Novel Sulfated Polysaccharides of Red Microalgae: Basics and Applications

Handbook of Microalgal Culture: Applied Phycology and Biotechnology: Second Edition

Volumn , Issue , 2013, Pages 406-416

  Arad, Shoshana Malis ^a   van Moppes, Dorit ^a  

^a BEN GURION UNIVERSITY OF THE NEGEV   (Israel)

Author keywords

algae products; cell wall polysaccharides; microalgae cultivation; red microalgae

Indexed keywords

MICROORGANISMS; POLYSACCHARIDES;

CELL WALL POLYSACCHARIDES; CHEMICAL CHARACTERIZATION; LARGE SCALE CULTIVATIONS; MICRO-ALGAE; MICROALGAE CULTIVATION; PHARMACEUTICAL APPLICATIONS; PROTEIN PRODUCTION; SULFATED POLYSACCHARIDES;

ALGAE;

EID: 84886980510     PISSN: None     EISSN: None     Source Type: Book    
DOI: 10.1002/9781118567166.ch21     Document Type: Chapter

References (106)

1. Adda, M., Merchuk, J. & Arad, S. (1985) The potential of production of sulfated polysaccharides from Porphyridium. Plant Soil 89: 117-127.
2. Adda, M., Merchuk, J.C. & Arad (Malis), S. (1986) Effect of nitrate on growth and production of cell-wall polysaccharide by the unicellular red alga. Porphyridium Biomass 10: 131-140.
3. Apt, K.E., Grossman, A.R. & Kroth-Pancic, P.G. (1996) Stable nuclear transformation of the diatom Phaeodactylum tricornutum. Mol. Gen. Genet. 252: 572-579.
4. Arad (Malis), S. (1988) Production of sulfated polysaccharides from red unicellular algae. In: Algal Biotechnology (eds T. Stadler, J. Mollion & M.C.Verduset), pp. 65-87 Elsevier Applied Science, London.
5. Arad (Malis), S. (1999) Polysaccharides of red microalgae. In: Chemicals from Microalgae (ed. Z. Cohen), pp. 282-287. Taylor & Francis, London.
6. Arad (Malis), S. & Yaron, A. (1992) Natural pigments from red microalgae for use in foods and cosmetics. Trends Food Sci. Technol. 4(3): 92-97.
7. Arad, S. & Weinstein, Y. (2003) Novel lubricants from red microalgae: interplay between genes and products. Biomedic (Israel) 1: 32-37.
8. Arad, S. & Richmond, A. (2004) Industrial production of microalgal cell-mass and secondary products-species of high potential: Porphyridium sp. In: Handbook ofMicroal-gal Culture: Biotechnology & Applied Phycology (ed. A. Richmond), pp. 289-297. Blackwell Science, Carlton, Australia.
9. Arad (Malis), S. & Levy-Ontman, O. (2010) Red microalgal cell-wall polysaccharides: biotechnological aspects. Curr. Opin. Biotechnol. 21: 358-364.
10. Arad (Malis), S. & Levy-Ontman, O. (2012) Sulfated polysaccharides in the cell wall of red microalgae: structure, properties and applications. In: Biopolymer-BasedMaterials: From Blends and Composites to Gels and Complex Networks (eds T. Sabu, D. Dominique, C. Christophe & P. Jyotishkumar), John Wiley & Sons, Weinheim, Germany.
11. Arad (Malis), S., Friedman (Dahan), O. & Rotem, A. (1988) Effect of nitrogen on polysaccharide production in a Porphyridium sp. Appl. Environ. Microbiol. 54(10): 2411-2414.
12. Arad (Malis), S., Lerental (Brown), Y. & Dubinsky, O. (1992) Effect of nitrate and sulfate starvation on polysaccharide formation in Rhodella reticulata. Bioresour. Technol. 42: 141-148.
