Scale-up of MSC under hypoxic conditions for allogeneic transplantation and enhancing bony regeneration in a rabbit calvarial defect model

Journal of Orthopaedic Research

Volumn 30, Issue 8, 2012, Pages 1213-1220

  Yew, Tu Lai ^a   Huang, Tung Fu ^a,b   Ma, Hsiao Li ^a,b   Hsu, Yuan Tong ^b   Tsai, Chih Chien ^a,b   Chiang, Chao Ching ^a,b   Chen, Wei Ming ^a,b   Hung, Shih Chieh ^a,b  

^a NATIONAL YANG MING UNIVERSITY   (Taiwan)

^b TAIPEI VETERANS GENERAL HOSPITAL   (Taiwan)

Author keywords

bone repair; calvarial defects; hypoxic culture; low density culture; mesenchymal stem cells; multipotent stromal cells; rabbit

Indexed keywords

BROXURIDINE;

ADIPOCYTE; ALLOTRANSPLANTATION; ANIMAL CELL; ANIMAL TISSUE; ARTICLE; BONE DEFECT; BONE REGENERATION; BONE REMODELING; BONE TISSUE; CALLUS; CALVARIA; CARTILAGE CELL; CELL DIFFERENTIATION; CELL DIVISION; CELL EXPANSION; CELL HYPOXIA; CELL ISOLATION; CELL MEMBRANE POTENTIAL; HISTOLOGY; MESENCHYMAL STEM CELL; MICRO-COMPUTED TOMOGRAPHY; NONHUMAN; OSTEOBLAST; PRIORITY JOURNAL; RABBIT; RADIOGRAPHY;

ANIMALS; BONE REGENERATION; CELL DIFFERENTIATION; CELL HYPOXIA; CELLS, CULTURED; MESENCHYMAL STEM CELLS; RABBITS; SKULL; TISSUE ENGINEERING; TRANSPLANTATION, HOMOLOGOUS; WOUND HEALING;

EID: 84862768249     PISSN: 07360266     EISSN: 1554527X     Source Type: Journal    
DOI: 10.1002/jor.22070     Document Type: Article

References (24)

1. Bruder SP, Fox BS., 1999. Tissue engineering of bone. Cell based strategies. Clin Orthop Relat Res 367: S68-S83.
2. Pittenger MF, Mackay AM, Beck SC, et al. 1999. Multilineage potential of adult human mesenchymal stem cells. Science 284: 143-147.
3. Prockop DJ., 1997. Marrow stromal cells as stem cells for nonhematopoietic tissues. Science 276: 71-74.
4. Bruder SP, Kraus KH, Goldberg VM, et al. 1998. The effect of implants loaded with autologous mesenchymal stem cells on the healing of canine segmental bone defects. J Bone Joint Surg Am 80: 985-996.
5. Bruder SP, Kurth AA, Shea M, et al. 1998. Bone regeneration by implantation of purified, culture-expanded human mesenchymal stem cells. J Orthop Res 16: 155-162.
6. Sakuragawa N., 1992. Alternating hemiplegia in childhood: 23 cases in Japan. Brain Dev 14: 283-288.
7. Kadiyala S, Young RG, Thiede MA, et al. 1997. Culture expanded canine mesenchymal stem cells possess osteochondrogenic potential in vivo and in vitro. Cell Transplant 6: 125-134.
8. Tsai CC, Chen YJ, Yew TL, et al. 2011. Hypoxia inhibits senescence and maintains mesenchymal stem cell properties through down-regulation of E2A-p21 by HIF-TWIST. Blood 117: 459-469.
9. Hung SC, Pochampally RR, Hsu SC, et al. 2007. Short-term exposure of multipotent stromal cells to low oxygen increases their expression of CX3CR1 and CXCR4 and their engraftment in vivo. PLoS ONE 2: e416.
10. Hung SC, Pochampally RR, Chen SC, et al. 2007. Angiogenic effects of human multipotent stromal cell conditioned medium activate the PI3K-Akt pathway in hypoxic endothelial cells to inhibit apoptosis, increase survival, and stimulate angiogenesis. Stem Cells 25: 2363-2370.
11. Yew TL, Hung YT, Li HY, et al. 2011. Enhancement of wound healing by human multipotent stromal cell conditioned medium: the paracrine factors and p38MAPK activation. Cell Transplant 20: 693-706.
12. Yew TL, Chiu FY, Tsai CC, et al. 2011. Knockdown of p21(Cip1/Waf1) enhances proliferation, the expression of stemness markers, and osteogenic potential in human mesenchymal stem cells. Aging Cell 10: 349-361.
13. Dominici M, Le Blanc K, Mueller I, et al. 2006. Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement. Cytotherapy 8: 315-317.
14. Guilak F, Cohen DM, Estes BT, et al. 2009. Control of stem cell fate by physical interactions with the extracellular matrix. Cell Stem Cell 5: 17-26.
15. Dashtdar H, Rothan HA, Tay T, et al. 2011. A preliminary study comparing the use of allogenic chondrogenic pre-differentiated and undifferentiated mesenchymal stem cells for the repair of full thickness articular cartilage defects in rabbits. J Orthop Res 29: 1336-1342.
16. Tolar J, Villeneuve P, Keating A., Mesenchymal stromal cells for graft-versus-host disease. Hum Gene Ther 22: 257-262.
17. Zhou Y, Wang S, Yu Z, et al. Marrow stromal cells differentiate into vasculature after allogeneic transplantation into ischemic myocardium. Ann Thorac Surg 91: 1206-1212.
18. Yagi H, Soto-Gutierrez A, Parekkadan B, et al. Mesenchymal stem cells: mechanisms of immunomodulation and homing. Cell Transplant 19: 667-679.
19. Engler AJ, Sen S, Sweeney HL, et al. 2006. Matrix elasticity directs stem cell lineage specification. Cell 126: 677-689.
20. Tsai KS, Kao SY, Wang CY, et al. 2010. Type I collagen promotes proliferation and osteogenesis of human mesenchymal stem cells via activation of ERK and Akt pathways. J Biomed Mater Res A 94: 673-682.
21. Lund AW, Stegemann JP, Plopper GE., 2009. Mesenchymal stem cells sense three dimensional type I collagen through discoidin domain receptor 1. Open Stem Cell J 1: 40-53.
22. Schantz JT, Hutmacher DW, Lam CX, et al. 2003. Repair of calvarial defects with customised tissue-engineered bone grafts II. Evaluation of cellular efficiency and efficacy in vivo. Tissue Eng 9. (Suppl 1) S127-S139.
23. Degano IR, Vilalta M, Bago JR, et al. 2008. Bioluminescence imaging of calvarial bone repair using bone marrow and adipose tissue-derived mesenchymal stem cells. Biomaterials 29: 427-437.
24. Wang CY, Yang HB, Hsu HS, et al. 2011. Mesenchymal stem cell-conditioned medium facilitates angiogenesis and fracture healing in diabetic rats. J Tissue Eng Regen Med Sep 13 (Published online only).