Transformation and Strain Engineering of Tetrahymena

Methods in Cell Biology

Volumn 109, Issue , 2012, Pages 327-345

  Chalker, Douglas L ^a  

^a WASHINGTON UNIVERSITY   (United States)

Author keywords

Bombardment; Electroporation; Homozygous; Mutant; Recombination; Transformation

Indexed keywords

EID: 84858737375     PISSN: 0091679X     EISSN: None     Source Type: Book Series    
DOI: 10.1016/B978-0-12-385967-9.00011-6     Document Type: Chapter

References (41)

1. Allen S.L. Cytogenetics of genomic exclusion in Tetrahymena. Genetics 1967, 55:797-822.
2. Bright L.J., Kambesis N., Nelson S.B., Jeong B., Turkewitz A.P. Comprehensive analysis reveals dynamic and evolutionary plasticity of Rab GTPases and membrane traffic in Tetrahymena thermophila. PLoS Genet 2010, 6(10):e1001155.
3. Bruns P.J., Brussard T.B. Pair formation in Tetrahymena pyriformis, an inducible developmental system. J. Exp. Zool. 1974, 188:337-344.
4. Busch C.J., Vogt A., Mochizuki K. Establishment of a Cre/loxP recombination system for N-terminal epitope tagging of genes in Tetrahymena. BMC Microbiol. 2010, 10:191.
5. Cassidy-Hanley D., Bowen J., Lee J.H., Cole E., VerPlank L.A., Gaertig J., Gorovsky M.A., Bruns P.J. Germline and somatic transformation of mating Tetrahymena thermophila by particle bombardment. Genetics 1997, 146:135-147.
6. Cervantes M.D., Coyne R.S., Xi X., Yao M.C. The condensin complex is essential for amitotic segregation of bulk chromosomes, but not nucleoli, in the ciliate Tetrahymena thermophila. Mol. Cell Biol. 2006, 26:4690-4700.
7. Chalker D.L., Ward J.G., Randolph C., Yao M.-C. Microinjection of Tetrahymena thermophila. Tetrahymena thermophila 1999, 469-484. Academic Press, New York. D.J. Asai, J.D. Forney (Eds.).
8. Chilcoat N.D., Elde N.C., Turkewitz A.P. An antisense approach to phenotype-based gene cloning in Tetrahymena. Proc. Natl. Acad. Sci. U.S.A. 2001, 98:8709-8713.
9. Fass J., Joshi N., Couvillion M., Bowen J., Gorovsky M., Hamilton E., Orias E., Hong K., Coyne R., Eisen J., Chalker D., Lin D., Collins K. Genome-scale analysis of programmed DNA elimination sites in Tetrahymena thermophila. G3: Genes, Genomes, and Genetics 2011, 1:515-522.
10. Gaertig J., Gorovsky M.A. Efficient mass transformation of Tetrahymena thermophila by electroporation of conjugants. Proc. Natl. Acad. Sci. U.S.A. 1992, 89:9196-9200.
11. Gaertig J., Gorovsky M.A. DNA-mediated transformation in Tetrahymena. Methods Cell Biol. 1995 a, 47:559-569.
12. Gaertig J., Gorovsky M.A. Gene transfer by electroporation in Tetrahymena. Methods Mol. Biol. 1995 b, 47:331-348.
13. Gaertig J., Gu L., Hai B., Gorovsky M.A. High frequency vector-mediated transformation and gene replacement in Tetrahymena. Nucleic Acids Res. 1994 a, 22:5391-5398.
14. Gaertig J., Kapler G. Transient and stable DNA transformation of Tetrahymena thermophila by electroporation. Methods Cell Biol. 2000, 62:485-500.
15. Gaertig J., Thatcher T.H., Gu L., Gorovsky M.A. Electroporation-mediated replacement of a positively and negatively selectable beta-tubulin gene in Tetrahymena thermophila. Proc. Natl. Acad. Sci. U.S.A. 1994 b, 91:4549-4553.
16. Godiska R., Yao M.C. A programmed site-specific DNA rearrangement in Tetrahymena thermophila requires flanking polypurine tracts. Cell 1990, 61:1237-1246.
17. Hartley J.L., Temple G.F., Brasch M.A. DNA cloning using in vitro site-specific recombination. Genome Res. 2000, 10:1788-1795.
18. Iwamoto M., Mori C., Kojidani T., Bunai F., Hori T., Fukagawa T., Hiraoka Y., Haraguchi T. Two distinct repeat sequences of Nup98 nucleoporins characterize dual nuclei in the binucleated ciliate tetrahymena. Curr. Biol. 2009, 19:843-847.
19. Kataoka K., Schoeberl U.E., Mochizuki K. Modules for C-terminal epitope tagging of Tetrahymena genes. J. Microbiol. Methods 2010, 82:342-346.
20. Kowalczyk C.A., Anderson A.M., Arce-Larreta M., Chalker D.L. The germ line limited M element of Tetrahymena is targeted for elimination from the somatic genome by a homology-dependent mechanism. Nucleic Acids Res. 2006, 34(20):5778-5789.
21. Larson D.D., Blackburn E.H., Yeager P.C., Orias E. Control of rDNA replication in Tetrahymena involves a cis-acting upstream repeat of a promoter element. Cell 1986, 47:229-240.