13. Arad (Malis), S., Dubinsky, O. & Simon, B. (1993a) A modified cell wall mutant of the red microalga Rhodella reticulata resistant to the herbicide 2,6-dichlorobenzonitrile. J. Phycol. 29: 309-313.
14. Arad (Malis), S., Keristovsky, G., Simon, B., Barak, Z. & Geresh, S. (1993b) Biodegradation of the sulfated polysaccharide of Porphyridium by soil bacteria. Phytochemistry 32(20): 287-290.
15. Arad (Malis), S., Kolani, R., Simon-Berkovitch, B. & Sivan, A. (1994) Inhibition by DCB of cell-wall polysaccharide formation intheredmicroalgaPorphyridium sp. (Rhodophyta). Phycologia 33: 158-162.
16. Arad (Malis), S., Ginzberg, A. & Huleihel, M. (2006a) Antiviral activity of sulfated polysaccharides of marine red algae. In: Recent Advances in Marine Biotechnology: Biomaterials from Aquatic and Terrestrial Organisms. pp. 37-62. Science Publishers Inc., Enfield, NH.
17. Arad (Malis), S., Rapoport, L., Moshkovich, A., van Moppes, D., Karpasas, M., Golan, R. & Golan, Y. (2006b) A superior biolubricant from a species of red microalga. Langmuir 22: 7313-7317.
18. Capek, P., Matulova, M. & Combourieu, B. (2008) The extracellular proteoglycan produced by Rhodella grisea. Int. J. Biol. Macromol. 43: 390-393.
19. Chaumont, D., Thepenier, C., Gudin, C. & Junjas, C. (1988) Scaling up a tubular photoreactor for continuous culture of Porphyridium cruentum from laboratory to pilot plant (1981-1987). In: Algal Biotechnology (eds T. Stadler, J. Mollion, M.C. Verdus, Y. Karamanos, H. Morvan & D. Christiaen), pp. 199-208. Elsevier Applied Science, London.
20. Cheng, R., Ma, R., Rong, H., Lin, X., Wang, Z. & Ma, Y. (2012) Agrobacterium tumefaciens mediated transformation of marine microalgae Schizochytrium. Microbiol. Res. 167(3): 179-186.
21. Cohen, E. & Arad (Malis), S. (1989) A closed system for outdoor cultivation of Porphyridium. Biomass 18: 59-67.
22. Cohen, E., Koren, A. & Arad (Malis), S. (1991) A closed system for outdoor cultivation of microalgae. Biomass Bioenergy 1(2): 83-88.
23. Cohen-Katz, A. & Arad (Malis), S. (1998) Biosynthesis of the cell-wall polysaccharide in the red microalga Rhodella reticulata. Israel J. Botany 46: 147-153.
24. Crawford, N.M., Kahn, M.L., Leustek, T. & Long, S.R. (2000) Nitrogen and sulphur. In: Biochemistry and Molecular Biology of Plants (eds B, Buchanan, W, Gruissen & R, Jones), pp. 786-849. American Society of Plant Physiologists, Rockville, MD.
25. Dubinsky, O., Simon, B., Karamanos, Y., Geresh, S., Barak, Z. & Arad (Malis), S. (1992) Composition of the cell wall polysaccharide produced by the unicellular red alga Rhodella reticulata. Plant Physiol. Biochem. 30(4): 409-414.
26. Dvir, I., Geva, O., Chayoth, R., Sod-Moriah, U., Shany, S., Arad, S. & Madar, Z. (1996) Polysaccharides and algal biomass as new sources of dietary fibers and their physiological effects in rats. FASEB J. 10: 3012.
27. Dvir, I., Chayoth, R., Sod-Moriah, U., Shany, S., Nyska, A., Stark, AH., Madar, Z. & Arad (Malis), S. (2000) Soluble polysaccharide and biomass of red microalga Porphyridium sp. alter intestinal morphology and reduce serum cholesterol in rats. Br J. Nutr. 84: 469-476.
