Chemically crosslinked polysaccharide of the red microalga Rhodella reticulata - An ion exchanger for toxic metal ions

Journal of Carbohydrate Chemistry

Volumn 16, Issue 4-5, 1997, Pages 703-708

  Geresh, Shimona ^a   Arad, Shoshana ^a   Shefer, Adi ^b  

^a BEN GURION UNIVERSITY OF THE NEGEV   (Israel)

^b Department of Electrical Engineering and Computer Science   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

RHODELLA RETICULATA;

EID: 0031491983     PISSN: 07328303     EISSN: None     Source Type: Journal    
DOI: 10.1080/07328309708007349     Document Type: Article

References (12)

1. Presented at the XVIIIth International Carbohydrate Symposium, Milan, Italy, July 21-26, 1996.
2. S. Geresh and S. Arad (Malis), Bioresour. Technol., 38, 195 (1991).
3. N. Lupescu, S. Geresh, S. Arad (Malis), M. Berenstein and R. Glaser, Carbohydr. Res., 210, 3 (1991).
4. S. Geresh, O. Dubinsky, R. Glaser, M. Berenstein, D. Christiaen and S. Arad (Malis), Carbohydr. Res., 208, 301 (1991).
5. O. Dubinsky, B. Simon, Y. Karamanos, S. Geresh, Z. Barak and S. Arad (Malis), Plant Physiol. Biochem., 30(4), 409 (1992).
6. O. Dubinsky, Y. (Brown) Lerental, D. Christiaen, R. Glaser, Z. Barak and S. Arad (Malis) in Algal Biotechnology; T. Stadler, J. Mollion, M.-C. Verdus, Y. Karamanos, H. Morvan and D. Christiaen, Eds.; Elsevier Applied Science, London, 1988, p 451.
7. I.G.F. Gilbert and N.A. Myers, Biochim. Biophys. Acta, 42, 469 (1960).
8. J. R. Dunstone, Biochem. J. 85, 336 (1962).
9. A. Haug and O. Smidrød, Acta Chem. Scand., 24, 843 (1970).
10. 13C shifts were calibrated by substitution using hexamethylbenzene.
11. M. Yalpani in Studies in Organic Chemistry, No. 36: Polysaccharides: Syntheses, Modifications and Structure/Property Relations, Elsevier Science Publishers, Amsterdam, 1988.
12. Polysaccharide suspended in water (7 ml/g dry polysaccharide) was gelatinized by addition, with continuous mixing, of a 5 M sodium hydroxide solution (3.5 ml/g polysaccharide), followed by cross-linking wih epichlorohydrin. The viscous paste was stirred continuously until a gel was formed. The gel was kept as described in Table 1, after which it was crushed and ground into small pieces. A solution of acetic acid (7% w/v) was then added until the pH of the mixture reached pH 5.0. Thereafter, the mixture was homogenized and washed with ethanol/water (1:1 v/v). The gel/water mixture was centrifuged at 5000 rpm for 10 min, and the gel was collected, pressed between two pieces of filter paper and weighed (wet weight). The gel was then lyophilized and weighed again (dry weight).