22. Linger B.R., Morin G.B., Price C.M. The Pot1A-associated proteins TPT1 and PAT1 coordinate telomere protection and length regulation in Tetrahymena. Mol. Biol. Cell. 2011, 22(21):4161-4170.
23. Malone C.D., Falkowska K.A., Li A.Y., Galanti S.E., Kanuru R.C., LaMont E.G., Mazzarella K.C., Micev A.J., Osman M.M., Piotrowski N.K., Suszko J.W., Timm A.C., Xu M.M., Liu L., Chalker D.L. Nucleus-specific importin alpha proteins and nucleoporins regulate protein import and nuclear division in the binucleate Tetrahymena thermophila. Eukaryot. Cell 2008, 7:1487-1499.
24. Martindale D.W., Allis C.D., Bruns P. Conjugation in Tetrahymena thermophila: a temporal analysis of cytological stages. Exp. Cell Res. 1982, 140:227-236.
25. Mochizuki K. High efficiency transformation of Tetrahymena using a codon-optimized neomycin resistance gene. Gene 2008, 425(1-2):79-83.
26. Orias E., Larson D., Hu Y.F., Yu G.L., Karttunen J., Lovlie A., Haller B., Blackburn E.H. Replacement of the macronuclear ribosomal RNA genes of a mutant Tetrahymena using electroporation. Gene 1988, 70:295-301.
27. Shang Y., Song X., Bowen J., Corstanje R., Gao Y., Gaertig J., Gorovsky M.A. A robust inducible-repressible promoter greatly facilitates gene knockouts, conditional expression, and overexpression of homologous and heterologous genes in Tetrahymena thermophila. Proc. Natl. Acad. Sci. U.S.A. 2002, 99:3734-3739.
28. Spangler E.A., Blackburn E.H. The nucleotide sequence of the 17S ribosomal RNA gene of Tetrahymena thermophila and the identification of point mutations resulting in resistance to the antibiotics paromomycin and hygromycin. J. Biol. Chem. 1985, 260:6334-6340.
29. Tondravi M.M., Yao M.C. Transformation of Tetrahymena thermophila by microinjection of ribosomal RNA genes. Proc. Natl. Acad. Sci. U.S.A. 1986, 83:4369-4373.
30. Walhout A.J., Temple G.F., Brasch M.A., Hartley J.L., Lorson M.A., van den Heuvel S., Vidal M. GATEWAY recombinational cloning: application to the cloning of large numbers of open reading frames or ORFeomes. Methods Enzymol 2000, 328:575-592.
31. Wenkert D., Allis C.D. Timing of the appearance of macronuclear-specific histone variant hv1 and gene expression in developing new macronuclei of Tetrahymena thermophila. J. Cell Biol. 1984, 98:2107-2117.
32. Witkin K.L., Collins K. Holoenzyme proteins required for the physiological assembly and activity of telomerase. Genes Dev. 2004, 18:1107-1118.
33. Yao M.C., Blackburn E., Gall J.G. Amplification of the rRNA genes in Tetrahymena. Cold Spring Harb. Symp. Quant. Biol. 1979, 2:1293-1296.
34. Yao M.C., Gall J.G. A single integrated gene for ribosomal RNA in a eucaryote. Tetrahymena pyriformis. Cell 1977, 12:121-132.
35. Yao M.C., Yao C.H. Accurate processing and amplification of cloned germ line copies of ribosomal DNA injected into developing nuclei of Tetrahymena thermophila. Mol. Cell Biol. 1989, 9:1092-1099.
36. Yao M.C., Yao C.H. Transformation of Tetrahymena to cycloheximide resistance with a ribosomal protein gene through sequence replacement. Proc. Natl. Acad. Sci. U.S.A. 1991, 88:9493-9497.
37. Yao M.C., Yao C.H., Halasz L.M., Fuller P., Rexer C.H., Wang S.H., Jain R., Coyne R.S., Chalker D.L. Identification of novel chromatin-associated proteins involved in programmed genome rearrangements in Tetrahymena. J. Cell Sci. 2007, 120:1978-1989.
38. Yao M.C., Zhu S.G., Yao C.H. Gene amplification in Tetrahymena thermophila: formation of extrachromosomal palindromic genes coding for rRNA. Mol. Cell Biol. 1985, 5:1260-1267.
39. Yasuda L.F., Yao M.C. Short inverted repeats at a free end signal large palindromic DNA formation in Tetrahymena. Cell 1991, 67:505-516.
40. Yu G.L., Blackburn E.H. Transformation of Tetrahymena thermophila with a mutated circular ribosomal DNA plasmid vector. Proc. Natl. Acad. Sci. U.S.A. 1989, 86:8487-8491.
41. Yu G.L., Hasson M., Blackburn E.H. Circular ribosomal DNA plasmids transform Tetrahymena thermophila by homologous recombination with endogenous macronuclear ribosomal DNA. Proc. Natl. Acad. Sci. U.S.A. 1988, 85:5151-5155.