Isolation of RNA from equine peripheral blood cells: Comparison of methods

SpringerPlus

Volumn 2, Issue 1, 2013, Pages 1-6

  Jiang, Zibin ^a   Uboh, Cornelius E ^a,b   Chen, Jinwen ^a   Soma, Lawrence R ^a  

^a UNIVERSITY OF PENNSYLVANIA   (United States)

^b WEST CHESTER UNIVERSITY   (United States)

Author keywords

Blood; Equine; LeukoLOCK ; Method comparison; RNA extraction

Indexed keywords

EID: 84887308221     PISSN: None     EISSN: 21931801     Source Type: Journal    
DOI: 10.1186/2193-1801-2-478     Document Type: Article

References (12)

1. Catts VS, Catts SV, Fernandez HR, Taylor JM, Coulson EJ, Lutze-Mann LH (2005) A microarray study of post-mortem mRNA degradation in mouse brain tissue. Brain Res Mol Brain Res 138:164-177.
2. Deng MY, Wang H, Ward GB, Bechham TR, McKenna TS (2005) Comparison of six RNA extraction methods for the detection of classical swine fever virus by real-time and conventional reverse transcription-PCR. J Vet Diagn Invest 17:574-578.
3. Dumur CI, Best NS, Archer AM, Ladd KJ, Mas AC, Wilkinson VR (2004) Evaluation of quality-control criteria for microarray gene expression analysis. Clin Chem 50:1994-2002.
4. Fleige S, Pfaffl MW (2006) RNA integrity and the effect on the realtime qRT-PCR performance. Mol Aspect of Med 27:126-139.
5. Glasel JA (1995) Validity of nucleic acid purities monitored by 260 nm/280 nm absorbance ratios. Biotechniques 8:62-63.
6. Guesella J, Varsanyi-Breiner A, Kao FT, Jones C, Puck T, Orkin S, Housman D (1979) Precise localization of human beta globin gene complex on chromosome I I. Proc NatI Acad Sci USA 76:5239.
7. Manchester KL (1996) Use of UV methods for measurement of protein and nucleic acid concentrations. Biotechniques 20:968-970.
8. Hammerle-Fickinger A, Riedmaier I, Becker C, Meyer HHD, Pfaffl MW, Ulbrich SE (2010) Validation of extraction methods for toal RNA and miRNA from bovine blood prior to quantitative gene expression analyses. Biotechnol Lett 32:35-44.
9. Schroeder A, Mueller O, Stocker S, Salowsky R, Leiber M, Gassmann M, Lightfoot S, Menzel W, Granzow M, Ragg T (2006) The RIN: an RNA integrity number for assigning integrity values to RNA measurements. BMC Mol Biol 31(7):3.
10. Strand C, Enell J, Hedenfalk I, Fernö M (2007) RNA quality in frozen breast cancer samples and the influence on gene expression analysis-a comparison of three evaluation methods using microcapillary electrophoresis traces. BMC Mol Biol 8:38.
11. Winn ME, Zapala MA, Hovatta I, Risbrough VB, Lillie E, Schork NJ (2010) The effects of globin on microarray-based gene expression analysis of mouse blood. Mamm Genome 21(5-6):268-275.
12. Wright C, Bergstrom D, Dai H, Marton M, Morris M, Tokiwa G, Wang Y, Fare T (2008) Characterization of globin RNA interference in gene expression profiling of whole-blood samples. Clin Chem 54(2):396-405.