28. Dvir, I., Stark, A.H., Chayoth, R., Madar, Z. & Arad (Malis), S. (2009) Hypocholesterolemic effects of nutraceuticals produced from the red microalga Porphyridium sp. Nutrients 1: 156-167.
29. Eteshola, E., Gottlieb, M. & Arad (Malis), S. (1996) Dilute solution viscosity of red microalga exopolysaccharide. Chem. Eng. Sci. 51(9): 1487-1494
30. Eteshola, E., Karpasas, M., Arad (Malis), S. & Gottlieb, M. (1998) Red microalga exopolysaccharides: 2. Study of the rheology, morphology and thermal gelation of aqueous preparations. Acta. Polym. 49: 549-556.
31. Evans, L., Callow, M., Percival, E. & Fareed, V. (1974) Studies on the synthesis and composition of extracellular mucilage in the unicellular red alga. Rhodella J. Cell. Sci. 16: 1-21.
32. Fabregas, J., Garcia, D., Fernandez-Alonso, M., Rocha, A.I., Gomez-Puertas, P., Escribano, J.M., Otero, A. & Coll, J.M. (1999) In vitro inhibition of the replication of haemorrhagic septicaemia virus (VHSV) and African swine fever virus (ASFV) by extracts from marine microalgae. Antiviral Res. 44: 67-73.
33. Falciatore, A., Casotti, R., Leblanc, C., Abrescia, C. & Bowler, C. (1999) Transformation of nonselectable reporter genes in marine diatoms. Mar. Biotechnol. 1: 239-251.
34. Flaibani, A., Olsen, Y. & Painter, T.J. (1989). Polysaccharides in desert reclamation: compositions of exocellular proteoglycan complexes produced by filamentous blue-green and unicellular green edaphic algae. Carbohydr Res. 190: 235-248.
35. Fletcher, S.P., Muto, M. & Mayfield, S. (2007) Optimization of recombinant protein expression in the chloroplasts of green algae. In: Transgenic Microalgae as Green Cell Factories. Vol. 616 (eds R. Leon, A. Gavan & E. Fernandez), pp. 90-98. Springer, New York.
36. Friedman, O., Dubinsky, Z. & Arad (Malis), S. (1991) Effect of light intensity on growth and polysaccharide production in red and blue-green Rhodophyta unicells. Bioresour. Technol. 38: 105-110.
37. Galvan, A., Gozalez-Ballester, D. & Fernandez, E. (2007) Insertional mutagenesis as tool to study genes/functions in Chlamydomonas. In: Transgenic Microalgae as Green Cell Factories. Vol. 616 (eds R. Leon, A. Galvan & E. Fernandez), pp. 77-89. Springer, New York.
38. Gantt, E. (1981) Phycobilisomes. Annu. Rev. Plant Physiol. 32: 327-347.
39. Gao, Y., Schofield, O. & Leustek, T. (2000) Characterization of sulfate assimilation in marine algae focusing on the enzyme 5'-adenylysulfate reductase. Plant Physiol. 123(3): 1087-1096.
40. Geresh, S. & Arad (Malis), S. (1991) The extracellular polysaccharides of the red microalgae: chemistry and rheology. Bioresour. Technol. 38: 195-201.
41. Geresh, S., Dubinsky, O., Arad (Malis), S., Christi-aen, D. & Glaser, R. (1990) Structure of 3-0-(alpha-D-glucopyranosyluronic acid)-L-galactopyranose, an aldobi-ouronic acid isolated from the polysaccharides of various unicellular red algae. Carbohydr. Res. 208: 301-305.
42. Geresh, S., Adin, I., Yarmolinsky, E. & Karpasas, M. (1992) Characterization of the extracellular polysaccharide of Porphyridium sp.: molecular weight determination and rheological properties. Carbohydr. Polym. 50: 183-189.
