Analysis of SOS-induced spontaneous prophage induction in Corynebacterium glutamicum at the single-cell level

Journal of Bacteriology

Volumn 196, Issue 1, 2014, Pages 180-188

  Nanda, Arun M ^a   Heyer, Antonia ^a   Krämer, Christina ^a   Grünberger, Alexander ^a   Kohlheyer, Dietrich ^a   Frunzke, Julia ^a  

^a FORSCHUNGSZENTRUM JÜLICH   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; BACTERIAL GENE; BACTERIAL GROWTH; BACTERIAL STRAIN; BACTERIAL VIABILITY; CONTROLLED STUDY; CORYNEBACTERIUM GLUTAMICUM; DNA CROSS LINKING; DNA DAMAGE; DNA REPAIR; FLOW CYTOMETRY; GENE EXPRESSION; GROWTH CURVE; PRIORITY JOURNAL; PROPHAGE; REPORTER GENE; STRESS;

ARTIFICIAL GENE FUSION; CORYNEBACTERIUM GLUTAMICUM; DNA DAMAGE; GENES, REPORTER; LUMINESCENT PROTEINS; LYSOGENY; PROMOTER REGIONS, GENETIC; SOS RESPONSE (GENETICS); VIRUS ACTIVATION;

EID: 84890381347     PISSN: 00219193     EISSN: 10985530     Source Type: Journal    
DOI: 10.1128/JB.01018-13     Document Type: Article

References (38)

