Switchable reporter enzymes based on mutually exclusive domain interactions allow antibody detection directly in solution

ACS Chemical Biology

Volumn 8, Issue 10, 2013, Pages 2127-2132

  Banala, Sambashiva ^a   Aper, Stijn J A ^a   Schalk, Werner ^a   Merkx, Maarten ^a  

^a EINDHOVEN UNIVERSITY OF TECHNOLOGY   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

BETA LACTAMASE; BETA LACTAMASE INHIBITOR; VIRUS ANTIBODY; ANTIBODY; SOLUTION AND SOLUBILITY;

ANTIBODY DETECTION; ANTIGEN BINDING; ARTICLE; COLORIMETRY; ENZYME ANALYSIS; ENZYME INHIBITOR INTERACTION; HUMAN; MOLECULAR INTERACTION; NONHUMAN; PRIORITY JOURNAL; PROTEIN DOMAIN; SERODIAGNOSIS; CHEMISTRY; GENETICS; IMMUNOASSAY; LIMIT OF DETECTION; METABOLISM; PROCEDURES; PROTEIN TERTIARY STRUCTURE; SOLUTION AND SOLUBILITY;

DENGUE VIRUS TYPE 1;

ANTIBODIES; BETA-LACTAMASE INHIBITORS; BETA-LACTAMASES; COLORIMETRY; HUMANS; IMMUNOASSAY; LIMIT OF DETECTION; PROTEIN STRUCTURE, TERTIARY; SOLUTIONS;

EID: 84886496265     PISSN: 15548929     EISSN: 15548937     Source Type: Journal    
DOI: 10.1021/cb400406x     Document Type: Article

References (20)

1. Manz, R. A., Hauser, A. E., Hiepe, F., and Radbruch, A. (2005) Maintenance of serum antibody levels Annu. Rev. Immunol. 23, 367-386
2. Gubala, V., Harris, L. F., Ricco, A. J., Tan, M. X., and Williams, D. E. (2012) Point of care diagnostics: Status and future Anal. Chem. 84, 487-515
3. Golynskiy, M. V., Koay, M. S., Vinkenborg, J. L., and Merkx, M. (2011) Engineering protein switches: Sensors, regulators, and spare parts for biology and biotechnology ChemBioChem 12, 353-361
4. Ostermeier, M. (2009) Designing switchable enzymes Curr. Opin. Struct. Biol. 19, 442-448
5. Ferrer-Miralles, N., Feliu, J. X., Vandevuer, S., Müller, A., Cabrera-Crespo, J., Ortmans, I., Hoffmann, F., Cazorla, D., Rinas, U., Prévost, M., and Villaverde, A. (2001) Engineering regulable Escherichia coli β-galactosidases as biosensors for anti-HIV antibody detection in human sera J. Biol. Chem. 276, 40087-40095
6. Legendre, D., Soumillion, P., and Fastrez, J. (1999) Engineering a regulatable enzyme for homogeneous immunoassays Nat. Biotechnol. 17, 67-72
7. Brennan, C., Christianson, K., Surowy, T., and Mandecki, W. (1994) Modulation of enzyme activity by antibody binding to an alkaline phosphatase-epitope hybrid protein Protein Eng. 7, 509-514
8. Brennan, C. A., Christianson, K., La Fleur, M. A., and Mandecki, W. (1995) A molecular sensor system based on genetically engineered alkaline phosphatase Proc. Natl. Acad. Sci. U.S.A. 92, 5783-5787
9. Fastrez, J. (2009) Engineering allosteric regulation into biological catalysts ChemBioChem 10, 2824-2835
10. Geddie, M. L. and Matsumura, I. (2007) Antibody-induced oligomerization and activation of an engineered reporter enzyme J. Mol. Biol. 369, 1052-1059
11. de las Heras, R., Fry, S. R., Li, J., Arel, E., Kachab, E. H., Hazell, S. L., and Huang, C.-Y. (2008) Development of homogeneous immunoassays based on protein fragment complementation Biochem. Biophys. Res. Commun. 370, 164-168
12. Golynskiy, M. V., Rurup, W. F., and Merkx, M. (2010) Antibody detection by using a FRET-based protein conformational switch ChemBioChem 11, 2264-2267
13. Oh, K. J., Cash, K. J., and Plaxco, K. W. (2006) Excimer-based peptide beacons: A convenient experimental approach for monitoring polypeptide-protein and polypeptide-oligonucleotide interactions J. Am. Chem. Soc. 128, 14018-14019
14. Huang, W., Beharry, Z., Zhang, Z., and Palzkill, T. (2003) A broad-spectrum peptide inhibitor of β-lactamase identified using phage display and peptide arrays Protein Eng. 16, 853-860
15. Zhang, Z. and Palzkill, T. (2003) Determinants of binding affinity and specificity for the interaction of TEM-1 and SME-1 β-lactamase with β-lactamase inhibitory protein J. Biol. Chem. 278, 45706-45712
16. Strynadka, N. C., Jensen, S. E., Johns, K., Blanchard, H., Page, M., Matagne, A., Frère, J. M., and James, M. N. G. (1994) Structural and kinetic characterization of a β-lactamase-inhibitor protein Nature 368, 657-660
17. Wang, J., Zhang, Z., Palzkill, T., and Chow, D. C. (2007) Thermodynamic investigation of the role of contact residues of β-lactamase-inhibitory protein for binding to TEM-1 β-lactamase J. Biol. Chem. 282, 17676-17684
18. Hanes, M. S., Reynolds, K. A., McNamara, C., Ghosh, P., Bonomo, R. A., Kirsch, J. F., and Handel, T. M. (2011) Specificity and cooperativity at β-lactamase position 104 in TEM-1/BLIP and SHV-1/BLIP interactions Proteins 79, 1267-1276
19. Wu, H. C., Huang, Y.-L., Chao, T.-T., Jan, J.-T., Huang, J.-L., Chiang, H.-Y., King, C.-C., and Shaio, M. F. (2001) Identification of B-cell epitope of dengue virus type 1 and its application in diagnosis of patients J. Clin. Microbiol. 39, 977-982
20. Quan, J. and Tian, J. (2009) Circular polymerase extension cloning of complex gene libraries and pathways PLoS One 4, e6441