43. Geresh, S., Arad (Malis), S. & Shefer, A. (1997) Chemically cross-linked polysaccharide of the red microalga Rhodella reticulata -an ion exchanger for toxic metal ions. J. Carbohydr. Chem. 16(4-5): 703-708.
44. Geresh, S., (Malis) Arad, S., Levy-Ontman, O., Zhang, W., Tekoah, Y. & Glaser, R. (2009) Isolation and characterization of poly-and oligosaccharides from the red microalga Porphyridium sp. Carbohydr. Res. 344: 344-349.
45. Ginzberg, A., Korin, E. & Arad (Malis), S. (2008) Effect of drying on the biological activities of a red microalgal polysaccharide. Biotechnol. Bioeng. 99(2): 411-420.
46. Gong, Y., Hu, H., Gao, Y., Xu, X. & Gao, H. (2011) Microalgae as platforms for production of recombinant proteins and valuable compounds: progress and prospects. J. Ind. Microbiol. Biotechnol. 38: 1879-1890.
47. Gourdon, D., Lin, Q., Oroudjev, E., Hansma, H., Golan, Y., Arad, S. & Israelachvili, J. (2008) Adhesion and stable low friction provided by a subnanometer-thick monolayer of a natural polysaccharide. Langmuir 24(4): 1534-1540.
48. Griesbeck, C., Kobl, I. & Heitzer, M. (2006) Chlamydomonas reinhardtii: a protein expression system for pharmaceutical and biotechnological proteins. Mol. Biotechnol. 34: 213- 223.
49. Gruber, A., Vugrinec, S., Hempel, F., Gould, S., Maier, U.-G. & Kroth, P. (2007) Protein targeting into complex diatom plastids: functional characterization of a specific targeting motif. Plant Mol. Biol. 64: 519-530.
50. Gudin, C. & Chaumont, D. (1991) Cell fragility -the key problem of microalgae mass production in closed photobioreactors. Bioresour. Technol. 38: 145-151.
51. Hallman, A. (2007) Algal transgenic and biotechnology. Transgenic Plant J. 1(1): 89-98.
52. Heaney-Kieras, J. & Chapman, DJ. (1976) Structural studies on the extracellular polysaccharide of the red alga Porphyridium cruentum. Carbohydr. Res. 52: 169-177.
53. Huleihel, M. & Arad, S. (2001) Effect of Porphyridium sp. polysaccharide on malignant cell transformation by Moloney murine sarcoma virus. Anticancer Res. 21: 2073-2078.
54. Huleihel, M., Ishanu, V., Tal, J. & Arad (Malis), S. (2001) Antiviral effect of red microalgal polysaccharide on Herpes simplex and Varicella zoster viruses. J. Appl. Phycol. 13: 127-134.
55. Huleihel, M., Ishanu, V., Tal, J. & Arad (Malis), S. (2002) Activity of Porphyridium sp. polysaccharide against herpes simplex viruses in vitro and in vivo. J. Biochem. Bioph. Methods 50: 189-200.
56. Jaseja, M., Perlin, A.S., Dubinsky, O., Christiaen, D., (Malis) Arad, S. & Glaser, R. (1989) NMR structure determination of 3-0-(alpha-D-glucopyranosyluronic acid)-L-galactopyranose, an aldobiuronic acid isolated from the unicellular red alga Rhodella reticulata. Carbohydr. Res. 186: 313-319.
57. Keidan, M., Broshy, H., van Moppes, D. & Arad (Malis), S. (2006) Assimilation of sulfur into the cell-wall polysaccharide of the red microalga Porphyridium sp. (Rhodophyta). Phycologia 45(4): 505-511.
58. Keidan, M., Friedlander, M. & Arad (Malis), S. (2009) Effect of Brefeldin A on cell-wall polysaccharide production in the red microalga Porphyridium sp. (Rhodophyta) through its effect on the golgi apparatus. J. Appl. Phycol. 21: 707-717.
