giardia lamblia life cycle. Cell Microbiol 1 (2001) 55-62
14. Sun C.H., Chou C.F., and Tai J.H. Stable DNA transfection of the primitive protozoan pathogen Giardia lamblia. Mol Biochem Parasitol 92 (1998) 123-132
15. Sun C.H., and Tai J.H. Development of a tetracycline controlled gene expression system in the parasitic protozoan Giardia lamblia. Mol Biochem Parasitol 105 (2000) 51-60
16. Singer S.M., Yee J., and Nash T.E. Episomal and integrated maintenance of foreign DNA in Giardia lamblia. Mol Biochem Parasitol 92 (1998) 59-69
17. Yu D.C., Wang A.L., and Wang C.C. Stable coexpression of a drug-resistance gene and a heterologous gene in an ancient parasitic protozoan Giardia lamblia. Mol Biochem Parasitol 83 (1996) 81-91
18. Dan M., Wang A.L., and Wang C.C. Inhibition of pyruvate-ferredoxin oxidoreductase gene expression in Giardia lamblia by a virus-mediated hammerhead ribozyme. Mol Microbiol 36 (2000) 447-456
19. Touz M.C., Kulakova L., and Nash T.E. Adaptor protein complex 1 mediates the transport of lysosomal proteins from a Golgi-like organelle to peripheral vacuoles in the primitive eukaryote Giardia lamblia. Mol Biol Cell 15 (2004) 3053-3060
20. Eustice D.C., and Wilhelm J.M. Fidelity of the eukaryotic codon-anticodon interaction: interference by aminoglycoside antibiotics. Biochemistry 23 (1984) 1462-1467
21. Schroeder R., Waldsich C., and Wank H. Modulation of RNA function by aminoglycoside antibiotics. EMBO J 19 (2000) 1-9
22. Burke J.F., and Mogg A.E. Suppression of a nonsense mutation in mammalian cells in vivo by the aminoglycoside antibiotics G-418 and paromomycin. Nucl Acids Res 13 (1985) 6265-6272
23. Nathans D. Puromycin inhibition of protein synthesis: incorporation of puromycin into peptide chains. Proc Natl Acad Sci USA 51 (1964) 585-592
24. Kirillov S., Porse B.T., Vester B., Woolley P., and Garrett R.A. Movement of the 3′-end of tRNA through the peptidyl transferase centre and its inhibition by antibiotics. FEBS Lett 406 (1997) 223-233
25. Bevilacqua M.A., Faniello M.C., Russo T., Cimino F., and Costanzo F. Transcriptional regulation of the human H ferritin-encoding gene (FERH) in G418-treated cells: role of the B-box-binding factor. Gene 141 (1994) 287-291
26. Graham S.V., and Barry J.D. Polysomal, procyclin mRNAs accumulate in bloodstream forms of monomorphic and pleomorphic trypanosomes treated with protein synthesis inhibitors. Mol Biochem Parasitol 80 (1996) 179-191
27. Purdy J.E., Donelson J.E., and Wilson M.E. Regulation of genes encoding the major surface protease of Leishmania chagasi via mRNA stability. Mol Biochem Parasitol 142 (2005) 88-97
28. Shetty S. Protein synthesis and urokinase mRNA metabolism. Mol Cell Biochem 271 (2005) 13-22
29. Koeller D.M., Horowitz J.A., Casey J.L., Klausner R.D., and Harford J.B. Translation and the stability of mRNAs encoding the transferrin receptor and c-fos. Proc Natl Acad Sci USA 88 (1991) 7778-7782
30. Lim S.K., Sigmund C.D., Gross K.W., and Maquat L.E. Nonsense codons in human beta-globin mRNA result in the production of mRNA degradation products. Mol Cell Biol 12 (1992) 1149-1161
31. Keister D.B. Axenic culture of Giardia lamblia in TYI-S-33 medium supplemented with bile. Trans R Soc Trop Med Hyg 77 (1983) 487-488
32. Gillin F.D., Boucher S.E., Rossi S.S., and Reiner D.S. Giardia lamblia: the roles of bile, lactic acid, and pH in the completion of the life cycle in vitro. Exp Parasitol 69 (1996) 164-174
33. McArthur A.G., Morrison H.G., Nixon J.E., et al. The Giardia genome project database. FEMS Microbiol Lett 189 (2000) 271-273
34. Li Lei, and Wang C.C. A likely molecular basis of the susceptibility of Giardia lamblia towards oxygen. Mol Microbiol 59 (2006) 202-211
35. Yee J., Mowatt M.R., Dennis P.P., and Nash T.E. Transcriptional analysis of the glutamate dehydrogenase gene in the primitive eukaryote, Giardia lamblia. Identification of a primordial gene promoter. J Biol Chem 275 (2000) 11432-11439
