Employment of colorimetric enzyme assay for monitoring expression and solubility of GST fusion proteins targeted to inclusion bodies

Journal of Biotechnology

Volumn 168, Issue 4, 2013, Pages 506-510

  Mačinković, Igor S ^a,b   Abughren, Mohamed ^a   Mrkic, Ivan ^a   Grozdanović, Milica M ^a   Prodanović, Radivoje ^a   Gavrović Jankulović, Marija ^a  

^a UNIVERSITY OF BELGRADE   (Serbia)

^b UNIVERSITY OF ERLANGEN NUREMBERG   (Germany)

Author keywords

GST; GST tagged proteins; Inclusion bodies; Urea

Indexed keywords

1-CHLORO-2 , 4-DINITROBENZENE; BIOACTIVE PROTEINS; DENATURING CONDITIONS; GST; INCLUSION BODIES; PROTEIN EXPRESSIONS; QUANTITATIVE MEASUREMENT; RECOMBINANT PROTEIN EXPRESSION;

ASSAYS; COLORIMETRY; ESCHERICHIA COLI; METABOLISM; SOLUBILITY; UREA;

PROTEINS;

1 CHLORO 2,4 DINITROBENZENE; HYBRID PROTEIN; UREA;

ARTICLE; CELL INCLUSION; COLORIMETRY; CONTROLLED STUDY; DERMATOPHAGOIDES; ENZYME ASSAY; ENZYME INACTIVATION; HUMAN; PLACENTA; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN TARGETING; SOLUBILIZATION;

GST; GST-TAGGED PROTEINS; INCLUSION BODIES; UREA;

ALLERGENS; ANIMALS; COLORIMETRY; ESCHERICHIA COLI; GENE EXPRESSION REGULATION, BACTERIAL; GLUTATHIONE TRANSFERASE; INCLUSION BODIES; MICE; RECOMBINANT FUSION PROTEINS; UREA;

EID: 84888826710     PISSN: 01681656     EISSN: 18734863     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2013.09.019     Document Type: Article

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