Crystal structure of Bacillus subtilis GabR, an autorepressor and transcriptional activator of gabT

Proceedings of the National Academy of Sciences of the United States of America

Volumn 110, Issue 44, 2013, Pages 17820-17825

  Edayathumangalam, Raji ^a,b   Wu, Rui ^c   Garcia, Roman ^c   Wang, Yuguang ^c   Wang, Wei ^d   Kreinbring, Cheryl A ^b   Bach, Alicia ^b   Liao, Jingling ^d   Stone, Todd A ^e   Terwilliger, Thomas C ^f   Hoang, Quyen Q ^d   Belitsky, Boris R ^g   Petsko, Gregory A ^b   Ringe, Dagmar ^b   Liu, Dali ^c  

^a Brigham and Women's Hospital Center for Neurologic Diseases   (United States)

^b BRANDEIS UNIVERSITY   (United States)

^c LOYOLA UNIVERSITY CHICAGO   (United States)

^d INDIANA UNIVERSITY SCHOOL OF MEDICINE   (United States)

^e INDIANA UNIVERSITY   (United States)

^f LOS ALAMOS NATIONAL LABORATORY   (United States)

^g TUFTS UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

4 AMINOBUTYRATE AMINOTRANSFERASE; 4 AMINOBUTYRIC ACID RECEPTOR;

AMINO TERMINAL SEQUENCE; ARTICLE; BACILLUS SUBTILIS; CARBON SOURCE; CARBOXY TERMINAL SEQUENCE; CRYSTAL STRUCTURE; DNA BINDING; ENZYME ACTIVITY; FLUORESCENCE POLARIZATION; PRIORITY JOURNAL; PROTEIN QUATERNARY STRUCTURE;

BACILLUS SUBTILIS; DIMERIZATION; EVOLUTION, MOLECULAR; GAMMA-AMINOBUTYRIC ACID; GENE EXPRESSION REGULATION, BACTERIAL; MODELS, MOLECULAR; PROTEIN CONFORMATION; PYRIDOXAL PHOSPHATE; TRANSCRIPTION FACTORS;

EID: 84887047300     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.1315887110     Document Type: Article

References (19)

1. Rigali S, Derouaux A, Giannotta F, Dusart J (2002) Subdivision of the helix-Turn-helix GntR family of bacterial regulators in the FadR, HutC, MocR, and YtrA subfamilies. J Biol Chem 277(15):12507-12515.
2. Belitsky BR, Sonenshein AL (2002) GabR, a member of a novel protein family, regulates the utilization of gamma-aminobutyrate in Bacillus subtilis. Mol Microbiol 45(2): 569-583.
3. Bramucci E, Milano T, Pascarella S (2011) Genomic distribution and heterogeneity of MocR-like transcriptional factors containing a domain belonging to the superfamily of the pyridoxal-5α-phosphate dependent enzymes of fold type I. Biochem Biophys Res Commun 415(1):88-93.
4. Kirsch JF, et al. (1984) Mechanism of action of aspartate aminotransferase proposed on the basis of its spatial structure. J Mol Biol 174(3):497-525.
5. Schneider G, Käck H, Lindqvist Y (2000) The manifold of vitamin B6 dependent enzymes. Structure 8(1):R1-R6.
6. Belitsky BR (2004) Bacillus subtilis GabR, a protein with DNA-binding and aminotransferase domains, is a PLP-dependent transcriptional regulator. J Mol Biol 340(4): 655-664.
7. Ouchi T, Tomita T, Miyagawa T, Kuzuyama T, Nishiyama M (2009) Dual roles of a conserved pair, Arg23 and Ser20, in recognition of multiple substrates in alphaaminoadipate aminotransferase from Thermus thermophilus. Biochem Biophys Res Commun 388(1):21-27.
8. Gajiwala KS, Burley SK (2000) Winged helix proteins. Curr Opin Struct Biol 10(1): 110-116.
9. Eliot AC, Kirsch JF (2004) Pyridoxal phosphate enzymes: Mechanistic, structural, and evolutionary considerations. Annu Rev Biochem 73:383-415.
10. Smith DL, Almo SC, Toney MD, Ringe D (1989) 2.8-A-resolution crystal structure of an active-site mutant of aspartate aminotransferase from Escherichia coli. Biochemistry 28(20):8161-8167.
11. Marchler-Bauer A, et al. (2011) CDD: A Conserved Domain Database for the functional annotation of proteins. Nucleic Acids Res 39(Database issue):D225-D229.
12. van Aalten DM, DiRusso CC, Knudsen J (2001) The structural basis of acyl coenzyme A-dependent regulation of the transcription factor FadR. EMBO J 20(8):2041-2050.
13. Raman N, Black PN, DiRusso CC (1997) Characterization of the fatty acid-responsive transcription factor FadR. Biochemical and genetic analyses of the native conformation and functional domains. J Biol Chem 272(49):30645-30650.
14. Storici P, et al. (2004) Structures of gamma-aminobutyric acid (GABA) aminotransferase, a pyridoxal 5α-phosphate, and [2Fe-2S] cluster-containing enzyme, complexed with gamma-ethynyl-GABA and with the antiepilepsy drug vigabatrin. J Biol Chem 279(1):363-373.
15. Hirotsu K, Goto M, Okamoto A, Miyahara I (2005) Dual substrate recognition of aminotransferases. Chem Rec 5(3):160-172.
16. Grose JH, Bergthorsson U, Roth JR (2005) Regulation of NAD synthesis by the trifunctional NadR protein of Salmonella enterica. J Bacteriol 187(8):2774-2782.
17. Tomchick DR, Turner RJ, Switzer RL, Smith JL (1998) Adaptation of an enzyme to regulatory function: Structure of Bacillus subtilis PyrR, a pyr RNA-binding attenuation protein and uracil phosphoribosyltransferase. Structure 6(3):337-350.
18. Sinha SC, et al. (2003) The purine repressor of Bacillus subtilis: A novel combination of domains adapted for transcription regulation. J Bacteriol 185(14):4087-4098.
19. Jain D, et al. (2005) Crystal structure of bacteriophage lambda cII and its DNA complex. Mol Cell 19(2):259-269.