Achieving accurate compound concentration in cell-based screening: Validation of acoustic droplet ejection technology

Journal of Biomolecular Screening

Volumn 14, Issue 5, 2009, Pages 452-459

  Grant, Richard John ^a   Roberts, Karen ^a   Pointon, Carly ^a   Hodgson, Clare ^a   Womersley, Lynsey ^a   Jones, Darren Craig ^a   Tang, Eric ^a  

^a ASTRAZENECA   (United Kingdom)

Author keywords

Acoustic droplet ejection; Cell based; Compound handling; Dose response; Echo; Nanoliter dispensing; Noncontact; Serial dilution

Indexed keywords

CHEMICAL COMPOUND; DIMETHYL SULFOXIDE;

ACCURACY; ARTICLE; BIOASSAY; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; DRUG SCREENING; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; HUMAN CELL; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; MATERIALS HANDLING; PRIORITY JOURNAL; VALIDATION PROCESS;

BIOLOGICAL ASSAY; CELL LINE; DIMETHYL SULFOXIDE; DRUG DISCOVERY; HUMANS; PROTO-ONCOGENE PROTEINS C-AKT; REPRODUCIBILITY OF RESULTS; SOLVENTS; SOUND; TECHNOLOGY, PHARMACEUTICAL;

EID: 67449108090     PISSN: 10870571     EISSN: 1552454X     Source Type: Journal    
DOI: 10.1177/1087057109336588     Document Type: Article

References (7)

1. Spicer T., Fitzgerald Y., Burford N., Matson S., Chatterjee M., Gilchrist M., et al. Pharmacological evaluation of different compound dilution and transfer paradigms on an enzyme assay in low volume 384-well format. Poster presented at Drug Discovery Technology, Boston, August 2005.
2. Ellson R., Mutz M., Browning B., Lee L., Miller M., Papan R.: Transfer of low nanoliter volumes between microplates using focused acoustics: automation considerations. J Assoc Lab Automation 2003 ; 8 (5). 29-34. (Pubitemid 38181559)
3. Olechno J., Shieh J., Ellson R.: Improving IC50 results with acoustic droplet ejection. J Assoc Lab Automation 2006 ; 11: 240-246. (Pubitemid 44184992)
4. Harris D., Mutz M.: Debunking the myth: validation of fluorescein for testing the precision of nanoliter dispensing. J Assoc Lab Automation 2006 ; 11: 233-239. (Pubitemid 44184977)
5. Gilchrist M., Cacace A., Harden D.: Characterization of the 5-HT2b receptor in evaluation of aequorin detection of calcium mobilization for miniaturized GPCR high-throughput screening. J Biomol Screen 2008 ; 13: 486-493.
6. Quintero C., Rosenstein C., Hughes B., Middleton R., Kariv, I.: Quality control procedures for dose-response curve generation using nanoliter dispense technologies. J Biomol Screen 2007 ; 12: 891-899. (Pubitemid 47340696)
7. Popa-Burke IG, Issakova O., Arroway JD, Bernasconi P., Chen M., Coudurier L., et al. Streamlined system for purifying and quantifying a diverse library of compounds and the effect of compound concentration measurements on the accurate interpretation of biological assay results. Anal Chem 2004 ; 76: 7278-7287. (Pubitemid 39657998)