Development and validation of MRM methods to quantify protein isoforms of polyphenol oxidase in loquat fruits

Journal of Proteome Research

Volumn 12, Issue 12, 2013, Pages 5709-5722

  Martínez Márquez, Ascensión ^a   Morante Carriel, Jaime ^a,b   Sellés Marchart, Susana ^a   Martínez Esteso, María José ^a   Pineda Lucas, José Luis ^d   Luque, Ignacio ^c   Bru Martínez, Roque ^a  

^a UNIVERSITY OF ALICANTE   (Spain)

^b UNIVERSIDAD TÉCNICA ESTATAL DE QUEVEDO   (Ecuador)

^c UNIVERSITY OF SEVILLE   (Spain)

^d Poligono Industrial Oeste Cl Principal   (Spain)

Author keywords

isoform; loquat fruits; multiple reaction monitoring; polyphenol oxidase; transitions

Indexed keywords

CATECHOL OXIDASE; ISOENZYME; PEPTIDE FRAGMENT; RECOMBINANT PROTEIN; VEGETABLE PROTEIN;

ACCURACY; AMINO ACID SEQUENCE; ANALYTIC METHOD; ARTICLE; ENZYME ACTIVITY; ENZYME ASSAY; FRUIT; ION TRAP MASS SPECTROMETRY; LOQUAT; MASS SPECTROMETER; MOLECULAR CLONING; MOLECULAR WEIGHT; MULTIPLE REACTION MONITORING; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN DETERMINATION; PROTEIN EXPRESSION; QUADRUPOLE MASS SPECTROMETRY; QUANTITATIVE ANALYSIS; REPRODUCIBILITY; SENSITIVITY AND SPECIFICITY; SEQUENCE ALIGNMENT; TRIPLE QUADRUPOLE MASS SPECTROMETRY; VALIDITY; CALIBRATION; CHEMISTRY; ENZYMOLOGY; ERIOBOTRYA; GENE EXPRESSION REGULATION; GENETICS; ISOLATION AND PURIFICATION; LIQUID CHROMATOGRAPHY; METABOLISM; MOLECULAR GENETICS; MULTIGENE FAMILY; PROTEIN DEGRADATION; SEQUENCE HOMOLOGY; TANDEM MASS SPECTROMETRY;

AMINO ACID SEQUENCE; CALIBRATION; CATECHOL OXIDASE; CHROMATOGRAPHY, LIQUID; CLONING, MOLECULAR; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ERIOBOTRYA; FRUIT; GENE EXPRESSION REGULATION, PLANT; ISOENZYMES; MOLECULAR SEQUENCE DATA; MULTIGENE FAMILY; PEPTIDE FRAGMENTS; PLANT PROTEINS; PROTEOLYSIS; RECOMBINANT PROTEINS; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY, AMINO ACID; TANDEM MASS SPECTROMETRY;

EID: 84890065180     PISSN: 15353893     EISSN: 15353907     Source Type: Journal    
DOI: 10.1021/pr4006712     Document Type: Article

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