Improving the chitinolytic activity of Bacillus pumilus SG2 by random mutagenesis

Journal of Microbiology and Biotechnology

Volumn 23, Issue 11, 2013, Pages 1519-1528

  Vahed, Majid ^a,b   Motalebi, Ebrahim ^a   Rigi, Garshasb ^a   Noghabi, Kambiz Akbari ^a   Soudi, Mohammad Reza ^c   Sadeghi, Mehdi ^a   Ahmadian, Gholamreza ^a  

^a NATIONAL INSTITUTE OF GENETIC ENGINEERING AND BIOTECHNOLOGY   (Iran)

^b ISLAMIC AZAD UNIVERSITY   (Iran)

^c ALZAHRA UNIVERSITY   (Iran)

Author keywords

AV2 9; Bacillus pumilus; Chitinase; Mutagenesis

Indexed keywords

CHITIN; CHITINASE; MUTANT PROTEIN; NITROUS ACID; RECOMBINANT CHITINASE L; RECOMBINANT CHITINASE S; RECOMBINANT ENZYME; SODIUM CHLORIDE; SPACER DNA; UNCLASSIFIED DRUG;

ANTIFUNGAL ACTIVITY; ARTICLE; BACILLUS PUMILUS; BACILLUS PUMILUS SG2; BACTERIAL GENE; BACTERIUM MUTANT; BIOINFORMATICS; BIOLOGICAL CONTROL AGENT; CHIL GENE; CHIS GENE; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; ENZYMATIC DEGRADATION; ENZYME ACTIVE SITE; ENZYME STABILITY; ENZYME STRUCTURE; ESCHERICHIA COLI; HYDROLYSIS; MUTAGENESIS; NONHUMAN; NUCLEOTIDE SEQUENCE; OPERON; PH; SATURATION MUTAGENESIS; SEQUENCE ANALYSIS; STRUCTURE ACTIVITY RELATION; TEMPERATURE; ULTRAVIOLET IRRADIATION; WILD TYPE;

AMINO ACID SEQUENCE; BACILLUS; CHITIN; CHITINASE; CLONING, MOLECULAR; DNA MUTATIONAL ANALYSIS; DNA, BACTERIAL; ENZYME STABILITY; ESCHERICHIA COLI; GENE EXPRESSION; HYDROLYSIS; METABOLIC ENGINEERING; MOLECULAR SEQUENCE DATA; MUTAGENESIS; NITROUS ACID; POINT MUTATION; RECOMBINANT PROTEINS; SEQUENCE ANALYSIS, DNA; ULTRAVIOLET RAYS;

EID: 84888194453     PISSN: 10177825     EISSN: 17388872     Source Type: Journal    
DOI: 10.4014/jmb.1301.01048     Document Type: Article

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