Overexpression, purification and enzymatic characterization of a recombinant plastidial glucose-6-phosphate dehydrogenase from barley (Hordeum vulgare cv. Nure) roots

Plant Physiology and Biochemistry

Volumn 73, Issue , 2013, Pages 266-273

  Cardi, Manuela ^a,b,c   Chibani, Kamel ^b,c   Castiglia, Daniela ^a   Cafasso, Donata ^a   Pizzo, Elio ^a   Rouhier, Nicolas ^b,c   Jacquot, Jean Pierre ^b,c   Esposito, Sergio ^a  

^a UNIVERSITY OF NAPLES FEDERICO II   (Italy)

^b UNIVERSITÉ DE LORRAINE   (France)

^c UNIVERSITÉ DE LORRAINE   (France)

Author keywords

Barley roots; G6PDH; OPPP; Plastidial isoform

Indexed keywords

EMBRYOPHYTA; ESCHERICHIA COLI; HORDEUM; HORDEUM VULGARE; MAMMALIA;

GLUCOSE 6 PHOSPHATE DEHYDROGENASE; ISOPROTEIN; NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; RECOMBINANT PROTEIN; VEGETABLE PROTEIN;

AMINO ACID SEQUENCE; ANIMAL; ARTICLE; BARLEY ROOTS; ENZYMOLOGY; ESCHERICHIA COLI; GENETICS; HORDEUM; MAMMAL; METABOLISM; MOLECULAR GENETICS; OPPP; PLANT GENE; PLANT ROOT; PLASTID; PLASTIDIAL ISOFORM;

BARLEY ROOTS; G6PDH; OPPP; PLASTIDIAL ISOFORM;

AMINO ACID SEQUENCE; ANIMALS; ESCHERICHIA COLI; GENES, PLANT; GLUCOSEPHOSPHATE DEHYDROGENASE; HORDEUM; MAMMALS; MOLECULAR SEQUENCE DATA; NADP; PLANT PROTEINS; PLANT ROOTS; PLASTIDS; PROTEIN ISOFORMS; RECOMBINANT PROTEINS;

EID: 84886237334     PISSN: 09819428     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.plaphy.2013.10.008     Document Type: Article

References (38)

1. Wendt U.K., Hauschild R., Lange C., Pietersma M., Wenderoth I., von Schaewen A. Evidence for functional convergence of redox regulation in G6PDH isoforms of cyanobacteria and higher plants. Plant Mol. Biol. 1999, 40:487-494.
2. Orita I., Sato T., Yurimoto H., Kato N., Atomi H., Imanaka T., Sakai Y. The ribulose monophosphate pathway substitutes for the missing pentose phosphate pathway in the archaeon Thermococcus kodakaraensis. J.Bacteriol. 2006, 188:4698-4704.
3. Dennis D.T., Huang Y., Negm F.B. Glycolysis, the pentose phosphate pathway and anaerobic respiration. Plant Metabolism 1997, 105-127. Longman, London. D.T. