Sample preparation for two-dimensional gel electrophoresis: Considering the composition of biological material

Proteomics

Volumn 13, Issue 21, 2013, Pages 3106-2108

  Knigge, Thomas ^a   Letendre, Julie ^a   Monsinjon, Tiphaine ^a  

^a UNIVERSITÉ DU HAVRE   (France)

Author keywords

Animal proteomics; Ecotoxicology; Nonmodel organisms; Protein extraction; Sample preparation; TRI reagent

Indexed keywords

BIOMATERIAL; RNA;

ECOTOXICOLOGY; GENE EXPRESSION; NOTE; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEOMICS; TWO DIMENSIONAL GEL ELECTROPHORESIS;

ANIMAL PROTEOMICS; ECOTOXICOLOGY; NONMODEL ORGANISMS; PROTEIN EXTRACTION; SAMPLE PREPARATION; TRI-REAGENT;

ANIMALS; ECOTOXICOLOGY; ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; FLOUNDER; GUANIDINES; PHENOLS; POLYCHAETA; PROTEINS; PROTEOMICS; RNA;

EID: 84887070144     PISSN: 16159853     EISSN: 16159861     Source Type: Journal    
DOI: 10.1002/pmic.201300436     Document Type: Note

References (12)

1. Wilkins, M. R., Sanchez, J. C., Gooley, A. A., Appel, R. D. et al., Progress with proteome projects: why all proteins expressed by a genome should be identified and how to do it. Biotechnol. Genet. Eng. Rev. 1996, 13, 19-50.
2. Manduzio, H., Cosette, P., Gricourt, L., Jouenne, T. et al., Proteome modifications of blue mussel (Mytilus edulis L.) gills as an effect of water pollution. Proteomics 2005, 5, 4958-4963.
3. Letendre, J., Dupont-Rouzeyrol, M., Hanquet, A. C., Durand, F., Impact of toxicant exposure on the proteomic response to intertidal condition in Mytilus edulis. Comp. Biochem. Physiol. Part D Genomics Proteomics. 2011, 6, 357-369.
4. Panchout, F., Letendre, J., Bultelle, F., Denier, X. et al., Comparison of protein extraction methods for gills of the shore crab, Carcinus maenas (L.) and application to 2DE. J. Biomol. Tech. 2013, doi: 10.7171/jbt.13-2404-002.
5. Wu, H., Ji, C., Wei, L., Zhao, J., Evaluation of protein extraction protocols for 2-DE in marine ecotoxicoproteomics. Proteomics 2013, 13, 3205-3210.
6. Nielsen, K., Vorum, H., Fagerholm, P., Birkenkamp-Demtröder, K. et al., Proteome profiling of corneal epithelium and identification of marker proteins for keratoconus, a pilot study. Exp. Eye Res. 2006, 82, 201-209.
7. Lee, F. W., Lo, S. C., The use of Trizol reagent (phenol/guanidine isothiocyanate) for producing high quality two-dimensional gel electrophoretograms (2-DE) of dinoflagellates. J. Microbiol. Methods 2008, 73, 26-32.
8. Lyon, B. R., Lee, P. A., Bennett, J. M., DiTullio, G. R. et al., Proteomic analysis of a sea-ice diatom: salinity acclimation provides new insight into the dimethylsulfoniopropionate production pathway. Plant Physiol. 2011, 157, 1926-1941.
9. Elkan-Miller, T., Ulitsky, I., Hertzano, R., Rudnicki, A. et al., Integration of transcriptomics, proteomics, and microRNA analyses reveals novel microRNA regulation of targets in the mammalian inner ear. PLoS One 2011, 6, e18195.
10. Butt, R. H., Pfeifer, T. A., Delaney, A., Grigliatti, T. A. et al., Enabling coupled quantitative genomics and proteomics analyses from rat spinal cord samples. Mol. Cell. Proteomics 2007, 6, 1574-1588.
11. Young, C., Truman, P., Proteins isolated with TRIzol are compatible with two-dimensional electrophoresis and mass spectrometry analyses. Anal. Biochem. 2012, 421, 330-332.
12. Hummon, A. B., Lim, S. R., Difilippantonio, M. J., Ried, T., Isolation and solubilization of proteins after TRIzol extraction of RNA and DNA from patient material following prolonged storage. Biotechniques 2007, 42, 467-472.