Efficient cellular fractionation improves RNA sequencing analysis of mature and nascent transcripts from human tissues

BMC Biotechnology

Volumn 13, Issue , 2013, Pages

  Zaghlool, Ammar ^a   Ameur, Adam ^a   Nyberg, Linnea ^a   Halvardson, Jonatan ^a   Grabherr, Manfred ^a   Cavelier, Lucia ^a   Feuk, Lars ^a  

^a UPPSALA UNIVERSITY   (Sweden)

Author keywords

Cytoplasmic RNA; De novo assembly; Nascent transcripts; Nuclear RNA; PolyA+ selection; RNA purification; RNA sequencing; RNA splicing; Transcription profiling; Transcriptomics

Indexed keywords

CYTOPLASMIC RNA; DE NOVO ASSEMBLIES; NASCENT TRANSCRIPTS; NUCLEAR RNA; POLYA+ SELECTION; RNA SEQUENCING; RNA SPLICING; TRANSCRIPTION PROFILING; TRANSCRIPTOMICS;

GENE EXPRESSION; HISTOLOGY; MAMMALS; PURIFICATION; SENSITIVITY ANALYSIS; TISSUE;

RNA;

CYTOPLASMIC RNA; NUCLEAR RNA; POLYADENYLIC ACID; RNA; TRANSCRIPTOME; UNCLASSIFIED DRUG;

ARTICLE; CELL FRACTIONATION; CYTOSOL; EXON; FEMALE; FETUS; GENE EXPRESSION; GENETIC TRANSCRIPTION; HUMAN; HUMAN TISSUE; INTRON; MALE; RNA ANALYSIS; RNA PROCESSING; RNA PURIFICATION; RNA SEQUENCE; RNA SPLICING;

MAMMALIA;

CELL LINE; CYTOPLASM; EXONS; GENE EXPRESSION; GENE EXPRESSION PROFILING; HUMANS; INTRONS; RNA SPLICING; RNA, MESSENGER; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS, RNA; TRANSCRIPTOME;

EID: 84887992974     PISSN: None     EISSN: 14726750     Source Type: Journal    
DOI: 10.1186/1472-6750-13-99     Document Type: Article

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