59. Kost, H.P., Senser, M. & Wanner, G. (1984) Effect of nitrate and sulfate starvation on Porphyridium cruentum cells. Z. Pflanzenphysiol. 1135: 231-249.
60. Kroen, W.K. & Ramus, J. (1990) Viscous polysaccharide and starch synthesis in Rhodella reticulata (Porphyridiales, Rhodophyta). J. Phycol. 26: 226-271.
61. Levy-Ontman, O., (Malis) Arad, S., Harvey, D.S.J., Parsons, T.B., Fairbanks, A. & Tekoah, Y. (2011) Unique A-glycan moieties of the 66-kDa cell wall glycoprotein from the red microalga Porphyridium sp. J. Biol. Chem. 286(24): 21340-21352.
62. Lapidot, M., Raveh, D., Sivan, A., Arad (Malis), S. & Shapira, M. (1999) Molecular analysis of the AHAS gene of Porphyridium sp. (Rhodophyta) and of a mutant resistant to sulfometuron methyl. J. Phycol. 35: 1233-1236.
63. Lapidot, M., Raveh, D., Sivan, A., Arad, S.M. & Shapira, M. (2002) Stable chloroplast transformation of the unicellular red alga Porphyridium sp. Plant Physiol. 129: 7-12.
64. Lapidot, M., Shrestha, R.P., Weinstein, Y. & Arad (Malis), S. (2010) Red microalgae: from basic know-how to biotechnology. In: Red Algae in the Genomic Age (eds S. Joseph & C. David), Cellular Origin, Life in Extreme Habitats and Astrobiology, Vol. 13, pp. 205-225. Springer, Dordrecht, The Netherlands.
65. Li, S.-Y., Shabtai, Y. & Arad (Malis), S. (2000) Production and composition of the sulphated cell-wall polysaccharide of Porphyridium sp. (Rhodophyta) as affected by CO2 concentration. Phycologia 39: 332-336.
66. Li, S.-Y., Lellouche, J.-P., Shabtai, Y. & Arad (Malis), S. (2001) Fixed carbon partitioning in the red microalga Por-phyridium sp. (Rhodophyta). J. Phycol. 37(2): 289-297.
67. Li, S.-Y., Shabtai, Y. & Arad (Malis), S. (2002) Floridoside as a carbon precursor for the synthesis of cell-wall polysaccharide in the red microalga Porphyridium sp. (Rhodophyta). J. Phycol. 38(5): 931-938.
68. Lupescu, N., Solo-Kwan, J., Christiaen, D., Morvan, H. & Arad (Malis), S. (1992) Structural determination by means of gas-chromatography-mass spectrometry of 3-0-(a-D-glucopyranosyluronic acid)-galactopyranose, an aldobiuronic acid derived from Porphyridium sp. polysaccharide. Carbohydr. Polym. 2: 131-134.
69. Matsui, M.S., Muizzuddin, N., Arad, S. & Marenus, K. (2003) Sulfated polysaccharides from red microalgae have anti-inflammatory properties in vitro and in vivo. Appl. Biochem. Biotechnol. 104: 13-22.
70. Merchuk, J.C., Ronen, M., Giris, S. & Arad (Malis), S. (1998) Light/dark cycles in the growth of the red microalga Porphyridium sp. Biotechnol. Bioeng. 59: 705-713.
71. Minkova, K., Michailov, Y., Toncheva-Panova, T. & Houbavenska, N. (1996) Antiviral activity of Porphyrid-ium cruentum polysaccharide. Pharmazie 51: 3-7.
72. Minoda, A., Sakagami, R., Yagisawa, F., Kuroiwa, T. & Tanaka, K. (2004) Improvement of culture conditions and evidence for nuclear transformation by homologous recombination in a red alga, Cyanidioschyzon merolae 10D. Plant Cell Physiol. 45: 667-671.
73. Painter, T.J. (1993) Carbohydrate polymers in desert reclamation: the potential of microalgal biofertilizers. Carbohydr. Polym. 20: 77-86.
