Quantitative assessment of Pdx1 promoter activity in vivo using a secreted luciferase reporter system

Endocrinology

Volumn 154, Issue 11, 2013, Pages 4388-4395

  Nishimura, Wataru ^a   Eto, Koki ^a,b   Miki, Atsushi ^c   Goto, Motohito ^a   Kawaguchi, Miho ^a   Nammo, Takao ^a   Udagawa, Haruhide ^a   Hiramoto, Masaki ^a   Shimizu, Yukiko ^a   Okamura, Tadashi ^a   Fujiwara, Toshiyoshi ^b   Yasuda, Yoshikazu ^c   Yasuda, Kazuki ^a  

^a NATIONAL CENTER FOR GLOBAL HEALTH AND MEDICINE   (Japan)

^b OKAYAMA UNIVERSITY   (Japan)

^c JICHI MEDICAL UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

GAUSSIA PRINCEPS LUCIFERASE; LUCIFERASE; TRANSCRIPTION FACTOR PDX 1; UNCLASSIFIED DRUG;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; BIOLUMINESCENCE; CONTROLLED STUDY; DUODENUM; ENZYME ACTIVITY; IMMUNE DEFICIENCY; IMMUNOHISTOCHEMISTRY; MOUSE; NONHUMAN; PANCREAS ISLET BETA CELL; PANCREAS RESECTION; PRIORITY JOURNAL; PROTEIN EXPRESSION; TRANSGENE;

ANIMALS; CELL LINE; COPEPODA; GENE EXPRESSION; GENES, REPORTER; HOMEODOMAIN PROTEINS; ISLETS OF LANGERHANS; LUCIFERASES; MICE; PROMOTER REGIONS, GENETIC; RATS; TRANS-ACTIVATORS;

EID: 84886494348     PISSN: 00137227     EISSN: 19457170     Source Type: Journal    
DOI: 10.1210/en.2012-2248     Document Type: Article

References (30)