1. Casjens S. 2003. Prophages and bacterial genomics: what have we learned so far? Mol. Microbiol. 49:277-300. http://dx.doi.org/10.1046/j.1365-2958.2003.03580.x.
2. Ajinomoto Co I. 2011. Food products business. http://www.ajinomoto.com/en/ir/pdf/Feed-useAA-Oct2011.pdf. Ajinomoto Co., Inc., Tokyo, Japan.
3. Ajinomoto Co I. 2012. Feed-use amino acids business. http://www.ajinomoto.com/en/ir/pdf/Food-Oct2012.pdf. Ajinomoto Co., Inc., Tokyo, Japan.
4. Kalinowski J. 2005. The genomes of amino acid-producing corynebacteria, p 37-56. In Eggeling L, Bott M (ed), Handbook of Corynebacterium glutamicum. CRC Press, Boca Raton, FL.
5. Frunzke J, Bramkamp M, Schweitzer J-E, Bott M. 2008. Population Heterogeneity in Corynebacterium glutamicum ATCC 13032 caused by prophage CGP3. J. Bacteriol. 190:5111-5119. http://dx.doi.org/10.1128/JB.00310-08.
6. Baumgart M, Unthan S, Rückert C, Sivalingam J, Grünberger A, Kalinowski J, Bott M, Noack S, Frunzke J. 2013. Construction of a prophagefree variant of Corynebacterium glutamicum ATCC 13032-a platform strain for basic research and industrial biotechnology. Appl. Environ. Microbiol. 79:6006-6015. http://dx.doi.org/10.1128/AEM.01634-13.
7. Gödeke J, Paul K, Lassak J, Thormann KM. 2011. Phage-induced lysis enhances biofilm formation in Shewanella oneidensis MR-1. ISME J. 5:613-626. http://dx.doi.org/10.1038/ismej.2010.153.
8. Carrolo M, Frias MJ, Pinto FR, Melo-Cristino J, Ramirez M. 2010. Prophage spontaneous activation promotes DNA release enhancing biofilm formation in Streptococcus pneumoniae, PLoS One 5:e15678. http://dx.doi.org/10.1371/journal.pone.0015678.
9. Flemming H-C, Neu TR, Wozniak DJ. 2007. The EPS matrix: the "house of biofilm cells." J. Bacteriol. 189:7945-7947. http://dx.doi.org/10.1128/JB.00858-07.
10. Whitchurch CB, Tolker-Nielsen T, Ragas PC, Mattick JS. 2002. Extracellular DNA required for bacterial biofilm formation. Science 295:1487. http://dx.doi.org/10.1126/science.295.5559.1487.
11. LwoffA. 1953. Lysogeny. Bacteriol. Rev. 17:269-332.
12. Luria. 1967. Transduction studies on the role of a rec gene in the ultraviolet induction of prophage lambda. J. Mol. Biol. 23:117-133. http://dx.doi.org/10.1016/S0022-2836(67)80021-4.
13. Oppenheim AB, Kobiler O, Stavans J, Court DL, Adhya S. 2005. Switches in bacteriophage lambda development. Annu. Rev. Genet. 39: 409-429. http://dx.doi.org/10.1146/annurev.genet.39.073003.113656.
14. Friedberg EC, Walker GC, Siede W, Wood RD, Schultz RA, Ellenberger T. 2005. DNA repair and mutagenesis. ASM Press, Washington, DC.
15. Grünberger A, Paczia N, Probst C, Schendzielorz G, Eggeling L, Noack S, Wiechert W, Kohlheyer D. 2012. A disposable picolitre bioreactor for cultivationandinvestigation of industrially relevant bacteriaonthe single cell level. Lab. Chip. 12:2060-2068. http://dx.doi.org/10.1039/c2lc40156h.
16. MustafiN, Grünberger A, Kohlheyer D, Bott M, Frunzke J. 2012. The development and application of a single-cell biosensor for the detection of L-methionine and branched-chain amino acids. Metab. Eng. 14:449-457. http://dx.doi.org/10.1016/j.ymben.2012.02.002.
17. Keilhauer C, Eggeling L, Sahm H. 1993. Isoleucine synthesis in Corynebacterium glutamicum: molecular analysis of the ilvB-ilvN-ilvC operon. J. Bacteriol. 175:5595-5603.
18. Kensy F, Zang E, Faulhammer C, Tan R-K, Büchs J. 2009. Validation of a high-throughput fermentation system based on online monitoring of biomass and fluorescence in continuously shaken microtiter plates. Microb. Cell Fact. 8:31. http://dx.doi.org/10.1186/1475-2859-8-31.
19. Inoue H, Nojima H, Okayama H. 1990. High efficiency transformation of Escherichia coli with plasmids. Gene 96:23-28. http://dx.doi.org/10.1016/0378-1119(90)90336-P.
20. Van der Rest ME, Lange C, Molenaar D. 1999. A heat shock following electroporation induces highly efficient transformation of Corynebacterium glutamicum with xenogeneic plasmid DNA. Appl. Microbiol. Biotechnol. 52:541-545. http://dx.doi.org/10.1007/s002530051557.
21. Eikmanns B, Thum-Schmitz N. 1994. Nucleotide sequence, expression and transcriptional analysis of the Corynebacterium glutamicum gltA gene encoding citrate synthase. Microbiology 140:1817-1828. http://dx.doi.org/10.1099/13500872-140-8-1817.
22. Jochmann N, Kurze AK, Czaja LF, Brinkrolf K, Brune I, Hüser AT, Hansmeier N, Pühler A, Borovok I, Tauch A. 2009. Genetic makeup of the Corynebacterium glutamicum LexA regulon deduced from comparative transcriptomics and in vitro DNA band shift assays. Microbiology 155:1459-1477. http://dx.doi.org/10.1099/mic.0.025841-0.
23. Hentschel E, Will C, MustafiN, Burkovski A, Rehm N, Frunzke J. 2013. Destabilized eYFP variants for dynamic gene expression studies in Corynebacterium glutamicum, Microb. Biotechnol. 6:196-201. http://dx.doi.org/10.1111/j.1751-7915.2012.00360.x.
24. Strack RL, Hein B, Bhattacharyya D, Hell SW, Keenan RJ, Glick BS. 2009. A rapidly maturing far-red derivative of DsRed-Express2 for wholecell labeling. Biochemistry 48:8279-8281. http://dx.doi.org/10.1021/bi900870u.
25. Grünberger A, Probst C, Heyer A, Wiechert W, Frunzke J, Kohlheyer D. Microfluidic picoliter bioreactor for microbial single cell analysis: fabrication, system setup and operation. J. Vis Exp., in press.
26. Ogino H, Teramoto H, Inui M, Yukawa H. 2008. DivS, a novel SOSinducible cell-division suppressor in Corynebacterium glutamicum, Mol. Microbiol. 67:597-608. http://dx.doi.org/10.1111/j.1365-2958.2007.06069.x.
27. Pennington JM, Rosenberg SM. 2007. Spontaneous DNA breakage in single living Escherichia coli cells. Nat. Genet. 39:797-802. http://dx.doi.org/10.1038/ng2051.
28. Kamenšek S, Podlesek Z, Gillor O, Zgur-Bertok D. 2010. Genes regulated by the Escherichia coli SOS repressor LexA exhibit heterogeneous expression. BMC Microbiol. 10:283. http://dx.doi.org/10.1186/1471-2180-10-283.
29. Bailone A, Levine A, Devoret R. 1979. Inactivation of prophage repressor in vivo. J. Mol. Biol. 131:553-572. http://dx.doi.org/10.1016/0022-2836(79)90007-X.
30. Cox MM, Goodman MF, Kreuzer KN, Sherratt DJ, Sandlerk SJ, Marians KJ. 2000. The importance of repairing stalled replication forks. Nature 2:37-41.
31. Rothstein R, Michel B, GangloffS. 2000. Replication fork pausing and recombination or "gimme a break." Gene Dev. 14:1-10.
32. Bossi L, Fuentes J, Mora G, Figueroa-Bossi N. 2003. Prophage contribution to bacterial population dynamics. J. Bacteriol. 185:6467-6471. http://dx.doi.org/10.1128/JB.185.21.6467-6471.2003.
33. Makarova KS, Wolf YI, Koonin EV. 2009. Comprehensive comparativegenomic analysis of type 2 toxin-antitoxin systems and related mobile stress response systems in prokaryotes. Biol. Direct 4:19. http://dx.doi.org/10.1186/1745-6150-4-19.
34. Looe JM, Looe M, Wêgrzyn A, Wêgrzyn G. 2012. Altruism of Shiga toxin-producing Escherichia coli: recent hypothesis versus experimental results. Front. Cell Infect. Microbiol. 2:166. http://dx.doi.org/10.3389/fcimb.2012.00166.
35. Watve M, Parab S, Jogdand P, Keni S. 2006. Aging may be a conditional strategic choice and not an inevitable outcome for bacteria. Proc. Natl. Acad. Sci. U. S. A. 103:14831-14835. http://dx.doi.org/10.1073/pnas.0606499103.
36. Kinoshita S, Udaka S, Shimono M. 2004. Studies on the amino acid fermentation. Part 1. Production of L-glutamic acid by various microorganisms. J. Gen. Appl. Microbiol. 50:331-343. http://dx.doi.org/10.2323/jgam.3.193.
37. Schäfer A, Tauch A, Jäger Kalinowski WJ, Thierbach G, Pühler A. 1994. Small mobilizable multi-purpose cloning vectors derived from the Escherichia coli plasmids pK18 and pK19: selection of defined deletions in the chromosome of Corynebacterium glutamicum, Gene 145:69-73. http://dx.doi.org/10.1016/0378-1119(94)90324-7.
38. Cremer J, Eggeling L, Sahm H. 1991. Control of the lysine biosynthesis sequence in Corynebacterium glutamicum as analyzed by overexpression of the individual corresponding genes. Appl. Environ. Microbiol. 57: 1746-1752.w