74. Percival, E.P. & Foyle, R.A.J. (1979) The extracellular polysaccharides of Porphyridium cruentum and Porphyridium aerugineum. Carbohydr. Res. 72: 165-176.
75. Plesser, L. (2004) Development of a nuclear transformation system for the red microalga Porphyridium sp. MSc thesis, Ben Gurion University of the Negev, Beer-Sheva, Israel.
76. Plesser, L. (2009) Molecular characterization of the sulfo-transferase from the red microalga Porphyridium sp. PhD thesis, Ben-Gurion University of the Negev, Israel.
77. Plesser, L., Lapidot, M., Weinstein, Y. & Arad (Malis), S. (2007) Sulfotransferase of red microalgae: a molecular and biochemical study. Poster presented at the 8th Marine Biotechnology Conference, March 2007, Eilat, Israel.
78. Purton, S. (2007) Tools and techniques for chloroplast transformation of Chlamydomonas. In: Transgenic Microalgae as Green Cell Factories (eds R. Leon, A. Gavan & E. Fernandez), Vol. 616, pp. 34-45. Springer, New York.
79. Raghavendran, HR. & Srinivasan, P. (2008) Effect of crude sulphated polysaccharide from brown algae against acetaminophen-induced toxicity in rats. Can. J. Physiol. Pharmacol. 86(10): 660-666.
80. Ramus, J. (1972) The production of extracellular polysaccharide by the unicellular red alga Porphyridium aerugineum. J. Phycol. 15: 97-111.
81. Ramus, J. (1986) Rhodophytes unicells: biopolymer physiology and production. In: Algal Biomass Technology (eds W.R. Barclay & R.P. McIntosh), pp. 51-55. J. Cramer, Berlin.
82. Ramus, J. & Kenney, B.E. (1989) Shear degradation as a probe of microalgal exopolymer structure and rheological properties. Biotechnol. Bioeng. 34: 1203-1208.
83. Ramus, J., Kenney, B.E. & Shaughnessy, E.J. (1989) Drag reducing properties of microalgal exopolymers. Biotechnol. Bioeng. 33: 550-557.
84. Savins, J.G. (1978) Oil recovery process employing thickened aqueous driving fluid. Mobil Oil Corporation, NY, U.S. Patent 4,079,544.
85. Shrestha, R.P., Weinstein, Y., Bar-Zvi, D. & Arad, S.M. (2004) A glycoprotein noncovalently associated with cell-wall polysaccharide of the red microalga Porphyridium sp. (Rhodophyta). J. Phycol. 40: 568-580.
86. Simon-Bercovitch, B. (1997) Cell-wall formation in the red microalga Porphyridium sp. PhD thesis, Ben-Gurion University of the Negev, Israel.
87. Simon-Bercovitch, B., Bar-Zvi, D. & Arad (Malis), S. (1999) Cell-wall formation during the cell cycle of Porphyridium sp. (Rhodophyta). J. Phycol. 35: 78-83.
88. Singh, S., Arad, S. & Richmond, A. (2000) Extracellular polysaccharide production in outdoor mass cultures of Porphyridium sp. in flat plate glass reactors. J. Appl. Phycol. 12: 269-275.
89. Sivan, A. & Arad (Malis), S. (1993) Induction and characterization of pigment mutants in the red microalga Porphyrid-ium sp. (Rhodophyceae). Phycologia 32: 68-72.
90. Sivan, A. & Arad (Malis), S. (1995) A mutant of the red microalga Porphyridium sp. (Rhodophyceae) resistant to DCMU and atrazine. Phycologia 34: 299-305.
91. Sivan, A. & Arad (Malis), S. (1998) Intraspecific transfer of herbicide resistance in the red microalga Porphyridium sp. (Rhodophyceae) via protoplast fusion. J. Phycol. 34: 706-711.
