Global protein quantification of mouse heart tissue based on the silac mouse

Methods in Molecular Biology

Volumn 1005, Issue , 2013, Pages 39-52

  Konzer, Anne ^a   Ruhs, Aaron ^a   Braun, Thomas ^a   Krüger, Marcus ^a  

^a MAX PLANCK INSTITUTE FOR HEART AND LUNG RESEARCH   (Germany)

Author keywords

Cardiomyocytes proteome; Metabolic labeling; SILAC; SILAC mouse; Parvin

Indexed keywords

LYSINE; LYSINE 12 C6; LYSINE 13 C6; UNCLASSIFIED DRUG; ACTIN BINDING PROTEIN; ACTININ; CARBON; PARVA PROTEIN, MOUSE; PARVB PROTEIN, MOUSE; PROTEOME;

ANALYTIC METHOD; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; CELL CULTURE; CELL IMMORTALIZATION; CELL ISOLATION; CELL LABELING; HEART; HEART MUSCLE CELL; MASS SPECTROMETRY; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN ISOLATION; PROTEOMICS; QUANTITATIVE ANALYSIS; SPIKE; STABLE ISOTOPE LABELING WITH AMINO ACID IN CELL CULTURE; UPREGULATION; ANIMAL; CHEMISTRY; CYTOLOGY; GENE EXPRESSION PROFILING; GENE EXPRESSION REGULATION; GENETICS; HEART MUSCLE; ISOTOPE LABELING; KNOCKOUT MOUSE; METABOLISM; METHODOLOGY;

ACTININ; ANIMALS; CARBON ISOTOPES; GENE EXPRESSION PROFILING; GENE EXPRESSION REGULATION; ISOTOPE LABELING; LYSINE; MASS SPECTROMETRY; MICE; MICE, KNOCKOUT; MICROFILAMENT PROTEINS; MYOCARDIUM; MYOCYTES, CARDIAC; PROTEOME;

EID: 84886906404     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-386-2_4     Document Type: Article

References (24)

1. Mathers CD, Loncar D (2006) Projections of global mortality and burden of disease from 2002 to 2030. PLoS Med 3:e442
2. Nagaraj N et al (2011) Deep proteome and transcriptome mapping of a human cancer cell line. Mol Syst Biol 7:548
3. Ong SE, Mann M (2005) Mass spectrometrybased proteomics turns quantitative. Nat Chem Biol 1:252-262
4. Ong SE et al (2002) Stable isotope labeling by amino acids in cell culture, SILAC, as a simple and accurate approach to expression proteomics.Mol Cell Proteomics 1:376-386
5. SoufiB et al (2010) Stable isotope labeling by amino acids in cell culture (SILAC) applied to quantitative proteomics of Bacillus subtilis .J Proteome Res 9:3638-3646
6. Gruhler A et al (2005) Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway. Mol Cell Proteomics 4:310-327
7. Larance M et al (2011) Stable-isotope labeling with amino acids in nematodes. Nat Methods 8:849-851
8. Sury MD, Chen JX, Selbach M (2010) The SILAC fly allows for accurate protein quantification in vivo. Mol Cell Proteomics 9:2173-2183
9. McClatchy DB, Yates JR, III (2008) Stable Isotope Labeling of Mammals (SILAM). CSH Protoc pdb prot4940
10. Kruger M et al (2008) SILAC mouse for quantitative proteomics uncovers kindlin-3 as an essential factor for red blood cell function. Cell134:353-364
11. Waterlow JC (1995) Whole-body protein turnover in humans - past, present, and future. Annu Rev Nutr 15:57-92
12. Schwanhausser B et al (2009) Global analysis of cellular protein translation by pulsed SILAC. Proteomics 9:205-209
13. Doherty MK et al (2005) Proteome dynamics in complex organisms: using stable isotopes to monitor individual protein turnover rates.Proteomics 5:522-533
14. Ong SE, Mann M (2006) A practical recipe for stable isotope labeling by amino acids in cell culture (SILAC). Nat Protoc 1:2650-2660
15. Walther DM, Mann M (2011) Accurate quantification of more than 4000 mouse tissue proteins reveals minimal proteome changes during aging. Mol Cell Proteomics 10(M110): 004523
16. Scholten A et al (2011) In-depth quantitative cardiac proteomics combining electron transfer dissociation and the metalloendopeptidaseLys-N with the SILAC mouse. Mol Cell Proteomics 10(O111):008474
17. Jordan SD et al (2011) Obesity-induced overexpression of miRNA-143 inhibits insulinstimulated AKT activation and impairs glucose metabolism. Nat Cell Biol 13:434- 446
18. Drexler HC et al (2011) On marathons and sprints: an integrated quantitative proteomics and transcriptomics analysis of differences between slow and fast musclefibers. Mol Cell Proteomics 11:M111.010801
19. O'Connell TD, Rodrigo MC, Simpson PC (2007) Isolation and culture of adult mouse cardiac myocytes. Methods Mol Biol 357:271-296
20. Shevchenko A et al (2006) In-gel digestion for mass spectrometric characterization of proteins and proteomes. Nat Protoc 1:2856-2860
21. Rappsilber J, Ishihama Y, Mann M (2003) Stop and go extraction tips for matrix- assisted laser desorption/ionization, nanoelectrospray, andLC/MS sample pretreatment in proteomics. Anal Chem 75:663-670
22. Cox J et al (2009) A practical guide to the MaxQuant computational platform for SILACbased quantitative proteomics. Nat Protoc 4:698-705
23. Zanivan S, Krueger M, Mann M (2012) In vivo quantitative proteomics: the SILAC mouse. Methods Mol Biol 757:435-450
24. Bicho CC et al (2010) A genetic engineering solution to the "arginine conversion problem" in stable isotope labeling by amino acids in cell culture(SILAC). Mol Cell Proteomics 9:1567-1577