Gaussia-luciferase as a sensitive reporter gene for monitoring promoter activity in the nucleus of the green alga Chlamydomonas reinhardtii

Molecular Genetics and Genomics

Volumn 280, Issue 2, 2008, Pages 153-162

  Ruecker, Ovidiu ^a   Zillner, Karina ^a   Groebner Ferreira, Regina ^a   Heitzer, Markus ^a,b  

^a UNIVERSITY OF REGENSBURG   (Germany)

^b Geneart AG   (Germany)

Author keywords

Cauliflower mosaic virus; Chlamydomonas reinhardtii; Gaussia princeps; Luciferase; Promoter

Indexed keywords

LUCIFERASE; PROTEIN; SIGNAL PEPTIDE;

5' UNTRANSLATED REGION; ARTICLE; CAULIFLOWER MOSAIC VIRUS; CHLAMYDOMONAS REINHARDTII; CONTROLLED STUDY; CULTURE MEDIUM; GAUSSIA; GENE EXPRESSION; GENETIC ANALYSIS; GENOME; GREEN ALGA; LUMINESCENCE; MONITORING; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN EXPRESSION; PROTEIN SECRETION; RENILLA; REPORTER GENE; TEMPERATURE; TRANSGENE; VIRUS GENE; WILD TYPE;

AMINO ACID SEQUENCE; ANIMALS; BASE SEQUENCE; CAULIMOVIRUS; CELL NUCLEUS; CHLAMYDOMONAS REINHARDTII; COPEPODA; DNA, ALGAL; DNA, PROTOZOAN; GENE EXPRESSION; GENES, PROTOZOAN; GENES, REPORTER; GENES, VIRAL; LUCIFERASES; MOLECULAR SEQUENCE DATA; PROMOTER REGIONS (GENETICS);

ALGAE; CAULIFLOWER MOSAIC VIRUS; CHLAMYDOMONAS; CHLAMYDOMONAS REINHARDTII; CHLOROPHYTA; GAUSSIA PRINCEPS (T. SCOTT, 1894); RENILLA; RENILLA RENIFORMIS;

EID: 48349095382     PISSN: 16174615     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00438-008-0352-3     Document Type: Article

References (44)

