N-acetylcysteine protects against fluoride-induced oxidative damage in primary rat hepatocytes

Toxicology in Vitro

Volumn 27, Issue 8, 2013, Pages 2279-2282

  Pawłowska Góral, Katarzyna ^a   Kurzeja, Ewa ^a   Stec, Małgorzata ^a  

^a MEDICAL UNIVERSITY OF SILESIA   (Poland)

Author keywords

Fluoride; Fluorosis; N acetylcysteine; Oxidative stress; Rat hepatocytes

Indexed keywords

ACETYLCYSTEINE; FLUORIDE; GLUTATHIONE; GLUTATHIONE PEROXIDASE; GLUTATHIONE REDUCTASE; LACTATE DEHYDROGENASE; SUPEROXIDE DISMUTASE;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANTIOXIDANT ACTIVITY; ARTICLE; CELL CULTURE; CELL DAMAGE; CELL VIABILITY; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ASSAY; FLUOROSIS; INCUBATION TIME; LIPID PEROXIDATION; LIVER CELL; LIVER PROTECTION; LIVER TOXICITY; NONHUMAN; OXIDATIVE STRESS; PROTEIN FUNCTION; RAT;

FLUORIDE; FLUOROSIS; N-ACETYLCYSTEINE; OXIDATIVE STRESS; RAT HEPATOCYTES;

ACETYLCYSTEINE; ANIMALS; ANTIOXIDANTS; CELLS, CULTURED; GLUTATHIONE; GLUTATHIONE PEROXIDASE; GLUTATHIONE REDUCTASE; HEPATOCYTES; OXIDATIVE STRESS; RATS; RATS, WISTAR; SODIUM FLUORIDE; SUPEROXIDE DISMUTASE;

EID: 84886248481     PISSN: 08872333     EISSN: 18793177     Source Type: Journal    
DOI: 10.1016/j.tiv.2013.09.019     Document Type: Article

References (15)

1. Agalakova N.I., Gusev G.P. Fluoride induces oxidative stress and ATP depletion in the rat erythrocytes in vitro. Environ. Toxicol. Pharmacol. 2012, 34:334-337.
2. Altintaş L., Eşsiz D., Eraslan G., Ince S., Arslanbaş E. Prophylactic effect of N-acetylcysteine against sodium fluoride-induced blood oxidative stress in mice. Food Chem. Toxicol. 2010, 48:2838-2841.
3. Aykin-Burns N., Franklin E.A., Ercal N. Effects of N-acetylcysteine on lead-exposed PC-12 cells. Arch. Environ. Contam. Toxicol. 2005, 49:119-123.
4. Bajt M.L., Knight T.R., Lemasters J.J., Jaeschke H. Acetaminophen-induced oxidant stress and cell injury in cultured mouse hepatocytes: protection by N-acetylcysteine. Toxicol. Sci. 2004, 80:343-349.
5. Beauchamp C., Fridovich I. Superoxide dismutase: Improved assays and an assay applicable to acrylamide gels. Anal. Biochem. 1971, 44:276-287.
6. Carvalho M., Remião F., Milhazes N., Borges F., Fernandes E., Carvalho F., Bastos M.L. The toxicity of N-methyl-alpha-methyldopamine to freshly isolated rat hepatocytes is prevented by ascorbic acid and N-acetylcysteine. Toxicology 2004, 200:193-203.
7. Goldberg M.D., Spooner J.R. Glutathione reductase. Methods. Enzymatic. Anal. 1983, vol. 3:258-265. Academic Press Inc., Florida. third ed.
8. Lévêque V.J., Vance C.K., Nick H.S., Silverman D.N. Redox properties of human manganese superoxide dismutase and active-site mutants. Biochemistry 2001, 40:10586-10591.
9. Lowry O.H., Rosebrough N.J., Farr A.L., Randall R.J. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 1951, 193:265-275.
10. Nabavi S.M., Nabavi S.F., Eslami S., Moghaddam A.H. In vivo protective effects of quercetin against sodium fluoride-induced oxidative stress in the hepatic tissue. Food Chem. 2012, 132:931-935.
11. Paglia D.E., Valentine W.N. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J. Lab. Clin. Med. 1967, 70:158-169.
12. Pawłowska-Góral K., Pilawa B. Detection of free radicals formed by in vitro metabolism of fluoride using EPR spectroscopy. Toxicol. In Vitro. 2011, 25:1269-1273.
13. Philips, D.J., 1978. Dye exclusion test for cell viability. In: Krause, P.F., Paterson, M.H. (Eds.), tissue, culture, methods and application, Acad. Press, New York, pp. 406-408.
14. Stec M., Kurzeja E., Gaweł S., Wardas P., Maciejewska-Paszek I., Pawłowska-Góral K. In vitro studies of the effect of theophylline and/or N-acetylcysteine on redox homeostasis and ketogenesis in rat hepatocytes. Bull. Vet. Inst. Pulawy 2011, 55:519-524.
15. Warren J.J., Levy S.M. Current and future role of fluoride in nutrition. Dent. Clin. North Am. 2003, 47:225-243.