Efficient expansion of mesenchymal stem cells from mouse bone marrow under hypoxic conditions

Journal of Tissue Engineering and Regenerative Medicine

Volumn 7, Issue 12, 2013, Pages 984-993

  Yew, Tu Lai ^a   Chang, Ming Chau ^a,b   Hsu, Yuan Tong ^b   He, Fan Yu ^b   Weng, Wen Hui ^c   Tsai, Chih Chien ^a,b   Chiu, Fang Yao ^b   Hung, Shih Chieh ^a,b  

^a NATIONAL YANG MING UNIVERSITY   (Taiwan)

^b TAIPEI VETERANS GENERAL HOSPITAL   (Taiwan)

^c NATIONAL TAIPEI UNIVERSITY OF TECHNOLOGY   (Taiwan)

Author keywords

Hypoxic culture; Low density culture; Mesenchymal stem cells; Mouse

Indexed keywords

FLOWCHARTING; MAMMALS; STEM CELLS;

BONE MARROW; HIGH QUALITY; HYPOXIC CONDITION; HYPOXIC CULTURE; LOW DENSITY CULTURE; LOWER DENSITY; MESENCHYMAL STEM CELL; MOUSE; MOUSE BONES; THERAPEUTIC POTENTIALS;

CELL CULTURE;

ATAXIN 1; BETA1 INTEGRIN; CD11B ANTIGEN; CD31 ANTIGEN; CD34 ANTIGEN; CD45 ANTIGEN; ENDOGLIN; HERMES ANTIGEN;

ADHERENT CELL; ADIPOCYTE; ANIMAL CELL; ARTICLE; BONE MARROW CELL; CARTILAGE CELL; CELL DIFFERENTIATION; CELL ISOLATION; CONTROLLED STUDY; DENSITY; FLOW CYTOMETRY; HYPOXIA; MALE; MESENCHYMAL STEM CELL; MOUSE; NONHUMAN; OSTEOBLAST; PRIORITY JOURNAL; STEM CELL CULTURE; STEM CELL EXPANSION;

HYPOXIC CULTURE; LOW DENSITY CULTURE; MESENCHYMAL STEM CELLS; MOUSE;

ANIMALS; BONE MARROW CELLS; CELL CULTURE TECHNIQUES; CELL DIFFERENTIATION; CELL HYPOXIA; CELL MEMBRANE; CELL PROLIFERATION; CELLS, CULTURED; GENE EXPRESSION REGULATION; KARYOTYPING; MESENCHYMAL STROMAL CELLS; MICE;

EID: 84888434457     PISSN: 19326254     EISSN: 19327005     Source Type: Journal    
DOI: 10.1002/term.1491     Document Type: Article

References (39)