1. K Babinger A Hallmann R Schmitt 2006 Translational control of regA, a key gene controlling cell differentiation in Volvox carteri Development 133 4045 4051
2. MJ Battraw TC Hall 1990 Histochemical analysis of CaMV 35S promoter-beta-glucuronidase gene expression in transgenic rice plants Plant Mol Biol 15 527 538
3. JE Blankenship KL Kindle 1992 Expression of chimeric genes by the light-regulated cabII-1 promoter in Chlamydomonas reinhardtii: a cabII-1/nit1 gene functions as a dominant selectable marker in a nit1- nit2- strain Mol Cell Biol 12 5268 5279
4. MT Croft M Moulin ME Webb AG Smith 2007 Thiamine biosynthesis in algae is regulated by riboswitches Proc Natl Acad Sci USA 104 20770 20775
5. JP Davies DP Weeks AR Grossman 1992 Expression of the arylsulfatase gene from the beta 2-tubulin promoter in Chlamydomonas reinhardtii Nucleic Acids Res 20 2959 2965
6. A Day R Debuchy J van Dillewijn S Purton JD Rochaix 1990 Studies on the maintenance and expression of cloned DNA fragments in the nuclear genome of the green alga Chlamydomonas reinhardtii Physiol Plant 78 254 260
7. EL de Hostos RK Togasaki A Grossman 1988 Purification and biosynthesis of a derepressible periplasmic arylsulfatase from Chlamydomonas reinhardtii J Cell Biol 106 29 37
8. M Fuhrmann 2002 Expanding the molecular toolkit for Chlamydomonas reinhardtii-from history to new frontiers Protist 153 357 364
9. M Fuhrmann A Hausherr L Ferbitz T Schodl M Heitzer P Hegemann 2004 Monitoring dynamic expression of nuclear genes in Chlamydomonas reinhardtii by using a synthetic luciferase reporter gene Plant Mol Biol 55 869 881
10. M Fuhrmann W Oertel P Hegemann 1999 A synthetic gene coding for the green fluorescent protein (GFP) is a versatile reporter in Chlamydomonas reinhardtii Plant J 19 353 361
11. CM Griffiths MJ Page 1997 Production of heterologous proteins using the baculovirus/insect expression system Methods Mol Biol 75 427 440
12. Harris EH (1989) The Chlamydomonas sourcebook: a comprehensive guide to biology and laboratory use. Academic Press, San Diego
13. EH Harris 2001 Chlamydomonas as a model organism Annu Rev Plant Physiol Plant Mol Biol 52 363 406
14. SE Hasnain EK Manavathu WC Leung 1985 DNA-mediated transformation of Chlamydomonas reinhardi cells: use of aminoglycoside 3′-phosphotransferase as a selectable marker Mol Cell Biol 5 3647 3650
15. Heitzer M, Zschoernig B (2007) Construction of modular tandem expression vectors for the green alga Chlamydomonas reinhardtii using the Cre/lox-system. Biotechniques 43:324, 326, 328 passim
16. S Inouye Y Sahara 2008 Identification of two catalytic domains in a luciferase secreted by the copepod Gaussia princeps Biochem Biophys Res Commun 365 96 101
17. K Julenius A Molgaard R Gupta S Brunak 2005 Prediction, conservation analysis, and structural characterization of mammalian mucin-type O-glycosylation sites Glycobiology 15 153 164
18. M Kang DD Duncan JL van Etten 2005 Chlorovirus: a genus of Phycodnaviridae that infects certain chlorella-like green algae Mol Plant Pathol 6 213 224
19. M Kang JG Han PF Liu Y Ye P Tien 2000 [The regulation activity of Chlorella virus gene 5′ upstream sequence in Escherichia coli and eucaryotic alage] Sheng Wu Gong Cheng Xue Bao 16 443 446
20. S Kiaulehn O Voytsekh M Fuhrmann M Mittag 2007 The presence of UG-repeat sequences in the 3′-UTRs of reporter luciferase mRNAs mediates circadian expression and can determine acrophase in Chlamydomonas reinhardtii J Biol Rhythms 22 275 277
21. KL Kindle RA Schnell E Fernandez PA Lefebvre 1989 Stable nuclear transformation of Chlamydomonas using the Chlamydomonas gene for nitrate reductase J Cell Biol 109 2589 2601
22. K Kobayashi S Tsuge L Stavolone T Hohn 2002 The Cauliflower mosaic virus virion-associated protein is dispensable for viral replication in single cells J Virol 76 9457 9464
23. GF Kutish Y Li Z Lu M Furuta DL Rock JL Van Etten 1996 Analysis of 76 kb of the chlorella virus PBCV-1 330-kb genome: map positions 182 to 258 Virology 223 303 317
24. T Matsuo K Onai K Okamoto J Minagawa M Ishiura 2006 Real-time monitoring of chloroplast gene expression by a luciferase reporter: evidence for nuclear regulation of chloroplast circadian period Mol Cell Biol 26 863 870
25. SP Mayfield KL Kindle 1990 Stable nuclear transformation of Chlamydomonas reinhardtii by using a C. reinhardtii gene as the selectable marker Proc Natl Acad Sci USA 87 2087 2091
26. SP Mayfield J Schultz 2004 Development of a luciferase reporter gene, luxCt, for Chlamydomonas reinhardtii chloroplast Plant J 37 449 458
27. I Minko SP Holloway S Nikaido M Carter OW Odom CH Johnson DL Herrin 1999 Renilla luciferase as a vital reporter for chloroplast gene expression in Chlamydomonas Mol Gen Genet 262 421 425
28. KE Narva DL Wendell MP Skrdla JL Van Etten 1987 Molecular cloning and characterization of the gene encoding the DNA methyltransferase, M.CviBIII, from Chlorella virus NC-1A Nucleic Acids Res 15 9807 9823
29. DW Ow JD Jacobs SH Howell 1987 Functional regions of the Cauliflower mosaic virus 35S RNA promoter determined by use of the firefly luciferase gene as a reporter of promoter activity Proc Natl Acad Sci USA 84 4870 4874
30. MM Pooggin J Futterer KG Skryabin T Hohn 2001 Ribosome shunt is essential for infectivity of Cauliflower mosaic virus Proc Natl Acad Sci USA 98 886 891
31. MV Rajam SV Kumar 2006 Green Alga (Chlamydomonas reinhardtii) Methods Mol Biol 344 421 433
32. JD Rochaix 1995 Chlamydomonas reinhardtii as the photosynthetic yeast Annu Rev Genet 29 209 230
33. LA Ryabova T Hohn 2000 Ribosome shunting in the Cauliflower mosaic virus 35S RNA leader is a special case of reinitiation of translation functioning in plant and animal systems Genes Dev 14 817 829
34. M Schroda 2006 RNA silencing in Chlamydomonas: mechanisms and tools Curr Genet 49 69 84
35. N Shao A Krieger-Liszkay M Schroda CF Beck 2007 A reporter system for the individual detection of hydrogen peroxide and singlet oxygen: its use for the assay of reactive oxygen species produced in vivo Plant J 50 475 487
36. PJ Southern P Berg 1982 Transformation of mammalian cells to antibiotic resistance with a bacterial gene under control of the SV40 early region promoter J Mol Appl Genet 1 327 341
37. DR Stevens JD Rochaix S Purton 1996 The bacterial phleomycin resistance gene ble as a dominant selectable marker in Chlamydomonas Mol Gen Genet 251 23 30
38. S Tabor CC Richardson 1985 A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genes Proc Natl Acad Sci USA 82 1074 1078
39. DK Tang SY Qiao M Wu 1995 Insertion mutagenesis of Chlamydomonas reinhardtii by electroporation and heterologous DNA Biochem Mol Biol Int 36 1025 1035
40. BA Tannous DE Kim JL Fernandez R Weissleder XO Breakefield 2005 Codon-optimized Gaussia luciferase cDNA for mammalian gene expression in culture and in vivo Mol Ther 11 435 443
41. O Vallon FA Wollman 1995 Mutations affecting O-glycosylation in Chlamydomonas reinhardtii cause delayed cell wall degradation and sex-limited sterility Plant Physiol 108 703 712
42. M Verhaegent TK Christopoulos 2002 Recombinant Gaussia luciferase. Overexpression, purification, and analytical application of a bioluminescent reporter for DNA hybridization Anal Chem 74 4378 4385
43. S Wiles K Ferguson M Stefanidou DB Young BD Robertson 2005 Alternative luciferase for monitoring bacterial cells under adverse conditions Appl Environ Microbiol 71 3427 3432
44. F Wurm A Bernard 1999 Large-scale transient expression in mammalian cells for recombinant protein production Curr Opin Biotechnol 10 156 159