Yeast two-hybrid analysis of jasmonate signaling proteins

Methods in Molecular Biology

Volumn 1011, Issue , 2013, Pages 173-185

  Cuéllar, Amparo Pérez ^a,b   Pauwels, Laurens ^a,b   De Clercq, Rebecca ^a,b   Goossens, Alain ^a,b  

^a DEPARTMENT OF PLANT SYSTEMS BIOLOGY   (Belgium)

^b GHENT UNIVERSITY   (Belgium)

Author keywords

Jasmonate signaling; Protein complexes; Protein domain; Protein protein interaction; Yeast three hybrid; Yeast two hybrid

Indexed keywords

JASMONIC ACID; ARABIDOPSIS PROTEIN; SIGNAL PEPTIDE;

ARTICLE; CONTROLLED STUDY; FUNGAL GENE; GENE EXPRESSION; NONHUMAN; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN PROTEIN INTERACTION; REPORTER GENE; SIGNAL TRANSDUCTION; TWO HYBRID SYSTEM; YEAST; ARABIDOPSIS; BIOSYNTHESIS; CHEMISTRY; ESCHERICHIA COLI; GENE VECTOR; GENETIC TRANSFORMATION; GENETICS; PLASMID; PROTEIN BINDING; SACCHAROMYCES CEREVISIAE;

ARABIDOPSIS; ARABIDOPSIS PROTEINS; ESCHERICHIA COLI; GENETIC VECTORS; INTRACELLULAR SIGNALING PEPTIDES AND PROTEINS; PLASMIDS; PROTEIN BINDING; SACCHAROMYCES CEREVISIAE; TRANSFORMATION, GENETIC; TWO-HYBRID SYSTEM TECHNIQUES;

EID: 84886400349     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-414-2_14     Document Type: Article

References (23)

1. Pauwels L, Goossens A (2011) The JAZ proteins: a crucial interface in the jasmonate signaling cascade. Plant Cell 23:3089-3100
2. Brückner A, Polge C, Lentze N, Auerbach D, Schlattner U (2009) Yeast two-hybrid, a powerful tool for systems biology. Int J Mol Sci 10:2763-2788
3. Shoemaker BA, Panchenko AR (2007) Deciphering protein-protein interactions. Part I. experimental techniques and databases. PLoS Comput Biol 3:e42
4. Berggård T, Linse S, James P (2007) Methods for the detection and analysis of protein-protein interactions. Proteomics 7:2833-2842
5. Thines B, Katsir L, Melotto M, Niu Y, Mandaokar A, Liu G, Nomura K, He SY, Howe GA, Browse J (2007) JAZ repressor proteins are targets of the SCF COI1 complex during jasmonate signalling. Nature 448:661-665
6. Causier B (2004) Studying the interactome with the yeast two-hybrid system and mass spectrometry. Mass Spectrom Rev 23:350-367
7. Cusick ME, Klitgord N, Vidal M, Hill DE (2005) Interactome: gateway into systems biology. Hum Mol Genet 14:R171-R181
8. Pauwels L, Fernández Barbero G, Geerinck J, Tilleman S, Grunewald W, Cuéllar Pérez A, Chico JM, Vanden Bossche R, Sewell J, Gil E, García-Casado G, Witters E, Inzé D, Long JA, De Jaeger G, Solano R, Goossens A (2010) NINJA connects the co-repressor TOPLESS to jasmonate signalling. Nature 464:788-791
9. Fields S, O-k S (1989) A novel genetic system to detect protein-protein interactions. Nature 340:245-246
10. Causier B, Davies B (2002) Analysing proteinprotein interactions with the yeast two-hybrid system. Plant Mol Biol 50:855-870
11. Causier B, Ashworth M, Guo W, Davies B (2012) The TOPLESS interactome: a framework for gene repression in Arabidopsis. Plant Physiol 158:423-438
12. Arabidopsis Interactome Mapping Consortium (2011) Evidence for network evolution in an Arabidopsis interactome map. Science 333:601-607
13. Nagels Durand A, Moses T, De Clercq R, Goossens A, Pauwels L (2012) A MultiSite Gateway™ vector set for the functional analysis of genes in the model Saccharomyces cerevisiae . BMC Mol Biol 13:30
14. Estojak J, Brent R, Golemis EA (1995) Correlation of two-hybrid af finity data with in vitro measurements. Mol Cell Biol 15:5820-5829
15. Gyuris J, Golemis E, Chertkov H, Brent R (1993) Cdi1, a human G1 and S phase protein phosphatase that associates with Cdk2. Cell 75:791-803
16. Gietz RD, Schiestl RH (2007) High-ef ficiency yeast transformation using the LiAc/SS carrier DNA/PEG method. Nat Protoc 2:31-34
17. Bergman L (2001) Growth and maintenance of yeast. Methods Mol Biol 177:9-14
18. Saghbini M, Hoekstra D, Gautsch J (2001) Media formulations for various two-hybrid systems. Methods Mol Biol 177:15-39
19. Chini A, Fonseca S, Chico JM, Fernández-Calvo P, Solano R (2009) The ZIM domain mediates homo-and heteromeric interactions between Arabidopsis JAZ proteins. Plant J 59:77-87
20. Chung HS, Howe GA (2009) A critical role for the TIFY motif in repression of jasmonate signaling by a stabilized splice variant of the JASMONATE ZIM-domain protein JAZ10 in Arabidopsis . Plant Cell 21:131-145
21. Rajagopala SV, Hughes KT, Uetz P (2009) Benchmarking yeast two-hybrid systems using the interactions of bacterial motility proteins. Proteomics 9:5296-5302
22. James P, Halladay J, Craig EA (1996) Genomic libraries and a host strain designed for highly ef ficient two-hybrid selection in yeast. Genetics 144:1425-1436
23. Gietz RD, Schiestl RH (2007) Frozen competent yeast cells that can be transformed with high ef ficiency using the LiAc/SS carrier DNA/PEG method. Nat Protoc 2:1-4