Recombineering: Highly efficient in vivo genetic engineering using single-strand oligos

Methods in Enzymology

Volumn 533, Issue , 2013, Pages 157-177

  Sawitzke, James A ^a   Thomason, Lynn C ^a   Bubunenko, Mikhail ^a   Li, Xintian ^a   Costantino, Nina ^a   Court, Donald L ^a  

^a NATIONAL CANCER INSTITUTE   (United States)

Author keywords

Bacterial outgrowth; Designing the oligo for recombineering; Double strand DNA (dsDNA); In vivo genetic engineering; Methyl directed mismatch repair (MMR) system; Single strand DNA (ssDNA); Single strand oligos

Indexed keywords

SINGLE STRANDED DNA;

ARTICLE; CONTROLLED STUDY; DNA DETERMINATION; DNA SEQUENCE; ELECTROTRANSFORMATION; GENE INSERTION SEQUENCE; GENETIC ENGINEERING; GENETIC PROCEDURES; IN VIVO STUDY; NONHUMAN; POINT MUTATION; PRIORITY JOURNAL; RECOMBINEERING;

BACTERIA (MICROORGANISMS);

BACTERIAL OUTGROWTH; DESIGNING THE OLIGO FOR RECOMBINEERING; DOUBLE-STRAND DNA (DSDNA); IN VIVO GENETIC ENGINEERING; METHYL-DIRECTED MISMATCH REPAIR (MMR) SYSTEM; SINGLE-STRAND DNA (SSDNA); SINGLE-STRAND OLIGOS;

DNA MISMATCH REPAIR; DNA PRIMERS; DNA, SINGLE-STRANDED; ELECTROPORATION; ESCHERICHIA COLI; GENETIC ENGINEERING; HOMOLOGOUS RECOMBINATION; MUTATION; OLIGONUCLEOTIDES; POINT MUTATION; POLYMERASE CHAIN REACTION;

EID: 84887245041     PISSN: 00766879     EISSN: 15577988     Source Type: Book Series    
DOI: 10.1016/B978-0-12-420067-8.00010-6     Document Type: Chapter

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15. Recombineering: Using Drug Cassettes to Knock out Genes in vivo.
16. Explanatory chapter: PCR -Primer design.
17. Transformation of E. coli via electroporation.
18. Colony PCR.
19. Agarose Gel Electrophoresis.