메뉴 건너뛰기




Volumn 73, Issue 1, 2008, Pages 26-32

The use of Trizol reagent (phenol/guanidine isothiocyanate) for producing high quality two-dimensional gel electrophoretograms (2-DE) of dinoflagellates

Author keywords

Dinoflagellate; HABs; Proteomics; Trizol; Two dimensional gel electrophoresis

Indexed keywords

GUANIDINE DERIVATIVE; ISOTHIOCYANIC ACID DERIVATIVE; PHENOL; RIBULOSEBISPHOSPHATE CARBOXYLASE; RUBISCO II ENZYME; UNCLASSIFIED DRUG;

EID: 40849120305     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mimet.2008.01.006     Document Type: Article
Times cited : (62)

References (15)
  • 1
    • 0842289038 scopus 로고    scopus 로고
    • Biological rhythmicity in expressed proteins of the marine dinoflagellate Lingulodinium polyedrum demonstrated by chronological proteomics
    • Akimoto H., Wu C., Kinumi T., and Ohmiya Y. Biological rhythmicity in expressed proteins of the marine dinoflagellate Lingulodinium polyedrum demonstrated by chronological proteomics. Biochem. Biophys. Res. Commun. 315 (2004) 306-312
    • (2004) Biochem. Biophys. Res. Commun. , vol.315 , pp. 306-312
    • Akimoto, H.1    Wu, C.2    Kinumi, T.3    Ohmiya, Y.4
  • 2
    • 0036744313 scopus 로고    scopus 로고
    • Proteomic study of a model causative agent of harmful red tide, Prorocentrum triestinum I: optimization of sample preparation methodologies for analyzing with two-dimensional electrophoresis
    • Chan L.L., Lo S.C., and Hodgkiss I.J. Proteomic study of a model causative agent of harmful red tide, Prorocentrum triestinum I: optimization of sample preparation methodologies for analyzing with two-dimensional electrophoresis. Proteomics 2 (2002) 1169-1186
    • (2002) Proteomics , vol.2 , pp. 1169-1186
    • Chan, L.L.1    Lo, S.C.2    Hodgkiss, I.J.3
  • 3
    • 0942297960 scopus 로고    scopus 로고
    • Use of two-dimensional gel electrophoresis proteome reference maps of dinoflagellates for species recognition of causative agents of harmful algal blooms
    • Chan L.L., Hodgkiss I.J., Lu S., and Lo S.C. Use of two-dimensional gel electrophoresis proteome reference maps of dinoflagellates for species recognition of causative agents of harmful algal blooms. Proteomics 4 (2004) 180-192
    • (2004) Proteomics , vol.4 , pp. 180-192
    • Chan, L.L.1    Hodgkiss, I.J.2    Lu, S.3    Lo, S.C.4
  • 4
    • 5644255562 scopus 로고    scopus 로고
    • Proteomic study of a model causative agent of harmful algal blooms, Prorocentrum triestinum II: the use of differentially expressed protein profiles under different growth phases and growth conditions for bloom prediction
    • Chan L.L., Hodgkiss I.J., Wan J.M., Lum J.H., Mak A.S., Sit W.H., and Lo S.C. Proteomic study of a model causative agent of harmful algal blooms, Prorocentrum triestinum II: the use of differentially expressed protein profiles under different growth phases and growth conditions for bloom prediction. Proteomics 4 (2004) 3214-3226
    • (2004) Proteomics , vol.4 , pp. 3214-3226
    • Chan, L.L.1    Hodgkiss, I.J.2    Wan, J.M.3    Lum, J.H.4    Mak, A.S.5    Sit, W.H.6    Lo, S.C.7
  • 5
    • 17844391843 scopus 로고    scopus 로고
    • Use of two-dimensional gel electrophoresis to differentiate morphospecies of Alexandrium minutum, a paralytic shellfish poisoning toxin-producing dinoflagellate of harmful algal blooms
    • Chan L.L., Hodgkiss I.J., Lam P.K., Wan J.M., Chou H.N., Lum J.H., et al. Use of two-dimensional gel electrophoresis to differentiate morphospecies of Alexandrium minutum, a paralytic shellfish poisoning toxin-producing dinoflagellate of harmful algal blooms. Proteomics 5 (2005) 1580-1593
    • (2005) Proteomics , vol.5 , pp. 1580-1593
    • Chan, L.L.1    Hodgkiss, I.J.2    Lam, P.K.3    Wan, J.M.4    Chou, H.N.5    Lum, J.H.6
  • 6
    • 32144460520 scopus 로고    scopus 로고
    • Identification and characterization of a "biomarker of toxicity" from the proteome of the paralytic shellfish toxin-producing dinoflagellate Alexandrium tamarense (Dinophyceae)
