Development of a diphtheria toxin-based recombinant porcine IL-2 fusion toxin for depleting porcine CD25+ cells

Journal of Immunological Methods

Volumn 398-399, Issue 1, 2013, Pages 33-43

  Peraino, Jaclyn Stromp ^a   Schenk, Marian ^a   Li, Guoying ^a   Zhang, Huiping ^a   Farkash, Evan A ^a   Sachs, David H ^a   Huang, Christene A ^a   Duran Struuck, Raimon ^a   Wang, Zhirui ^a  

^a MASSACHUSETTS GENERAL HOSPITAL   (United States)

Author keywords

Diphtheria toxin; Fusion toxin; Pichia pastoris expression; Porcine IL 2; Regulatory T cell

Indexed keywords

DIPHTHERIA TOXIN; INTERLEUKIN 2 RECEPTOR GAMMA; RECOMBINANT INTERLEUKIN 2;

ANIMAL CELL; ANIMAL EXPERIMENT; ARTICLE; B CELL LYMPHOMA; BINDING AFFINITY; CD25+ T LYMPHOCYTE; CELL DEATH; CELL PROLIFERATION; CELL SURFACE; CONTROLLED STUDY; IMMUNOREGULATION; INTERNALIZATION; MEMBRANE BINDING; MOLECULAR INTERACTION; MOUSE; NONHUMAN; PERIPHERAL BLOOD MONONUCLEAR CELL; PORCINE MODEL; PRIORITY JOURNAL; PROTEIN SYNTHESIS; PROTEIN SYNTHESIS INHIBITION; SURVIVAL TIME; T CELL DEPLETION; TRANSPLANTATION TOLERANCE;

DIPHTHERIA TOXIN; FUSION TOXIN; PICHIA PASTORIS EXPRESSION; PORCINE IL-2; REGULATORY T CELL;

ANIMALS; DIPHTHERIA TOXIN; GLYCOSYLATION; HETEROGRAFTS; INTERLEUKIN-2; INTERLEUKIN-2 RECEPTOR ALPHA SUBUNIT; LYMPHOCYTE DEPLETION; LYMPHOMA, B-CELL; MICE; MICE, INBRED NOD; MICE, SCID; NEOPLASM TRANSPLANTATION; PROTEIN BIOSYNTHESIS; RECOMBINANT FUSION PROTEINS; SWINE; XENOGRAFT MODEL ANTITUMOR ASSAYS;

MUS; PICHIA PASTORIS; SUS;

EID: 84888200316     PISSN: 00221759     EISSN: 18727905     Source Type: Journal    
DOI: 10.1016/j.jim.2013.09.006     Document Type: Article

References (13)

1. Cho P.S., Lo D.P., Wikiel K.J., Rowland H.C., Coburn R.C., McMorrow I.M., Goodrich J.G., Arn J.S., Billiter R.A., Houser S.L., Shimizu A., Yang Y.G., Sachs D.H., Huang C.A. Establishment of transplantable porcine tumor cell lines derived from MHC-inbred miniature swine. Blood 2007, 110:3996.
2. Gritzapis A.D., Voutsas I.F., Baxevanis C.N. Ontak reduces the immunosuppressive tumor environment and enhances successful therapeutic vaccination in HER-2/neu-tolerant mice. Cancer Immunol Immunother 2012, 61:397.
3. Kim G.B., Wang Z., Liu Y.Y., Stavrou S., Mathias A., Goodwin K.J., Thomas J.M., Neville D.M. A fold-back single-chain diabody format enhances the bioactivity of an anti-monkey CD3 recombinant diphtheria toxin-based immunotoxin. Protein Eng. Des. Sel. 2007, 20:425.
4. Litzinger M.T., Fernando R., Curiel T.J., Grosenbach D.W., Schlom J., Palena C. IL-2 immunotoxin denileukin diftitox reduces regulatory T cells and enhances vaccine-mediated T-cell immunity. Blood 2007, 110:3192.
5. Mahnke K., Schönfeld K., Fondel S., Ring S., Karakhanova S., Wiedemeyer K., Bedke T., Johnson T.S., Storn V., Schallenberg S., Enk A.H. Depletion of CD4+CD25+ human regulatory T cells in vivo: kinetics of Treg depletion and alterations in immune functions in vivo and in vitro. Int. J. Cancer 2007, 120:2723.
6. Morse M.A., Hobeika A.C., Osada T., Serra D., Niedzwiecki D., Lyerly H.K., Clay T.M. Depletion of human regulatory T cells specifically enhances antigen-specific immune responses to cancer vaccines. Blood 2008, 112:610.
7. Peraino J., Zhang H., Hermanrud C.E., Li G., Sachs D.H., Huang C.A., Wang Z. Expression and purification of soluble porcine CTLA-4 in yeast Pichia pastoris. Protein Expr. Purif. 2012, 82:270.
8. Peraino J., Hermanrud C.E., Springett L., Zhang H., Li G., Srinivasan S., Gusha A., Sachs D.H., Huang C.A., Wang Z. Expression and characterization of recombinant soluble porcine CD3 ectodomain molecules: mapping the epitope of an anti-porcine CD3 monoclonal antibody 898H2-6-15. Cell. Immunol. 2012, 276:162.
9. Salagianni M., Lekka E., Moustaki A., Iliopoulou E.G., Baxevanis C.N., Papamichail M., Perez S.A. NK cell adoptive transfer combined with Ontak-mediated regulatory T cell elimination induces effective adaptive antitumor immune responses. J. Immunol. 2011, 186:3327.
10. Wang Z., Duran-Struuck R., Crepeau R., Matar A., Hanekamp I., Srinivasan S., Neville D.M., Sachs D.H., Huang C.A. Development of a diphtheria toxin based antiporcine CD3 recombinant immunotoxin. Bioconjug Chem. 2011, 22:2014.
11. Woo J.H., Liu Y.Y., Mathias A., Stavrou S., Wang Z., Thompson J., Neville D.M. Gene optimization is necessary to express a bivalent anti-human anti-T cell immunotoxin in Pichia pastoris. Protein Expr. Purif. 2002, 25:270.
12. Yamada Y., Aoyama A., Tocco G., Boskovic S., Nadazdin O., Alessandrini A., Madsen J.C., Cosimi A.B., Benichou G., Kawai T. Differential effects of denileukin diftitox IL-2 immunotoxin on NK and regulatory T cells in nonhuman primates. J. Immunol. 2012, 188:6063.
13. Zhang B., Ge B., Xia X., Liu J., Sun L., Rao E., Zhao Y. Compatibility of porcine and human interleukin 2: implications for xenotransplantation. Xenotransplantation 2006, 13:423.