Functional expression of hepassocin in Escherichia coli using SUMO fusion partner and molecular chaperones

Protein Expression and Purification

Volumn 92, Issue 2, 2013, Pages 135-140

  Wang, Qi ^a   Zhao, Jiaojiao ^a   Wang, Yan ^a   Sun, Honglou ^a   Jiang, Yi ^a   Luo, Lan ^b   Yin, Zhimin ^a  

^a NANJING NORMAL UNIVERSITY   (China)

^b NANJING UNIVERSITY   (China)

Author keywords

Co expression; Escherichia coli; Human hepassocin; Molecular chaperone; Small ubiquitin related modifier; Soluble expression

Indexed keywords

CHAPERONE; FGL1 PROTEIN, HUMAN; HYBRID PROTEIN; PEPTIDE HYDROLASE; SUMO PROTEIN; TUMOR PROTEIN;

AFFINITY CHROMATOGRAPHY; ARTICLE; CELL LINE; CELL PROLIFERATION; CHEMISTRY; CO-EXPRESSION; DRUG EFFECT; ESCHERICHIA COLI; FERMENTATION; GENETICS; HUMAN; HUMAN HEPASSOCIN; METABOLISM; MOLECULAR CLONING; SMALL UBIQUITIN-RELATED MODIFIER; SOLUBLE EXPRESSION;

CO-EXPRESSION; ESCHERICHIA COLI; HUMAN HEPASSOCIN; MOLECULAR CHAPERONE; SMALL UBIQUITIN-RELATED MODIFIER; SOLUBLE EXPRESSION;

CELL LINE; CELL PROLIFERATION; CHROMATOGRAPHY, AFFINITY; CLONING, MOLECULAR; ESCHERICHIA COLI; FERMENTATION; HUMANS; MOLECULAR CHAPERONES; NEOPLASM PROTEINS; PEPTIDE HYDROLASES; RECOMBINANT FUSION PROTEINS; SMALL UBIQUITIN-RELATED MODIFIER PROTEINS;

EID: 84886313395     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2013.09.014     Document Type: Article

References (34)

