Development of a specific siRNA delivery system into HeLa cells using an IgG-binding fusion protein

Biotechnology Letters

Volumn 35, Issue 12, 2013, Pages 2081-2089

  Bae, JooYoun ^a   Mie, Masayasu ^a   Kobatake, Eiry ^a  

^a TOKYO INSTITUTE OF TECHNOLOGY   (Japan)

Author keywords

Double stranded RNA binding domain; HeLa cells; Polyethyleneimine; Protein G; siRNA delivery

Indexed keywords

DOUBLE-STRANDED RNA; HELA CELL; POLYETHYLENEIMINE; PROTEIN G; SIRNA DELIVERY;

ANTIBODIES; GENE TRANSFER;

RNA;

BACTERIAL PROTEIN; GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; IGG FC BINDING PROTEIN, STREPTOCOCCUS; IGG FC-BINDING PROTEIN, STREPTOCOCCUS; IMMUNOGLOBULIN G; POLYETHYLENEIMINE; SMALL INTERFERING RNA;

ARTICLE; BINDING SITE; CELL SURVIVAL; GENE SILENCING; GENETIC TRANSFECTION; GENETICS; HELA CELL LINE; HUMAN; METABOLISM; METHODOLOGY;

BACTERIAL PROTEINS; BINDING SITES; CELL SURVIVAL; GENE SILENCING; GREEN FLUORESCENT PROTEINS; HELA CELLS; HUMANS; IMMUNOGLOBULIN G; POLYETHYLENEIMINE; RECOMBINANT FUSION PROTEINS; RNA, SMALL INTERFERING; TRANSFECTION;

EID: 84887317195     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-013-1299-y     Document Type: Article

References (12)

1. Bevilacqua PC, Cech TR (1996) Minor-groove recognition of double-stranded RNA by the double-stranded RNA-binding domain from the RNA-activated protein kinase PKR. Biochemistry 35: 9983-9994.
2. Boussif O, Lezoualc'h F, Zanta MA, Mergny MD, Scherman D, Demeneix B, Behr JP (1995) A versatile vector for gene and oligonucleotide transfer into cells in culture and in vivo: polyethtlenimine. Proc Natl Acad Sci USA 92: 7297-7301.
3. Han JY, Choi DS, Kim CH, Joo H, Min CK (2008) Selective gene transfer to endometrial cancer cells by a polymer against matrix metalloproteinase 2 (MMP-2). Cancer Biother Radiopharm 22: 247-258.
4. Hattori Y, Maitani Y (2007) Low-molecular-weight polyethyleneimine enhanced gene transfer by cationic cholesterol-based nanoparticle vector. Biol Pharm Bull 30: 1773-1778.
5. Jiang G, Park K, Kim J, Kim KS, Hahn SK (2009) Target specific intracellular delivery of siRNA/PEI-HA complex by receptor mediated endocytosis. Mol Pharm 6: 727-737.
6. Kong WH, Bae KH, Hong CA, Lee Y, Hahn SK, Park TG (2011) Multimerized siRNA cross-linked by gold nanoparticles. Bioconjugate Chem 22: 1962-1969.
7. Lee SY, Huh MS, Lee S, Lee SJ, Chung H, Park JH, Oh YK, Choi K, Kinm K, Kwon IC (2010) Stability and cellular uptake of polymerized siRNA (poly-siRNA)/polyethyleneimine (PEI) complexes for efficient gene silencing. J Control Release 141: 339-346.
8. Mao S, Neu M, Germershaus O, Merkel O, Sitterberg J, Barkowsky U, Kissel T (2006) Influence of polyethylene glycol chain length on the physicochemical and biological properties of poly(ethylene imine)-graft-poly(ethylene glycol) block copolymer/siRNA polyplexes. Bioconjugate Chem 17: 1209-1218.
9. Strehblow C, Schuster M, Moritz T, Kirch HC, Opalka B, Petri JB (2005) Monoclonal antibody-polyethyleneimine conjugates targeting Her-2/neu or CD90 allow cell type-specific nonviral gene delivery. J Control Release 102: 737-747.
10. Tan SJ, Kiatwuthinon P, Roh YH, Kahn JS, Luo D (2011) Engineering nanocarriers for siRNA delivery. Small 7: 841-856.
11. Tanaka G, Funabashi H, Mie M, Kobatake E (2006) Fabrication of an antibody microwell array with self-adhering antibody binding protein G. Anal Biochem 350: 298-303.
12. Wang Y, Li Z, Han Y, Liang LH, Ji A (2010) Nanoparticle-based delivery system for application of siRNA in vivo. Curr Drug Metab 11: 182-196.