EHMT1 controls brown adipose cell fate and thermogenesis through the PRDM16 complex

Nature

Volumn 504, Issue 7478, 2013, Pages 163-167

  Ohno, Haruya ^a   Shinoda, Kosaku ^a   Ohyama, Kana ^a   Sharp, Louis Z ^a   Kajimura, Shingo ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

EUCHROMATIC HISTONE LYSINE N METHYLTRANSFERASE 1; HISTONE H3; HISTONE LYSINE METHYLTRANSFERASE; LYSINE; PRDM16 PROTEIN; TRANSCRIPTION FACTOR; UNCLASSIFIED DRUG;

CYTOLOGY; ENZYME ACTIVITY; FAT; GENE EXPRESSION; HOMEOSTASIS; MOLECULAR ANALYSIS; OBESITY;

ADIPOCYTE; ANIMAL CELL; ARTICLE; BROWN ADIPOSE TISSUE; CELL FATE; COMPLEX FORMATION; CONTROLLED STUDY; DEMETHYLATION; ENERGY METABOLISM; GENE DELETION; IN VIVO STUDY; INSULIN RESISTANCE; MALE; MOUSE; MUSCLE DEVELOPMENT; NONHUMAN; OBESITY; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN EXPRESSION; THERMOGENESIS; THERMOREGULATION;

ADIPOSE TISSUE, BROWN; ANIMALS; CELL DIFFERENTIATION; CELLS, CULTURED; DNA-BINDING PROTEINS; ENERGY METABOLISM; HEK293 CELLS; HISTONE-LYSINE N-METHYLTRANSFERASE; HUMANS; MICE; THERMOGENESIS; TRANSCRIPTION FACTORS;

EID: 84889604511     PISSN: 00280836     EISSN: 14764687     Source Type: Journal    
DOI: 10.1038/nature12652     Document Type: Article

References (36)