1. Aguayo-Mazzucato C, Bonner-Weir S. 2010; Stem cell therapy for type 1 diabetes mellitus. Nat Rev Endocrinol 6: 139-148.
2. Baddoo M, Hill K, Wilkinson R, et al. 2003; Characterization of mesenchymal stem cells isolated from murine bone marrow by negative selection. J Cell Biochem 89: 1235-1249.
3. Baksh D, Song L, Tuan RS. 2004, Adult mesenchymal stem cells: characterization, differentiation, and application in cell and gene therapy. J Cell Mol Med 8: 301-316.
4. Busuttil RA, Rubio M, Dolle ME, et al. 2003; Oxygen accelerates the accumulation of mutations during the senescence and immortalization of murine cells in culture. Aging Cell 2: 287-294.
5. Castro-Malaspina H, Gay RE, Resnick G, et al. 1980; Characterization of human bone marrow fibroblast colony-forming cells (CFU-F) and their progeny. Blood 56: 289-301.
6. Falla N, Van V, Bierkens J, et al. 1993; Characterization of a 5-fluorouracil-enriched osteoprogenitor population of the murine bone marrow. Blood 82: 3580-3591.
7. Fan G, Wen L, Li M, et al. 2011; Isolation of mouse mesenchymal stem cells with normal ploidy from bone marrows by reducing oxidative stress in combination with extracellular matrix. BMC Cell Biol 12: 30.
8. Friedenstein AJ, Chailakhjan RK, Lalykina KS. 1970; The development of fibroblast colonies in monolayer cultures of guinea-pig bone marrow and spleen cells. Cell Tissue Kinet 3: 393-403.
9. Goldberg MA, Gaut CC, Bunn HF. 1991; Erythropoietin mRNA levels are governed by both the rate of gene transcription and posttranscriptional events. Blood 77: 271-277.
10. Grinnemo KH, Mansson A, Dellgren G, et al. 2004; Xenoreactivity and engraftment of human mesenchymal stem cells transplanted into infarcted rat myocardium. J Thorac Cardiovasc Surg 127: 1293-1300.
11. Horwitz EM, Gordon PL, Koo WK, et al. 2002; Isolated allogeneic bone marrow-derived mesenchymal cells engraft and stimulate growth in children with osteogenesis imperfecta: implications for cell therapy of bone. Proc Natl Acad Sci USA 99: 8932-8937.
12. Hung SC, Chen NJ, Hsieh SL, et al. 2002a; Isolation and characterization of size-sieved stem cells from human bone marrow. Stem Cells 20: 249-258.
13. Hung SC, Cheng H, Pan CY, et al. 2002b; In vitro differentiation of size-sieved stem cells into electrically active neural cells. Stem Cells 20: 522-529.
14. Hung SC, Pochampally RR, Chen SC, et al. 2007a; Angiogenic effects of human multipotent stromal cell conditioned medium activate the PI3K-Akt pathway in hypoxic endothelial cells to inhibit apoptosis, increase survival, and stimulate angiogenesis. Stem Cells 25: 2363-2370.
15. Hung SC, Pochampally RR, Hsu SC, et al. 2007b; Short-term exposure of multipotent stromal cells to low oxygen increases their expression of CX3CR1 and CXCR4 and their engraftment in vivo. PLoS One 2: e416.
16. Hung SC, Yang DM, Chang CF, et al. 2004; Immortalization without neoplastic transformation of human mesenchymal stem cells by transduction with HPV16 E6/E7 genes. Int J Cancer 110: 313-319.
17. Johansson AC, Visse E, Widegren B, et al. 2001; Computerized image analysis as a tool to quantify infiltrating leukocytes: a comparison between high- and low-magnification images. J Histochem Cytochem 49: 1073-1079.
18. Koc ON, Gerson SL, Cooper BW, et al. 2000; Rapid hematopoietic recovery after coinfusion of autologous-blood stem cells and culture-expanded marrow mesenchymal stem cells in advanced breast cancer patients receiving high-dose chemotherapy. J Clin Oncol 18: 307-316.
19. Le Blanc K, Rasmusson I, Sundberg B, et al. 2004; Treatment of severe acute graft-versus-host disease with third party haploidentical mesenchymal stem cells. Lancet 363: 1439-1441.
20. Modderman WE, Vrijheid-Lammers T, Lowik CW, et al. 1994; Removal of hematopoietic cells and macrophages from mouse bone marrow cultures: isolation of fibroblastlike stromal cells. Exp Hematol 22: 194-201.
21. Nadri S, Soleimani M. 2007; Isolation murine mesenchymal stem cells by positive selection. In Vitro Cell Dev Biol Anim 43: 276-282.
22. Peister A, Mellad JA, Larson BL, et al. 2004; Adult stem cells from bone marrow (MSCs) isolated from different strains of inbred mice vary in surface epitopes, rates of proliferation, and differentiation potential. Blood 103: 1662-1668.
23. Petite H, Viateau V, Bensaid W, et al. 2000; Tissue-engineered bone regeneration. Nat Biotechnol 18: 959-963.
24. Phinney DG, Kopen G, Isaacson RL, et al. 1999; Plastic adherent stromal cells from the bone marrow of commonly used strains of inbred mice: variations in yield, growth, and differentiation. J Cell Biochem 72: 570-585.
25. Pittenger MF, Mackay AM, Beck SC, et al. 1999; Multilineage potential of adult human mesenchymal stem cells. Science 284: 143-147.
26. Prockop DJ. 1997; Marrow stromal cells as stem cells for nonhematopoietic tissues. Science 276: 71-74.
27. Quarto R, Mastrogiacomo M, Cancedda R, et al. 2001; Repair of large bone defects with the use of autologous bone marrow stromal cells. N Engl J Med 344: 385-386.
28. Sekiya I, Larson BL, Smith JR, et al. 2002; Expansion of human adult stem cells from bone marrow stroma: conditions that maximize the yields of early progenitors and evaluate their quality. Stem Cells 20: 530-541.
29. Short BJ, Brouard N, Simmons PJ. 2009; Prospective isolation of mesenchymal stem cells from mouse compact bone. Methods Mol Biol 482: 259-268.
30. Soleimani M, Nadri S. 2009; A protocol for isolation and culture of mesenchymal stem cells from mouse bone marrow. Nat Protoc 4: 102-106.
31. Sun S, Guo Z, Xiao X, et al. 2003; Isolation of mouse marrow mesenchymal progenitors by a novel and reliable method. Stem Cells 21: 527-535.
32. Tropel P, Noel D, Platet N, et al. 2004; Isolation and characterisation of mesenchymal stem cells from adult mouse bone marrow. Exp Cell Res 295: 395-406.
33. Tsai CC, Chen CL, Liu HC, et al. 2010; Overexpression of hTERT increases stem-like properties and decreases spontaneous differentiation in human mesenchymal stem cell lines. J Biomed Sci 17: 64.
34. Tsai CC, Chen YJ, Yew TL, et al. 2011; Hypoxia inhibits senescence and maintains mesenchymal stem cell properties through down-regulation of E2A-p21 by HIF-TWIST. Blood 117: 459-469.
35. Van Vlasselaer P, Falla N, Snoeck H, et al. 1994; Characterization and purification of osteogenic cells from murine bone marrow by two-color cell sorting using anti-Sca-1 monoclonal antibody and wheat germ agglutinin. Blood 84: 753-763.
36. Wang CY, Yang HB, Hsu HS, et al. 2011; Mesenchymal stem cell-conditioned medium facilitates angiogenesis and fracture healing in diabetic rats. J Tissue Eng Regen Med doi: 10.1002/term.461.
37. Yew TL, Huang TF, Ma HL, et al. 2012; Scale-up of MSCs under hypoxic conditions for allogeneic transplantation and enhancing bony regeneration in a rabbit calvarial defect model. J Orthop Res doi: 10.1002/jor.22070.
38. Yew TL, Hung YT, Li HY, et al. 2011; Enhancement of wound healing by human multipotent stromal cell conditioned medium: the paracrine factors and p38MAPK activation. Cell Transpl 20: 693-706.
39. Zhu H, Guo ZK, Jiang XX, et al. 2010; A protocol for isolation and culture of mesenchymal stem cells from mouse compact bone. Nat Protoc 5: 550-560.