1. W.E. Fleig Liver-specific growth factors Scand. J. Gastroenterol. Suppl. 151 1988 31 36 (Pubitemid 19098623)
2. H. Hara, S. Uchida, H. Yoshimura, M. Aoki, Y. Toyoda, Y. Sakai, S. Morimoto, H. Fukamachi, K. Shiokawa, and K. Hanada Isolation and characterization of a novel liver-specific gene, hepassocin, upregulated during liver regeneration Biochim. Biophys. Acta 1492 2000 31 44 (Pubitemid 30349178)
3. J. Nilsson, S. Stahl, J. Lundeberg, M. Uhlen, and P.A. Nygren Affinity fusion strategies for detection, purification, and immobilization of recombinant proteins Protein Expr. Purif. 119 1997 1 16 (Pubitemid 27434126)
4. P. Jonasson, S. Liljeqvist, P.A. Nygren, and S. Stahl Genetic design for facilitated production and recovery of recombinant proteins in Escherichia coli Appl. Biochem. Biotechnol. 35 2002 91 105 (Pubitemid 34279403)
5. X. Zuo, M.R. Mattern, R. Tan, S. Li, J. Hall, D.E. Sterner, J. Shoo, H. Tran, P. Lim, S.G. Sarafianos, L. Kazi, M.S. Navas, S.R. Weiss, and T.R. Butt Expression and purification of SARS coronavirus proteins using SUMO-fusions Protein Expr. Purif. 42 2005 100 110 (Pubitemid 40793399)
6. W.X. Xu, M.M. Cao, Z.D. Wang, C.H. Ge, M. Yu, H.T. Yu, Y.Q. Zhan, and X.M. Yang Expression and purification of soluble human hepassocin in Escherichia coli Lett. Biotechnol. 20 2009 491 494 [In Chinese]
7. Z. Sun, Z. Xia, F. Bi, and J.N. Liu Expression and purification of human urodilatin by small ubiquitin-related modifier fusion in Escherichia coli Appl. Microbiol. Biotechnol. 78 2008 495 502
8. C.Y. Li, C.Z. Cao, W.X. Xu, M.M. Cao, F. Yang, L. Dong, M. Yu, Y.Q. Zhan, Y.B. Gao, W. Li, Z.D. Wang, C.H. Ge, Q.M. Wang, R.Y. Peng, and X.M. Yang Recombinant human hepassocin stimulates proliferation of hepatocytes in vivo and improves survival in rats with fulminant hepatic failure Gut 59 2010 817 826
9. S. Jana, and J.K. Deb Strategies for efficient production of heterologous proteins in Escherichia coli Appl. Microbiol. Biotechnol. 67 2005 289 298 (Pubitemid 40684730)
10. K. Nishihara, M. Kanemori, M. Kitagawa, H. Yanagi, and T. Yura Chaperone co-expression plasmids: differential and synergistic roles of DnaK-DnaJ-GrpE and GroEL-GroES in assisting folding of an allergen of Japanese cedar pollen, Cryj2, in Escherichia coli Appl. Environ. Microbiol. 64 1998 1694 1699 (Pubitemid 28234771)
11. R. Novy, D. Drott, K. Yaeger, and R. Mierendorf Overcoming the codon bias of E. coli for enhanced protein expression Innovations 12 2001 1 3
12. A. Marco, E. Deuerling, A. Mogk, T. Tomoyasu, and B. Bukau Chaperone-based procedure to increase yields of soluble recombinant proteins produced in E. coli BMC Biotechnol. 7 2007 32
13. U.K. Laemmli Cleavage of structural proteins during the assembly of the head of bacteriophage T4 Nature 277 1970 680 685
14. Y.F. Wu, Y.M. Fan, B. Xue, L. Luo, J.Y. Shen, S.Q. Zhang, Y. Jiang, and Z.M. Yin Human glutathione S-transferase P1-1 interacts with TRAF2 and regulates TRAF2-ASK1 signals Oncogene 25 2006 5787 5800 (Pubitemid 44435255)
15. X.Y. Ma, W.Y. Zheng, D.Z. Wei, Y.S. Ma, T.W. Wang, J.Z. Wang, Q.H. Liu, and S.L. Yang High-level expression, purification and pro-apoptosis activity of HIV-TAT-survivin (T34A) mutant to cancer cells in vitro J. Biotechnol. 123 2006 367 378
16. H. Mirzahoseini, and M. Alibolandi Stability of recombinant proteins in Escherichia coli: the effect of co-expression of five different chaperone sets J. Sci. 20 2009 305 310
17. S.H. Pyo, J.H. Lee, H.B. Park, J.S. Cho, H.R. Kim, B.H. Han, and Y.S. Park Expression and purification of a recombinant buforin derivative from Escherichia coli Process Biochem. 39 2004 1731 1736
18. V.S. Skosyrev, N.V. Rudenko, A.V. Yakhnin, V.E. Zagranichny, L.I. Popova, M.V. Zakharov, A.Y. Gorokhovatsky, and L.M. Vinokurov EGFP as a fusion partner for the expression and organic extraction of small polypeptides Protein Expr. Purif. 27 2003 55 62 (Pubitemid 37096470)
19. X.C. Rao, S. Li, J.C. Hu, X.L. Jin, X.M. Hu, J.J. Huang, Z.J. Chen, J.M. Zhu, and F.Q. Hu A novel carrier molecule for high-level expression of peptide antibiotics in Escherichia coli Protein Expr. Purif. 36 2004 11 18 (Pubitemid 38836759)
20. Z. Sun, J. Chen, H. Yao, L. Liu, J. Wang, J. Zhang, and J.N. Liu Use of Ssp dnaB derived mini-intein as a fusion partner for production of recombinant human brain natriuretic peptide in Escherichia coli Protein Expr. Purif. 43 2005 26 32 (Pubitemid 41097381)
21. Z. Xu, Z. Zhong, L. Huang, L. Peng, F. Wang, and P. Cen High-level production of bioactive human beta-defensin-4 in Escherichia coli by soluble fusion expression Appl. Microbiol. Biotechnol. 72 2006 471 479 (Pubitemid 44273072)
22. A. Mogk, M.P. Mayer, and E. Deuerling Mechanisms of protein folding: molecular chaperones and their application in biotechnology ChemBioChem 3 2002 807 814 (Pubitemid 36002295)
23. E. Deuerling, and B. Bukau Chaperone-assisted folding of newly synthesized proteins in the cytosol Crit. Rev. Biochem. Mol. Biol. 39 2004 261 277 (Pubitemid 40082762)
24. F.U. Hartl, and M. Hayer-Hartl Molecular chaperones in the cytosol: from nascent chain to folded protein Science 295 2002 1852 1858 (Pubitemid 34214115)
25. T. Tomoyasu, A. Mogk, H. Langen, P. Goloubinoff, and B. Bukau Genetic dissection of the roles of chaperones and proteases in protein folding and degradation in the Escherichia coli cytosol Mol. Microbiol. 40 2001 397 413 (Pubitemid 32391634)
26. P.A. Voziyan, B.C. Tieman, C.M. Low, and M.T. Fisher Changing the nature of the initial chaperonin capture complex influences the substrate folding efficiency J. Biol. Chem. 273 1998 25073 25078 (Pubitemid 28443264)
27. F. Baneyx, and M. Mujacic Recombinant protein folding and misfolding in Escherichia coli Nat. Biotechnol. 22 2004 1399 1408
28. E. Deuerling, A. Schulze-Specking, T. Tomoyasu, A. Mogk, and B. Bukau Trigger factor, DnaK cooperate in folding of newly synthesized proteins Nature 400 1999 693 696
29. Q.A. Valent, J.L. Gier, G.V. Heijne, and D.A. Kendall Nascent membrane and presecretory proteins synthesized in Escherichia coli associate with signal recognition particle and trigger factor Mol. Microbiol. 25 1997 53 64 (Pubitemid 27316121)
30. E. Schaffitzel, S. Rüdiger, B. Bukau, and E. Deuerling Functional dissection of trigger factor and DnaK: interactions with nascent polypeptides and thermally denatured proteins Biol. Chem. 382 2001 1235 1243 (Pubitemid 32894030)
31. M.J. Kerner, D.J. Naylor, Y. Ishihama, T. Maier, H.C. Chang, A.P. Stines, C. Georgopoulos, D. Frishman, M. Hayer-Hartl, M. Mann, and F.U. Hartl Proteomewide analysis of chaperonin-dependent protein folding in Escherichia coli Cell 122 2005 209 220 (Pubitemid 41032985)
32. S.D. Weeks, M. Drinker, and P.J. Loll Ligation independent cloning vectors for expression of SUMO fusions Protein Expr. Purif. 53 2007 40 50 (Pubitemid 46283468)
33. J.G. Marblestone, S.C. Edavettal, Y. Lim, P. Lim, X. Zuo, and T.R. Butt Comparison of SUMO fusion technology with traditional gene fusion systems: enhanced expression and solubility with SUMO Protein Sci. 15 2006 182 189 (Pubitemid 43004245)
34. T.R. Butt, S.C. Edavettal, J.P. Hall, and M.R. Mattern SUMO fusion technology for difficult-to-express proteins Protein Expr. Purif. 43 2005 1 9 (Pubitemid 41097378)