An Alternative Direct Compound Dispensing Method Using the HP D300 Digital Dispenser

Journal of Laboratory Automation

Volumn 18, Issue 5, 2013, Pages 367-374

  Jones, Raisa E ^a   Zheng, Wei ^a   McKew, John C ^a   Chen, Catherine Z ^a  

^a NATIONAL INSTITUTES OF HEALTH   (United States)

Author keywords

compound dispenser; compound screen; direct dilution; liquid handler; serial dilution

Indexed keywords

BIOCHEMICAL ASSAY; CYTOTOXICITY ASSAYS; DRUG DEVELOPMENT; DRUG DISCOVERY; INK JET TECHNOLOGY; LIQUID HANDLERS; REPORTER GENE ASSAYS; SERIAL DILUTIONS;

DRUG PRODUCTS; INSTRUMENTS;

DISPENSERS;

DAUNORUBICIN; DEXAMETHASONE; DIMETHYL SULFOXIDE; LANSOPRAZOLE; PREDNISOLONE; TAMOXIFEN;

AQUEOUS SOLUTION; ARTICLE; CONCENTRATION RESPONSE; CONTROLLED STUDY; CYTOTOXICITY; DIGITAL DISPENSER; DILUTION; DRUG RESEARCH; DRUG SCREENING; ENZYME ACTIVITY; ENZYME ASSAY; IN VITRO STUDY; LABORATORY DEVICE; MICROFLUIDICS; NONHUMAN; PIEZOELECTRICITY; PROCESS OPTIMIZATION; REPORTER GENE;

COMPOUND DISPENSER; COMPOUND SCREEN; DIRECT DILUTION; LIQUID HANDLER; SERIAL DILUTION;

AUTOMATION, LABORATORY; DRUG EVALUATION, PRECLINICAL; HIGH-THROUGHPUT SCREENING ASSAYS; REPRODUCIBILITY OF RESULTS; SPECIMEN HANDLING;

EID: 84887425783     PISSN: 22110682     EISSN: 22110690     Source Type: Journal    
DOI: 10.1177/2211068213491094     Document Type: Article

References (9)

1. Zheng W.,Chen C. Z.A Practical Guide to Assay Development and High-Throughput Screening in Drug Discovery. Chen T., ed. Boca Raton, FL: CRC Press; 2009:183-192.
2. Shi Z. D.,Motabar O.,Goldin E.,et al.Synthesis and Characterization of a New Fluorogenic Substrate for Alpha-Galactosidase.Anal. Bioanal. Chem. 2009;394:1903-1909.
3. Fan J. Q.,Ishii S.,Asano N.,Suzuki Y.Accelerated Transport and Maturation of Lysosomal Alpha-Galactosidase A in Fabry Lymphoblasts by an Enzyme Inhibitor.Nat. Med. 1999;5:112-115.
4. Motabar O.,Liu K.,Southall N.,et al.High Throughput Screening for Inhibitors of Alpha-Galactosidase.Curr. Chem. Genom. 2010;4:67-73.
5. Nehme A.,Lobenhofer E. K.,Stamer W. D.,Edelman J. L.Glucocorticoids with Different Chemical Structures but Similar Glucocorticoid Receptor Potency Regulate Subsets of Common and Unique Genes in Human Trabecular Meshwork Cells.BMC Med. Genom. 2009;2:58.
6. Cho M. H.,Niles A.,Huang R.,et al.A Bioluminescent Cytotoxicity Assay for Assessment of Membrane Integrity Using a Proteolytic Biomarker.Toxicol. In Vitro. 2008;22:1099-1106.
7. Xia M.,Huang R.,Witt K. L.,et al.Compound Cytotoxicity Profiling Using Quantitative High-Throughput Screening.Environ. Health Perspect. 2008;116:284-291.
8. Grant R. J.,Roberts K.,Pointon C.,et al.Achieving Accurate Compound Concentration in Cell-Based Screening: Validation of Acoustic Droplet Ejection Technology.J. Biomol. Screen. 2009;14:452-259.
9. Harris D.,Olechno J.,Datwani S.,Ellson R.Gradient, Contact-Free Volume Transfers Minimize Compound Loss in Dose-Response Experiments.J. Biomol. Screen. 2010;15:86-94.