Electrophysiological integration and action potential properties of transplanted cardiomyocytes derived from induced pluripotent stem cells

Cardiovascular Research

Volumn 100, Issue 3, 2013, Pages 432-440

  Halbach, Marcel ^a   Peinkofer, Gabriel ^a   Baumgartner, Sven ^a   Maass, Martina ^a   Wiedey, Mirjam ^a   Neef, Klaus ^a   Krausgrill, Benjamin ^a   Ladage, Dennis ^a   Fatima, Azra ^a   Saric, Tomo ^a   Hescheler, Jürgen ^a   Müller Ehmsen, Jochen ^a  

^a UNIVERSITY HOSPITAL OF COLOGNE   (Germany)

Author keywords

Conduction; Electrophysiology; I PSCM; Transplantation

Indexed keywords

ANTIBIOTIC AGENT; ENHANCED GREEN FLUORESCENT PROTEIN; MYOSIN HEAVY CHAIN ALPHA; PUROMYCIN;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; CARDIAC CELL REPLACEMENT THERAPY; CELL DIFFERENTIATION; CELL MATURATION; CELL MEMBRANE DEPOLARIZATION; CELL STRUCTURE; CELL TRANSPLANTATION; CONTROLLED STUDY; HEART CONTRACTION; HEART ELECTROPHYSIOLOGY; HEART MUSCLE CELL; HEART MUSCLE FIBER MEMBRANE POTENTIAL; HISTOLOGY; HOST; IN VITRO STUDY; MALE; MICROELECTRODE; MOUSE; NONHUMAN; PLURIPOTENT STEM CELL; PRIORITY JOURNAL; PROTEIN EXPRESSION;

CONDUCTION; ELECTROPHYSIOLOGY; IPSCM; TRANSPLANTATION;

ACTION POTENTIALS; ANIMALS; CELL DIFFERENTIATION; CELL LINE; CELL SURVIVAL; GREEN FLUORESCENT PROTEINS; INDUCED PLURIPOTENT STEM CELLS; MALE; MICE; MICE, 129 STRAIN; MICE, INBRED C57BL; MYOCYTES, CARDIAC; MYOSIN HEAVY CHAINS; PROMOTER REGIONS, GENETIC; TIME FACTORS; TRANSFECTION; VENTRICULAR MYOSINS;

EID: 84887913513     PISSN: 00086363     EISSN: 17553245     Source Type: Journal    
DOI: 10.1093/cvr/cvt213     Document Type: Article

References (28)

