Efficient generation of recombinant RNA viruses using targeted recombination-mediated mutagenesis of bacterial artificial chromosomes containing full-length cDNA

BMC Genomics

Volumn 14, Issue 1, 2013, Pages

  Rasmussen, Thomas B ^a   Risager, Peter C ^a   Fahnøe, Ulrik ^a   Friis, Martin B ^a   Belsham, Graham J ^a   Höper, Dirk ^b   Reimann, Ilona ^b   Beer, Martin ^b  

^a TECHNICAL UNIVERSITY OF DENMARK   (Denmark)

^b FEDERAL RESEARCH INSTITUTE FOR ANIMAL HEALTH   (Germany)

Author keywords

Bacterial artificial chromosome; Classical swine fever virus; Genetic stability; Genome; Pestivirus; RNA; RNA virus; Targeted recombination

Indexed keywords

ASPARTIC ACID; CLASSICAL SWINE FEVER VIRUS VACCINE; COMPLEMENTARY DNA; GLYCINE; LIVE VACCINE; RNA DIRECTED RNA POLYMERASE; UNCLASSIFIED DRUG; VIRUS DNA; VIRUS PROTEIN; VIRUS VACCINE;

AMINO ACID SUBSTITUTION; ANIMAL CELL; ARTICLE; BACTERIAL ARTIFICIAL CHROMOSOME; CHIMERA; DNA MODIFICATION; GENE SEQUENCE; GENETIC MANIPULATION; GENETIC RECOMBINATION; GENETIC STABILITY; MAMMAL CELL; MOLECULAR CLONING; MUTAGENESIS; NONHUMAN; NUCLEOTIDE SEQUENCE; PESTIVIRUS; SEQUENCE ANALYSIS; TARGETED RECOMBINATION MEDIATED MUTAGENESIS; VIRUS GENOME; VIRUS STRAIN;

BACTERIA (MICROORGANISMS); CLASSICAL SWINE FEVER VIRUS; MAMMALIA; PESTIVIRUS; RNA VIRUSES; TOMATO ASPERMY VIRUS;

ANIMALS; BASE SEQUENCE; CHROMOSOMES, ARTIFICIAL, BACTERIAL; CLASSICAL SWINE FEVER VIRUS; CLONING, MOLECULAR; DNA, COMPLEMENTARY; MUTAGENESIS; OPEN READING FRAMES; RECOMBINATION, GENETIC; RNA; RNA VIRUSES; SWINE;

EID: 84887864273     PISSN: None     EISSN: 14712164     Source Type: Journal    
DOI: 10.1186/1471-2164-14-819     Document Type: Article

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