92. Sivan A., vanMoppes, D. & Arad (Malis), S. (1995) Protoplast fusion and genetic complementation of pigment mutations in the red microalga Porphyridium sp. J. Phycol. 31: 167-172.
93. Southgate, D.A.T. (1990) Dietary Fiber: Chemical and Biological Aspects (eds D.A.T. Southgate, K. Waldron, I.T. Johnson & G.R. Fenwick), pp. 10-19. The Royal Society of Chemistry, Cambridge.
94. Specht, E., Miyake-Stoner, S. & MayWeld, S.P. (2010) Micro-algae come of age as a platform for recombinant protein production. Biotechnol. Lett. 32:1373-1383.
95. Talyshinsky, M., Souprun, Y. & Huleihel, M. (2002) Antiviral activity of red microalgal polysaccharides against retroviruses. Cancer Cell. Int. 2: 1-8.
96. Tannin-Spitz, T., Bergman, M., van-Moppes, D., Grossman, S. & Arad Malis, S. (2005) Antioxidant activity of the polysaccharide of the red microalga Porphyridium sp. J. Appl. Phycol. 17: 215-222.
97. Thepenier, C., Gudin, C. & Thomas, D. (1985) Immobilization of Porphyridium cruentum in polyurethane foam for the production of polysaccharide. Biomass 7: 225-240.
98. Ucko, M., Cohen, E., Gordin, H. & Arad, S. (1989) Relationship between the unicellular red alga Porphyridium sp. and its predator, the dinoflagellate Gymnodinium sp. Appl. Environ. Microbiol. 55: 2990-2994.
99. Ucko, M., Geresh, S., Simon-Berkovitch, B. & Arad (Malis), S. (1994) Predation by a dinoflagellate on a red microalga with a cell wall modified by sulfate and nitrate starvation. Mar. Ecol. Prog. Ser. 104: 293-298.
100. Ucko, M., Shrestha, R., Mesika, P., Bar-Zvi, D. & Arad Malis, S. (1999) Glycoprotein moiety in the cell wall of the red microalga Porphyridium sp. (Rhodophyta) as the biorecognition site for the Crypthecodinium cohnii-like dinoflagellate. J. Phycol. 35: 1276-1281.
101. van Moppes, D., Barak, Z., Chipman, D.M., Gollop, N. & Arad (Malis), S. (1989) An herbicide (sulfometuron methyl) resistant mutant in Porphyridium (Rhodophyta). J. Phycol. 25: 108-112.
102. Wanner, G. & Kost, H.P. (1984) "Membrane storage" of the red alga Porphyridium cruentum during nitrate and sulphate starvation. Z. Planzenphysiol. 1135: 251-262.
103. Wasowicz, W., Neve, J. & Peretz, A. (1993) Optimized steps in fluorometric determination of thiobarbituric acid-reactive substances in serum: importance of extraction pH and influence of sample preservation and storage. Clin. Chem. 39: 2522-2526.
104. Wolf, S.P. (1994) Ferrous ion oxidation in presence of ferric ion indicator xylenol orange for measurement of hydroperoxides. In: Methods in Enzymology (ed. L.Packer), pp. 182-189. Academic Press.
105. Yaron, A., Dvir, I., Maislos, M., Mokady, S. & Arad Malis, S. (1995) The red microalga Rhodella reticulata as a source of a dietary æ>-3 highly unsaturated fatty acid eicosapen-taenoic acid. In: Food Flavors: Generation, Analysis and Process Influence (ed. G., Charalambous), pp. 665-674. Elsevier Science, Amsterdam.
106. Zaslavskaia, L.A., Lippmeier, J.C., Kroth, P.G., Grossman, A.R. & Apt, K.E. (2000) Transformation of the diatom Phaeodactylum tricornutum (Bacillariophyceae) with a variety of selectable marker and reporter genes. J. Phycol. 36: 379-386.