Biochemical analysis and structure determination of Paucimonas lemoignei poly(3-hydroxybutyrate) (PHB) depolymerase PhaZ7 muteins reveal the PHB binding site and details of substrate-enzyme interactions

Molecular Microbiology

Volumn 90, Issue 3, 2013, Pages 649-664

  Jendrossek, Dieter ^a   Hermawan, Siska ^a   Subedi, Bishwa ^b   Papageorgiou, Anastassios C ^b  

^a UNIVERSITY OF STUTTGART   (Germany)

^b UNIVERSITY OF TURKU   (Finland)

Author keywords

[No Author keywords available]

Indexed keywords

ALANINE; BACTERIAL ENZYME; DEPOLYMERASE PHAZ7; HYDROLASE; POLY(3 HYDROXYBUTYRIC ACID); SERINE; TETRAMER; TYROSINE; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ARTICLE; BACTERIUM; BACTERIUM MUTANT; BINDING SITE; CONFORMATIONAL TRANSITION; CONTROLLED STUDY; CRYSTAL STRUCTURE; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME BINDING; ENZYME STRUCTURE; ENZYME SUBSTRATE COMPLEX; GENE DELETION; GENETIC VARIABILITY; HYDROPHOBICITY; NONHUMAN; PAUCIMONAS LEMOIGNEI; PRIORITY JOURNAL; PROTEIN DETERMINATION; PROTEIN DOMAIN; PROTEIN FOLDING; PROTEIN PURIFICATION; RESIDUE ANALYSIS; SITE DIRECTED MUTAGENESIS;

PAUCIMONAS LEMOIGNEI;

AMINO ACID SUBSTITUTION; BACTERIAL PROTEINS; BURKHOLDERIACEAE; CARBOXYLIC ESTER HYDROLASES; CATALYTIC DOMAIN; HYDROXYBUTYRATES; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; POLYESTERS; PROTEIN CONFORMATION; PROTEIN STRUCTURE, SECONDARY; SEQUENCE DELETION; SEQUENCE HOMOLOGY, AMINO ACID; SUBSTRATE SPECIFICITY; TYROSINE;

EID: 84887100356     PISSN: 0950382X     EISSN: 13652958     Source Type: Journal    
DOI: 10.1111/mmi.12391     Document Type: Article

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