1. Yong J, Rasooly J, Dang H, et al. Multimodality imaging of β-cells in mouse models of type 1 and 2 diabetes. Diabetes. 2011;60: 1383-1392.
2. Wurdinger T, Badr C, Pike L, et al. A secreted luciferase for ex vivo monitoring of in vivo processes. Nat Methods. 2008;2: 171-173.
3. Santos EB, Yeh R, Nikhamin Y, et al. Sensitive in vivo imaging of T cells using a membrane-bound Gaussia princeps luciferase. Nat Med. 2009;3: 338-344.
4. Tannous BA. Gaussia luciferase reporter assay for monitoring biological processes in culture and in vivo. Nat Protoc. 2009;4: 582-591.
5. Ruecker O, Zillner K, Groebner-Ferreira R, Heitzer M. Gaussialuciferase as a sensitive reporter gene for monitoring promoter activity in the nucleus of the green alga Chlamydomonas reinhardtii. Mol Genet Genomics. 2008;280: 153-162.
6. Oliver-Krasinski JM, Stoffers DA. On the origin of the beta cell. Genes Dev. 2008;22: 1998-2021.
7. Okamura T, Miyoshi I, Takahashi K, Mototani Y, Ishigaki S, Kon Y, Kasai N. Bilateral congenital cataracts result from a gain-of-function mutation in the gene for aquaporin-0 in mice. Genomics. 2003; 81: 361-368.
8. Nishimura W, Rowan S, Salameh T, et al. Preferential reduction of β cells derived from Pax6-MafB pathway in MafB deficient mice. Dev Biol. 2008;314: 443-456.
9. Tannous BA, Kim DE, Fernandez JL, Weissleder R, Breakefield XO. Codon-optimized Gaussia luciferase cDNA for mammalian gene expression in culture and in vivo. Mol Ther. 2005;3: 435-443.
10. Gannon M, Gamer LW, Wright CV. Regulatory regions driving developmental and tissue-specific expression of the essential pancreatic gene pdx1. Dev Biol. 2001;238: 185-201.
11. Fujitani Y, Fujitani S, Boyer DF, et al. Targeted deletion of a cisregulatory region reveals differential gene dosage requirements for Pdx1 in foregut organ differentiation and pancreas formation. Genes Dev. 2006;20: 253-266.
12. Park SY,WangX,ChenZ, et al. Optical imaging of pancreatic β cells in living mice expressing a mouse insulin I promoter-firefly luciferase transgene. Genesis. 2005;43: 80-86.
13. Virostko J, Radhika A, Poffenberger G, et al. Bioluminescence imaging in mouse models quantifies beta cell mass in the pancreas and after islet transplantation. Mol Imaging Biol. 2010;12: 42-53.
14. Bonner-Weir S, Trent DF, Weir GC. Partial pancreatectomy in the rat and subsequent defect in glucose-induced insulin release. J Clin Invest. 1983;71: 1544-1553.
15. Ku GM, Kim H, Vaughn IW, et al. Research resource: RNA-Seq reveals unique features of the pancreatic β-cell transcriptome. Mol Endocrinol. 2012;10: 1783-1792.
16. Shiraiwa T, Kaneto H, Miyatsuka T, et al. Establishment of a noninvasive mouse reporter model for monitoring in vivo pdx-1 promoter activity. Biochem Biophys Res Commun. 2007;361: 739-744.
17. Liu A, Desai BM, Stoffers DA. Identification of PCIF1, a POZ domain protein that inhibits PDX-1 (MODY4) transcriptional activity. Mol Cell Biol. 2004;24: 4372-4383.
18. Mukai E, Toyoda K, Kimura H, et al. GLP-1 receptor antagonist as a potential probe for pancreatic β-cell imaging. Biochem Biophys Res Commun. 2009;389: 523-526.
19. Smith SJ, Zhang H, Clermont AO, et al. In vivo monitoring of pancreatic β-cells in a transgenic mouse model. Mol Imaging. 2006;5: 65-75.
20. Souza F, Simpson N, Raffo A, et al. Longitudinal noninvasive PETbased β cell mass estimates in a spontaneous diabetes rat model. J Clin Invest. 2006;116: 1506-1513.
21. Goland R, Freeby M, Parsey R, et al. 11C-dihydrotetrabenazine PET of the pancreas in subjects with long-standing type 1 diabetes and in healthy controls. J Nucl Med. 2009;50: 382-389.
22. Reiner T, Thurber G, Gaglia J, et al. Accurate measurement of pancreatic islet β-cell mass using a second-generation fluorescent exendin- 4 analog. Proc Natl Acad Sci USA. 2011;108: 12815-12820.
23. Lu Y, Dang H, Middleton B, et al. Bioluminescent monitoring of islet graft survival after transplantation. Mol Ther. 2004;9: 428-435.
24. Fowler M, Virostko J, Chen Z, et al. Assessment of pancreatic islet mass after islet transplantation using in vivo bioluminescence imaging. Transplantation. 2005;79: 768-776.
25. Kim SJ, Doudet DJ, Studenov AR, et al. Quantitative micro positron emission tomography (PET) imaging for the in vivo determination of pancreatic islet graft survival. Nat Med. 2006;12: 1423-1428.
26. McGirr R, Hu S, Yee SP, Kovacs MS, Lee TY, Dhanvantari S. Towards PET imaging of intact pancreatic β cell mass: a transgenic strategy. Mol Imaging Biol. 2011;13: 962-972.
27. Sever D, Eldor R, Sadoun G, et al. Evaluation of impaired β-cell function in nonobese-diabetic (NOD) mouse model using bioluminescence imaging. FASEB J. 2011;2: 676-684.
28. Campbell SC, Macfarlane WM. Regulation of the pdx1 gene promoter in pancreatic β-cells. Biochem Biophys Res Commun. 2002; 299: 277-284.
29. Zhao H, Doyle TC, Wong RJ, et al. Characterization of coelenterazine analogs for measurements of renilla luciferase activity in live cells and living animals. Mol Imaging. 2004;3: 43-54.
30. Hawkins E, Unch J, Murphy N, et al. Measuring Renilla luciferase luminescence in living cells. Promega Notes. 2005;90: 10-14.