1. Seale, P. et al. PRDM16 controls a brown fat/skeletal muscle switch. Nature 454, 961-967 (2008).
2. Kajimura, S. et al. Initiation ofmyoblast to brownfatswitch by a PRDM16-C/EBP-b transcriptional complex. Nature 460, 1154-1158 (2009).
3. Nedergaard, J., Bengtsson, T. & Cannon, B. Unexpected evidence for active brown adipose tissue in adult humans. Am. J. Physiol. 293, E444-E452 (2007).
4. van Marken Lichtenbelt, W. D. et al. Cold-activated brown adipose tissue in healthy men. N. Engl. J. Med. 360, 1500-1508 (2009).
5. Saito, M. et al. High incidence of metabolically active brown adipose tissue in healthy adult humans: effects of cold exposure and adiposity. Diabetes 58, 1526-1531 (2009).
6. Virtanen, K. A. et al. Functional brown adipose tissue in healthy adults. N. Engl. J. Med. 360, 1518-1525 (2009).
7. Atit, R. et al. b-catenin activation is necessary and sufficient to specify the dorsal dermal fate in the mouse. Dev. Biol. 296, 164-176 (2006).
8. Timmons, J. A. et al. Myogenic gene expression signature establishes that brown and white adipocytes originate fromdistinct cell lineages. Proc. Natl Acad. Sci. USA 104, 4401-4406 (2007).
9. Kajimura, S., Seale, P. & Spiegelman, B. M. Transcriptional control of brown fat development. Cell Metab. 11, 257-262 (2010).
10. Shing, D. C. et al. Overexpression of sPRDM16 coupled with loss of p53 induces myeloid leukemias in mice. J. Clin. Invest. 117, 3696-3707 (2007).
11. Pinheiro, I. et al. Prdm3 and Prdm16 are H3K9me1 methyltransferases required for mammalian heterochromatin integrity. Cell 150, 948-960 (2012).
12. Tachibana, M. et al. Histone methyltransferases G9a and GLP form heteromeric complexes and are both crucial for methylation of euchromatin at H3-K9. Genes Dev. 19, 815-826 (2005).
13. Kleefstra, T. et al. Disruption of the gene euchromatin histone methyl transferase1 (Eu-HMTase1) is associated with the 9q34 subtelomeric deletion syndrome. J. Med. Genet. 42, 299-306 (2005).
14. Cormier-Daire, V. et al. Cryptic terminal deletion of chromosome 9q34: a novel cause of syndromic obesity in childhood? J. Med. Genet. 40, 300-303 (2003).
15. Willemsen, M. H. et al. Update on Kleefstra syndrome. Mol. Syndromol. 2, 202-212 (2012).
16. Kajimura, S. et al. Regulation of the brown and white fat gene programs through a PRDM16/CtBP transcriptional complex. Genes Dev. 22, 1397-1409 (2008).
17. Schaefer, A. et al. Control of cognition and adaptive behavior by the GLP/G9a epigenetic suppressor complex. Neuron 64, 678-691 (2009).
18. Tachibana, M., Matsumura, Y., Fukuda, M., Kimura, H. & Shinkai, Y. G9a/GLP complexes independently mediate H3K9 and DNA methylation to silence transcription. EMBO J. 27, 2681-2690 (2008).
19. Eguchi, J. et al. Transcriptional control ofadipose lipid handling by IRF4.CellMetab. 13, 249-259 (2011).
20. Almind, K., Manieri, M., Sivitz, W. I., Cinti, S. & Kahn, C. R. Ectopic brown adipose tissue in muscle provides a mechanism for differences in risk of metabolic syndrome in mice. Proc. Natl Acad. Sci. USA 104, 2366-2371 (2007).
21. Cannon, B. & Nedergaard, J. Nonshivering thermogenesis and its adequate measurement in metabolic studies. J. Exp. Biol. 214, 242-253 (2011).
22. Ouellet, V. et al. Outdoor temperature, age, sex, body mass index, and diabetic status determine the prevalence, mass, and glucose-uptake activity of 18F-FDGdetected BAT in humans. J. Clin. Endocrinol. Metab. 96, 192-199 (2012).
23. Wu, Q. et al. Fatty acid transport protein 1 is required for nonshivering thermogenesis in brown adipose tissue. Diabetes 55, 3229-3237 (2006).
24. Feldmann, H. M., Golozoubova, V., Cannon, B. & Nedergaard, J. UCP1 ablation induces obesity and abolishes diet-induced thermogenesis in mice exempt from thermal stress by living at thermoneutrality. Cell Metab. 9, 203-209 (2009).
25. Yoneshiro, T. et al. Impact of UCP1 and beta3AR gene polymorphisms on agerelated changes in brown adipose tissue and adiposity in humans. Int. J. Obes. 37, 993-998 (2013).
26. Huh, M. S., Parker, M. H., Scime, A., Parks, R. & Rudnicki, M. A. Rb is required for progression through myogenic differentiation but not maintenance of terminal differentiation. J. Cell Biol. 166, 865-876 (2004).
27. Mao, X. et al. APPL1 binds to adiponectin receptors and mediates adiponectin signalling and function. Nature Cell Biol. 8, 516-523 (2006).
28. Liisberg Aune, U., Ruiz, L. & Kajimura, S. Isolation and differentiation of stromal vascular cells to beige/brite cells. J Visual. Exp. e50191 (2013).
29. Takahashi, A. et al. DNA damage signaling triggers degradation of histone methyltransferases through APC/C(Cdh1) in senescent cells. Mol. Cell 45, 123-131 (2012).
30. Kurn, N. et al. Novel isothermal, linear nucleic acidamplification systemsfor highly multiplexed applications. Clin. Chem. 51, 1973-1981 (2005).
31. Trapnell, C. et al. Differential analysis of gene regulation at transcript resolution with RNA-seq. Nature Biotechnol. 31, 46-53 (2013).
32. Huang, W., Sherman, B. T. & Lempicki, R. A. Systematic and integrative analysis of large gene lists using DAVID bioinformatics resources. Nature Protocols 4, 44-57 (2009).
33. Kersey, P. J. et al. The International Protein Index: an integrated database for proteomics experiments. Proteomics 4, 1985-1988 (2004).
34. Elias, J. E. & Gygi, S. P. Target-decoy search strategy for increased confidence in large-scale protein identifications by mass spectrometry. Nature Methods 4, 207-214 (2007).
35. Seale, P. et al. Transcriptional control of brown fat determination by PRDM16. Cell Metab. 6, 38-54 (2007).
36. Ohno, H., Shinoda, K., Spiegelman, B. M. & Kajimura, S. PPARc agonists induce a white-to-brown fat conversion through stabilization of PRDM16 protein. Cell Metab. 15, 395-404 (2012).