1. Kolossov E, Bostani T, Roell W., Breitbach M, Pillekamp F, Nygren JM et al. Engraftment of engineered ES cell-derived cardiomyocytes but not BM cells restores contractile function to the infarcted myocardium. J Exp Med 2006; 203:2315-2327.
2. Zhang J, Wilson GF, Soerens A.G., Koonce CH, Yu J, Palecek SP et al. Functional cardio-myocytes derived from human induced pluripotent stem cells. Circ Res 2009; 104: e30-e41.
3. Kolossov E, Lu Z, Drobinskaya I., Gassanov N, Duan Y, Sauer H et al. Identification and characterization of embryonic stem cell-derived pacemaker and atrial cardiomyocytes. FASEB J 2005; 19:577-579.
4. Wang L, Feng ZP, Kondo C.S., Sheldon RS, Duff HJ Developmental changesinthe delayed rectifier K+ channels in mouse heart. Circ Res 1996; 79:79-85.
5. Wang L, Duff HJ Developmental changes in transient outward current in mouse ventricle. Circ Res 1997; 81:120-127.
6. Singla DK, Long X, Glass C., Singla RD, Yan B. Induced pluripotent stem (i PS) cells repair and regenerate infarcted myocardium. Mol Pharm 2011; 8:1573-1581.
7. Yan B, Abdelli LS, Singla DK Transplanted induced pluripotent stem cells improve cardiac function and induce neovascularization in the infarcted hearts of db/db mice. Mol Pharm 2011; 8:1602-1610.
8. Nelson TJ, Martinez-Fernandez A, Yamada S., Perez-Terzic C, Ikeda Y, Terzic A. Repair of acute myocardial infarction by human stemness factors induced pluripotent stem cells. Circulation 2009; 120:408-416.
9. Templin C, Zweigerdt R, Schwanke K., Olmer R, Ghadri JR, Emmert MY et al. Transplantation and tracking of human-induced pluripotent stem cells in a pig model of myocardial infarction: assessment of cell survival, engraftment, and distribution by hybrid single photonemission computed tomography/computed tomography of sodium iodide sym-porter transgene expression. Circulation 2012; 126:430-439.
10. Smits AM, van Laake LW, den Ouden K, Schreurs C, Szuhai K., van Echteld CJ et al. Human cardiomyocyte progenitor cell transplantation preserves long-term function of the infarcted mouse myocardium. Cardiovasc Res 2009; 83:527-535.
11. Mauritz C, Martens A, Rojas S.V., Schnick T., Rathert C, Schecker N et al. Induced pluripo-tent stem cell (i PSC)-derived Flk-1 progenitor cells engraft, differentiate, and improve heart function in a mouse model of acute myocardial infarction. Eur Heart J 2011; 32: 2634-2641.
12. Zwi-Dantsis L., Huber I, Habib M., Winterstern A, Gepstein A, Arbel G., Gepstein L. Derivation and cardiomyocyte differentiation of induced pluripotent stem cells from heart failure patients. Eur Heart J 2013; 34:1575-1586 doi:10.1093/eurheartj/ehs096.
13. Halbach M, Pfannkuche K, Pillekamp F., Ziomka A, Hannes T, Reppel M et al. Electro-physiological maturation and integration of murine fetal cardiomyocytes after transplantation. Circ Res 2007; 101:484-492.
14. Halbach M, Krausgrill B, Hannes T., Wiedey M, Peinkofer G, Baumgartner S et al. Time-course of the electrophysiological maturation and integration of transplanted cardio-myocytes. J Mol Cell Cardiol 2012; 53:401-408.
15. Meissner A, Wernig M, Jaenisch R. Direct reprogramming of genetically unmodified fibroblasts into pluripotent stem cells. Nat Biotechnol 2007; 25:1177-1181.
16. Kuzmenkin A, Liang H, Xu G., Pfannkuche K, Eichhorn H, Fatima A et al. Functional characterization of cardiomyocytes derived from murine induced pluripotent stem cells in vitro. FASEB J 2009; 23:4168-4180.
17. Pfannkuche K, Liang H, Hannes T., Xi J, Fatima A, Nguemo F et al. Cardiac myocytes derived from murine reprogrammed fibroblasts: intact hormonal regulation, cardiac ion channel expression and development of contractility. Cell Physiol Biochem 2009; 24: 73-86.
18. Roell W, Lu ZJ, Bloch W, Siedner S., Tiemann K, Xia Y, Fleischmann BK et al. Cellular car-diomyoplasty improves survival after myocardial injury. Circulation 2002; 105: 2435-2441.
19. Pillekamp F, Reppel M, Dinkelacker V., Duan Y, Jazmati N, Bloch W et al. Establishment and characterization of a mouse embryonic heart slice preparation. Cell Physiol Biochem 2005; 16:127-132.
20. Halbach M, Pillekamp F, Brockmeier K., Hescheler J, Muller-Ehmsen J, Reppel M. Ventricular slices of adult mouse hearts-a new multicellular invitro model for electrophysio-logical studies. Cell Physiol Biochem 2006; 18:1-8.
21. Gepstein L, Ding CH, Rahmutula D, Wilson E.E., Yankelson L., Caspi O et al. In vivo assessment of the electrophysiological integration and arrhythmogenic risk of myocardial cell transplantation strategies (Vol. 28, p. 2151, 2010). Stem Cells 2011; 29:1475.
22. Shiba Y, Fernandes S, Zhu W.Z., Filice D., Muskheli V, Kim J et al. Human ES-cell-derived cardiomyocytes electrically couple and suppress arrhythmias in injured hearts. Nature 2012; 489:322-325.
23. Rubart M, Pasumarthi KB, Nakajima H, Soonpaa M.H., Nakajima HO, Field LJ Physiological coupling of donor and host cardiomyocytes aftercellular transplantation. Circ Res 2003; 92:1217-1224.
24. Roell W, Lewalter T, Sasse P., Tallini YN, Choi BR, Breitbach M et al. Engraftment of connexin 43-expressing cells prevents post-infarct arrhythmia. Nature 2007; 450: 819-824.
25. Reinecke H, Zhang M, Bartosek T., Murry CE Survival, integration, and differentiation of cardiomyocyte grafts a study in normal and injured rat hearts. Circulation 1999; 100: 193-202.
26. Antzelevitch C, Yan GX, Shimizu W. Transmural dispersion of repolarization and arrhythmogenicity: the Brugada syndrome vs. the long QT syndrome. J Electrocardiol 1999; 32(suppl):158-165.
27. Liao SY, Liu Y, Siu C.W., Zhang Y., Lai WH, Au KW et al. Proarrhythmic risk of embryonic stem cell-derived cardiomyocyte transplantation in infarcted myocardium. Heart Rhythm 2010; 7:1852-1859.
28. Pillekamp F, Halbach M, Reppel M., Pfannkuche K, Nazzal R, Nguemo F et al. Physiological differences between transplanted and host tissue cause functional decoupling after in vitro transplantation of human embryonic stem cell-derived cardiomyocytes. Cell Physiol Biochem 2009; 23:65-74.