Recombineering: Using drug cassettes to knock out genes in vivo

Methods in Enzymology

Volumn 533, Issue , 2013, Pages 79-102

  Sawitzke, James A ^a   Thomason, Lynn C ^a   Bubunenko, Mikhail ^a   Li, Xintian ^a   Costantino, Nina ^a   Court, Donald L ^a  

^a NATIONAL CANCER INSTITUTE   (United States)

Author keywords

Bacterial artificial chromosomes (BACs); Confirming knockout mutations; Design construct and order primers; Genes knock out; Linear substrate by polymerase chain reaction; Recombineering ready cells; Selecting for knockout mutations

Indexed keywords

ARTICLE; BACTERIAL ARTIFICIAL CHROMOSOME; BACTERIAL CHROMOSOME; CULTURE TECHNIQUE; ELECTROTRANSFORMATION; FUNCTIONAL GENOMICS; GENE; GENE FUNCTION; GENE MUTATION; GENETIC ENGINEERING; HOMOLOGOUS RECOMBINATION; HUMAN; IN VIVO STUDY; KNOCKOUT GENE; MUTAGENESIS; NONHUMAN; OPEN READING FRAME; PLASMID; PRIORITY JOURNAL; RECOMBINEERING; RESTRICTION SITE;

BACTERIA (MICROORGANISMS);

BACTERIAL ARTIFICIAL CHROMOSOMES (BACS); CONFIRMING KNOCKOUT MUTATIONS; DESIGN CONSTRUCT AND ORDER PRIMERS; GENES KNOCK OUT; LINEAR SUBSTRATE BY POLYMERASE CHAIN REACTION; RECOMBINEERING- READY CELLS; SELECTING FOR KNOCKOUT MUTATIONS;

ANTI-BACTERIAL AGENTS; BACTERIOPHAGE LAMBDA; CHROMOSOMES, BACTERIAL; DNA PRIMERS; DNA-BINDING PROTEINS; DRUG RESISTANCE, BACTERIAL; GENE KNOCKOUT TECHNIQUES; GENETIC ENGINEERING; MUTATION; PLASMIDS; POLYMERASE CHAIN REACTION; VIRAL PROTEINS;

EID: 84887255340     PISSN: 00766879     EISSN: 15577988     Source Type: Book Series    
DOI: 10.1016/B978-0-12-420067-8.00007-6     Document Type: Chapter

References (20)

1. A. Ahmed, and L. Podemski The revised nucleotide sequence of Tn5 Gene 154 1995 129 130
2. M. Bubunenko, T. Baker, and D.L. Court Essentiality of ribosomal and transcription antitermination proteins analyzed by systematic gene replacement in Escherichia coli Journal of Bacteriology 189 2007 2844 2853
3. S. Datta, N. Costantino, and D.L. Court A set of recombineering plasmids for gram-negative bacteria Gene 379 2006 109 115
4. S. Datta, N. Costantino, X. Zhou, and D.L. Court Identification and analysis of recombineering functions from Gram-negative and Gram-positive bacteria and their phages Proceedings of the National Academy of Sciences of the United States of America 105 2008 1626 1631
5. W. Hillen, and K. Schollmeier Nucleotide sequence of the Tn10 encoded tetracycline resistance gene Nucleic Acids Research 11 1983 525 539
6. M.E. Kovach, R.W. Phillips, P.H. Elzer, R.M. Roop 2nd, and K.M. Peterson pBBR1MCS: A broad-host-range cloning vector BioTechniques 16 1994 800 802
7. E.C. Lee, D. Yu, and J. Martinez de Velasco A highly efficient Escherichia coli-based chromosome engineering system adapted for recombinogenic targeting and subcloning of BAC DNA Genomics 73 2001 56 65
8. S.K. Sharan, L.C. Thomason, S.G. Kuznetsov, and D.L. Court Recombineering: A homologous recombination-based method of genetic engineering Nature Protocols 4 2009 206 223
9. S.L. Svenningsen, N. Costantino, D.L. Court, and S. Adhya On the role of Cro in lambda prophage induction Proceedings of the National Academy of Sciences of the United States of America 102 2005 4465 4469
10. L.C. Thomason, N. Costantino, D.V. Shaw, and D.L. Court Multicopy plasmid modification with phage lambda Red recombineering Plasmid 58 2007 148 158
11. S. Warming, N. Costantino, D.L. Court, N.A. Jenkins, and N.G. Copeland Simple and highly efficient BAC recombineering using galK selection Nucleic Acids Research 33 2005 e36
12. D. Yu, H.M. Ellis, E.C. Lee, N.A. Jenkins, N.G. Copeland, and D.L. Court An efficient recombination system for chromosome engineering in Escherichia coli Proceedings of the National Academy of Sciences of the United States of America 97 2000 5978 5983
13. Sawitzke JA, Thomason LC, Costantino N, Bubunenko M, Li X, and Court DL (chapter submitted) Recombineering: A modern approach to genetic engineering. In: Brenner's Online Encyclopedia to Genetics, 2nd edn. Elsevier Press.
14. L. Thomason, D.L. Court, and M. Bubunenko Recombineering: Genetic engineering in bacteria using homologous recombination Ch. 1, Unit 16 Current Protocols in Molecular Biology 2007 Wiley Hoboken, NJ 1 24
15. J.A. Sawitzke, L.C. Thomason, N. Costantino, M. Bubunenko, S. Datta, and D.L. Court Recombineering: In vivo genetic engineering in E. coli, S. enterica, and beyond Methods in Enzymology 421 2007 171 199
16. Recombineering: Highly Efficient in vivo genetic engineering using single-strand oligos.
17. Explanatory chapter: PCR -Primer design.
18. Colony PCR.
19. Transformation of E. coli via electroporation.
20. Agarose Gel Electrophoresis.