Quantitative detection and proliferation dynamics of a novel Spiroplasma eriocheiris pathogen in the freshwater crayfish, Procambarus clarkii

Journal of Invertebrate Pathology

Volumn 115, Issue 1, 2014, Pages 51-54

  Ding, Zhengfeng ^a,b   Tang, Jianqing ^b   Xue, Hui ^b   Li, Jiajia ^b   Ren, Qian ^a   Gu, Wei ^a   Meng, Qingguo ^a   Wang, Wen ^a  

^a NANJING NORMAL UNIVERSITY   (China)

^b FRESHWATER FISHERIES RESEARCH INSTITUTE OF JIANGSU PROVINCE   (China)

Author keywords

Quantitation; Real time PCR; Spiroplasma eriocheiris; SYBR Green

Indexed keywords

ASTACOIDEA; PROCAMBARUS CLARKII; SPIROPLASMA;

FRESH WATER;

ANIMAL; ARTICLE; CRAYFISH; CYTOLOGY; METHODOLOGY; MICROBIOLOGY; QUANTITATION; REAL TIME POLYMERASE CHAIN REACTION; REAL-TIME PCR; SPIROPLASMA; SPIROPLASMA ERIOCHEIRIS; SYBR GREEN;

QUANTITATION; REAL-TIME PCR; SPIROPLASMA ERIOCHEIRIS; SYBR GREEN;

ANIMALS; ASTACOIDEA; FRESH WATER; REAL-TIME POLYMERASE CHAIN REACTION; SPIROPLASMA;

EID: 84888101257     PISSN: 00222011     EISSN: 10960805     Source Type: Journal    
DOI: 10.1016/j.jip.2013.10.012     Document Type: Article

References (21)

1. Bi K., Huang H., Gu W., Wang J., Wang W. Phylogenetic analysis of spiroplasmas from three freshwater crustaceans (Eriocheir sinensis, Procambarus clarkii and Penaeus vannamei) in China. J. Invertebr. Pathol. 2008, 99:57-65.
2. Bowers R.M., Lapatra S.E., Dhar A.K. Detection and quantitation of infectious pancreatic necrosis virus by real-time reverse transcriptase-polymerase chain reaction using lethal and non-lethal tissue sampling. J. Virol. Methods 2008, 147:226-234.
3. Dhar A.K., Roux M.M., Klimpel K.R. Quantitative assay for measuring the Taura syndrome virus and yellow head virus load in shrimp by real-time RT-PCR using SYBR Green chemistry. J. Virol. Methods 2002, 104:69-82.
4. Ding Z., Bi K., Wu T., Gu W., Wang W., Chen J. A simple PCR method for the detection of pathogenic spiroplasmas in crustaceans and environmental samples. Aquaculture 2007, 265:49-54.
5. Ding Z., Du J., Ou J., Li W., Wu T., Xiu Y., Meng Q., Ren Q., Gu W., Xue H., Tang J., Wang W. Classification of circulating hemocytes from the red swamp crayfish Procambarus clarkii and their susceptibility to the novel pathogen Spiroplasma eriocheiris in vitro. Aquaculture 2012, 356-357:371-380.
6. Ding Z., Yao W., Du J., Ren Q., Li W., Wu T., Xiu Y., Meng Q., Gu W., Xue H., Tang J., Wang W. Histopathological characterization and in situ hybridization of a novel spiroplasma pathogen in the freshwater crayfish Procambarus clarkii. Aquaculture 2013, 380-383:106-113.
7. Du H.H., Hou C.L., Wu X.G., Xie R.h., Wang Y.-Z. Antigenic and immunogenic properties of truncated VP28 protein of white spot syndrome virus in Procambarus clarkii. Fish Shellfish Immunol. 2013, 34:332-338.
8. Goodwin A., Merry G., Sadler J. Detection of the herpesviral hematopoietic necrosis disease agent (Cyprinid herpesvirus 2) in moribund and healthy goldfish: validation of a quantitative PCR diagnostic method. Dis. Aquat. Organ 2006, 69:137-143.
9. Liang T., Li X., Du J., Yao W., Sun G., Dong X., Liu Z., Ou J., Meng Q., Gu W. Identification and isolation of a spiroplasma pathogens from diseased freshwater prawns, Macrobrachium rosenbergii, in China: a new freshwater crustacean host. Aquaculture 2011, 318:1-6.
10. Mackay I.M., Arden K.E., Nitsche A. Real-time PCR in virology. Nucl. Acids Res. 2002, 30:1292-1305.
11. Nunan L.M., Lightner D.V., Oduori M.A., Gasparich G.E. Spiroplasma penaei sp. nov., associated with mortalities in Penaeus vannamei, pacific white shrimp. Int. J. Syst. Evol. Micr. 2005, 55:2317-2322.
12. Rimmer A.E., Becker J.A., Tweedie A., Whittington R.J. Development of a quantitative polymerase chain reaction (qPCR) assay for the detection of dwarf gourami iridovirus (DGIV) and other megalocytiviruses and comparison with the Office International des Epizooties (OIE) reference PCR protocol. Aquaculture 2012, 358-359:155-163.
13. Sun Y., Yue Z., Liu H., Zhao Y., Liang C., Li Y., Shi X., Wu B., Xu B., Deng M. Development and evaluation of a sensitive and quantitative assay for hirame rhabdovirus based on quantitative RT-PCR. J. Virol. Methods 2010, 169:391-396.
14. Sun Y., Li F., Xiang J. Analysis on the dynamic changes of the amount of WSSV in Chinese shrimp Fenneropenaeus chinensis during infection. Aquaculture 2013, 376-379:124-132.
15. Tang K., Lightner D.V. Detection and quantification of infectious hypodermal and hematopoietic necrosis virus in penaeid shrimp by real-time PCR. Dis. Aquat. Organ 2001, 44:79-85.
16. Thompson J.D., Gibson T.J., Plewniak F., Jeanmougin F., Higgins D.G. The Clustal_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucl. Acids Res. 1997, 25:4876-4882.
17. Wang W., Wen B., Gasparich G.E., Zhu N., Rong L., Chen J., Xu Z. A spiroplasma associated with tremor disease in the Chinese mitten crab (Eriocheir sinensis). Microbiology 2004, 150:3035-3040.
18. Wang W., Gu W., Ding Z., Ren Y., Chen J., Hou Y. A novel spiroplasma pathogen causing systemic infection in the crayfish Procambarus clarkii (Crustacea: Decapod), in China. FEMS Microbiol. Lett. 2005, 249:131-137.
19. Wang J., Huang H., Feng Q., Liang T., Bi K., Gu W., Wang W., Shields J. Enzymelined immunosorbent assay for the detection of pathogenic spiroplasma in commercially exploited crustaceans from China. Aquaculture 2009, 292:166-171.
20. Wang W., Gu W., Gasparich G.E., Bi K., Ou J., Meng Q., Liang T., Feng Q., Zhang J., Zhang Y. Spiroplasma eriocheiris sp. nov., associated with mortality in the Chinese mitten crab, Eriocheir sinensis. Int. J. Syst. Evol. Micr. 2011, 61:703-708.
21. Whelan J.A., Russell N.B., Whelan M.A. A method for the absolute quantification of cDNA using real-time PCR. J. Immunol. Methods 2003, 278:261-269.