36. Sun C.H., Su L.H., and Gillin F.D. Novel plant-GARP-like transcription factors in Giardia lamblia. Mol Biochem Parasitol 146 (2006) 45-57
37. Sun C.H., and Tai J.H. Identification and characterization of a ran gene promoter in the primitive protozoan pathogen Giardia lamblia. J Biol Chem 274 (1999) 19699-19706
38. Bjellqvist B., Sanchez J.C., Pasquali C., et al. Micropreparative two-dimensional electrophoresis allowing the separation of samples containing milligram amounts of proteins. Electrophoresis 14 (1993) 1375-1378
39. Shevchenko A., Jensen O.N., Podtelejnikov A.V., et al. Linking genome and proteome by mass spectrometry: large-scale identification of yeast proteins from two dimensional gels. Proc Natl Acad Sci USA 93 (1996) 14440-14445
40. Sun C.H., Su L.H., and Gillin F.D. Influence of 5′ sequences on expression of the Tet repressor in Giardia lamblia. Mol Biochem Parasitol 142 (2005) 1-11
41. Palm D., Weiland M., McArthur A.G., et al. Developmental changes in the adhesive disk during Giardia differentiation. Mol Biochem Parasitol 141 (2005) 199-207
42. Wang C.H., Su L.H., and Sun C.H. A novel ARID/Bright-like protein involved in transcriptional activation of cyst wall protein 1 gene in Giardia lamblia. J Biol Chem 282 (2007) 8905-8914
43. Freeman B.C., Toft D.O., and Morimoto R. Molecular chaperone machines: chaperone activities of the cyclophilin Cyp-40 and the steroid aporeceptor-associated protein p23. Science 247 (1996) 1718-1720
44. Jin Z.G., Melaragno M.G., Liao D.F., et al. Cyclophilin A is a secreted growth factor induced by oxidative stress. Circ Res 87 (2000) 789-796
45. Winteringham L.N., Kobelke S., Williams J.H., Ingley E., and Klinken S.P. Myeloid leukemia factor 1 inhibits erythropoietin-induced differentiation, cell cycle exit and p27Kip1 accumulation. Oncogene 23 (2004) 5105-5109
46. Bose S., Weikl T., Bugl H., and Buchner J. Chaperone function of Hsp90-associated proteins. Science 247 (1996) 1715-1717
47. Stefanic S., Palm D., Svard S.G., and Hehl A.B. Organelle proteomics reveals cargo maturation mechanisms associated with Golgi-like encystation vesicles in the early-diverged protozoan Giardia lamblia. J Biol Chem 281 (2006) 7595-7604
48. McArthur A.G., Knodler L.A., Silberman J.D., Davids B.J., Gillin F.D., and Sogin M.L. The evolutionary origins of eukaryotic protein disulfide isomerase domains: new evidence from the Amitochondriate protist Giardia lamblia. Mol Biol Evol 18 (2001) 1455-1463
49. Kaufman R.J. Stress signaling from the lumen of the endoplasmic reticulum: coordination of gene transcriptional and translational controls. Genes Dev 13 (1999) 1211-1233
50. Yoshida H., Matsui T., Hosokawa N., Kaufman R.J., Nagata K., and Mori K. A time-dependent phase shift in the mammalian unfolded protein response. Dev cell 4 (2003) 265-271
51. Davis-Hayman S.R., Hayman J.R., and Nash T.E. Encystation-specific regulation of the cyst wall protein 2 gene in Giardia lamblia by multiple cis-acting elements. Int J Parasitol 33 (2003) 1005-1012
52. Webb H., Burns R., Ellis L., Kimblin N., and Carrington M. Developmentally regulated instability of the GPI-PLC mRNA is dependent on a short-lived protein factor. Nucl Acids Res 33 (2005) 1503-1512
53. Anderson P., and Kedersha N. RNA granules. J Cell Biol 172 (2006) 803-808
54. Simoni M., and Gromoll J. Monitoring the transfection efficiency of the human follicle-stimulating hormone receptor cDNA in COS-7 cells: evaluation of the growth hormone transient gene expression assay system. J Endocrinol Invest 19 (1996) 359-364
55. Flint A., Kaluz S., Kaluzova M., Sheldrick L., Fisher P., and Derecka K. Co-transfected SV40 origin of replication activates expression from SV40 promoterless constructs. Biotechniques 27 (1999) 728-732