    • Chan L.L., Sit W.H., Lam P.K., Hsieh D.P., Hodgkiss I.J., Wan J.M., et al. Identification and characterization of a "biomarker of toxicity" from the proteome of the paralytic shellfish toxin-producing dinoflagellate Alexandrium tamarense (Dinophyceae). Proteomics 6 (2006) 654-666
    • (2006) Proteomics , vol.6 , pp. 654-666
    • Chan, L.L.1    Sit, W.H.2    Lam, P.K.3    Hsieh, D.P.4    Hodgkiss, I.J.5    Wan, J.M.6
  • 9
    • 0033594994 scopus 로고    scopus 로고
    • A method for high-sensitivity peptide sequencing using postsource decay matrix-assisted laser desorption ionization mass spectrometry
    • Keough T., Youngquist R.S., and Lacey M.P. A method for high-sensitivity peptide sequencing using postsource decay matrix-assisted laser desorption ionization mass spectrometry. Proc. Natl. Acad. Sci. U.S.A. 96 (1999) 7131-7136
    • (1999) Proc. Natl. Acad. Sci. U.S.A. , vol.96 , pp. 7131-7136
    • Keough, T.1    Youngquist, R.S.2    Lacey, M.P.3
  • 10
    • 0034534884 scopus 로고    scopus 로고
    • Derivatization procedures to facilitate de novo sequencing of lysine-terminated tryptic peptides using postsource decay matrix-assisted laser desorption/ionization mass spectrometry
    • Keough T., Lacey M.P., and Youngquist R.S. Derivatization procedures to facilitate de novo sequencing of lysine-terminated tryptic peptides using postsource decay matrix-assisted laser desorption/ionization mass spectrometry. Rapid. Commun. Mass. Spectrom. 14 (2000) 2348-2356
    • (2000) Rapid. Commun. Mass. Spectrom. , vol.14 , pp. 2348-2356
    • Keough, T.1    Lacey, M.P.2    Youngquist, R.S.3
  • 11
    • 33645291760 scopus 로고    scopus 로고
    • Trizol-based method for sample preparation and isoelectric focusing of halophilic proteins
    • Kirkland P.A., Busby J., Stevens Jr. S., and Maupin-Furlow J.A. Trizol-based method for sample preparation and isoelectric focusing of halophilic proteins. Anal. Biochem. 351 (2006) 254-259
    • (2006) Anal. Biochem. , vol.351 , pp. 254-259
    • Kirkland, P.A.1    Busby, J.2    Stevens Jr., S.3    Maupin-Furlow, J.A.4
  • 12
    • 35649005478 scopus 로고    scopus 로고
    • Improved protein identification efficiency by mass spectrometry using N-terminal chemical derivatization of peptides from Angiostrongylus costaricensis, a nematode with unknown genome
    • Leon I.R., Neves-Ferreira A.G., Valente R.H., Mota E.M., Lenzi H.L., and Perales J. Improved protein identification efficiency by mass spectrometry using N-terminal chemical derivatization of peptides from Angiostrongylus costaricensis, a nematode with unknown genome. J. Mass. Spectrom. 42 (2007) 1363-1374
    • (2007) J. Mass. Spectrom. , vol.42 , pp. 1363-1374
    • Leon, I.R.1    Neves-Ferreira, A.G.2    Valente, R.H.3    Mota, E.M.4    Lenzi, H.L.5    Perales, J.6
  • 13
    • 0016711037 scopus 로고
    • High resolution two-dimensional electrophoresis of proteins
    • O'Farrell P.H. High resolution two-dimensional electrophoresis of proteins. J. Biol. Chem. 250 (1975) 4007-4021
    • (1975) J. Biol. Chem. , vol.250 , pp. 4007-4021
    • O'Farrell, P.H.1
  • 14
    • 84988119829 scopus 로고
    • Quantitation of microgram amounts of protein in two-dimensional polyacrylamide-gel electrophoresis sample buffer
    • Ramagli L.S., and Rodriguez L.V. Quantitation of microgram amounts of protein in two-dimensional polyacrylamide-gel electrophoresis sample buffer. Electrophoresis 6 (1985) 559-563
    • (1985) Electrophoresis , vol.6 , pp. 559-563
    • Ramagli, L.S.1    Rodriguez, L.V.2
  • 15
    • 0346158179 scopus 로고    scopus 로고
    • Improved procedures for N-terminal sulfonation of peptides for matrix-assisted laser desorption/ionization post-source decay peptide sequencing
    • Wang D., Kalb S.R., and Cotter R.J. Improved procedures for N-terminal sulfonation of peptides for matrix-assisted laser desorption/ionization post-source decay peptide sequencing. Rapid Commun. Mass Spectrom. 18 (2004) 96-102
    • (2004) Rapid Commun. Mass Spectrom. , vol.18 , pp. 96-102
    • Wang, D.1    Kalb, S.R.2    Cotter, R.J.3


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.