Reduced PMA enhances the responsiveness of transfected THP-1 macrophages to polarizing stimuli

Journal of Immunological Methods

Volumn 402, Issue 1-2, 2014, Pages 76-81

  Maeß, Marten B ^a   Wittig, Berith ^a   Cignarella, Andrea ^b   Lorkowski, Stefan ^a  

^a FRIEDRICH SCHILLER UNIVERSITY JENA   (Germany)

^b UNIVERSITY OF PADOVA   (Italy)

Author keywords

Activation; IL10; Polarization; SiRNA; THP 1 macrophages; Transfection

Indexed keywords

EID: 84889264781     PISSN: 00221759     EISSN: 18727905     Source Type: Journal    
DOI: 10.1016/j.jim.2013.11.006     Document Type: Article

References (22)

1. Adachi S., Kuwata T., Miyaike M., Iwata M. Induction of CCR7 expression in thymocytes requires both ERK signal and Ca(2+) signal. Biochem. Biophys. Res. Commun. 2001, 288:1188.
2. Ansa-Addo E.A., Lange S., Stratton D., Antwi-Baffour S., Cestari I., Ramirez M.I., McCrossan M.V., Inal J.M. Human plasma membrane-derived vesicles halt proliferation and induce differentiation of THP-1 acute monocytic leukemia cells. J. Immunol. 2010, 185:5236.
3. Auwerx J. The human leukemia cell line, THP-1: a multifaceted model for the study of monocyte-macrophage differentiation. Experientia 1991, 47:22.
4. Buers I., Robenek H., Lorkowski S., Nitschke Y., Severs N.J., Hofnagel O. TIP47, a lipid cargo protein involved in macrophage triglyceride metabolism. Arterioscler. Thromb. Vasc. Biol. 2009, 29:767.
5. Chang Z.L. Recent development of the mononuclear phagocyte system: in memory of Metchnikoff and Ehrlich on the 100th Anniversary of the 1908 Nobel Prize in Physiology or Medicine. Biol. Cell. 2009, 101:709.
6. Dumrese C., Slomianka L., Ziegler U., Choi S.S., Kalia A., Fulurija A., Lu W., Berg D.E., Benghezal M., Marshall B., Mittl P.R. The secreted Helicobacter cysteine-rich protein A causes adherence of human monocytes and differentiation into a macrophage-like phenotype. FEBS Lett. 2009, 583:1637.
7. Guh J.H., Chang W.L., Yang J., Lee S.L., Wei S., Wang D., Kulp S.K., Chen C.S. Development of novel adenosine monophosphate-activated protein kinase activators. J. Med. Chem. 2010, 53:2552.
8. Joshi A.D., Oak S.R., Hartigan A.J., Finn W.G., Kunkel S.L., Duffy K.E., Das A., Hogaboam C.M. Interleukin-33 contributes to both M1 and M2 chemokine marker expression in human macrophages. BMC Immunol. 2010, 11:52.
9. Lang R., Patel D., Morris J.J., Rutschman R.L., Murray P.J. Shaping gene expression in activated and resting primary macrophages by IL-10. J. Immunol. 2002, 169:2253.
10. Lee W.Y., Chin A.C., Voss S., Parkos C.A. In vitro neutrophil transepithelial migration. Methods Mol. Biol. 2006, 341:205.
11. Liu W.S., Heckman C.A. The sevenfold way of PKC regulation. Cell. Signal. 1998, 10:529.
12. Ma W., Liu Y., Ellison N., Shen J. Induction of C-X-C chemokine receptor type 7 (CXCR7) switches stromal cell-derived factor-1 (SDF-1) signaling and phagocytic activity in macrophages linked to atherosclerosis. J. Biol. Chem. 2013, 288:15481.
13. Maeß M.B., Sendelbach S., Lorkowski S. Selection of reliable reference genes during THP-1 monocyte differentiation into macrophages. BMC Mol. Biol. 2010, 11:90.
14. Maeß M.B., Buers I., Robenek H., Lorkowski S. Improved protocol for efficient nonviral transfection of premature THP-1 macrophages. Cold Spring Harb. Protoc. 2011, 2011.
15. Maeß M.B., Stolle K., Cullen P., Lorkowski S. Evidence for an alternative genomic structure, mRNA and protein sequence of human ABCA13. Gene 2013, 515:298.
16. Mosser D.M. The many faces of macrophage activation. J. Leukoc. Biol. 2003, 73:209.
17. Mosser D.M., Edwards J.P. Exploring the full spectrum of macrophage activation. Nat. Rev. Immunol. 2008, 8:958.
18. Park E.K., Jung H.S., Yang H.I., Yoo M.C., Kim C., Kim K.S. Optimized THP-1 differentiation is required for the detection of responses to weak stimuli. Inflamm. Res. 2007, 56:45.
19. Ricardo S.D., van Goor H., Eddy A.A. Macrophage diversity in renal injury and repair. J. Clin. Invest. 2008, 118:3522.
20. Schnoor M., Buers I., Sietmann A., Brodde M.F., Hofnagel O., Robenek H., Lorkowski S. Efficient non-viral transfection of THP-1 cells. J. Immunol. Methods 2009, 344:109.
21. Stolle K., Schnoor M., Fuellen G., Spitzer M., Cullen P., Lorkowski S. Cloning, genomic organization, and tissue-specific expression of the RASL11B gene. Biochim. Biophys. Acta 2007, 1769:514.
22. Tsuchiya S., Kobayashi Y., Goto Y., Okumura H., Nakae S., Konno T., Tada K. Induction of maturation in cultured human monocytic leukemia cells by a phorbol diester. Cancer Res. 1982, 42:1530.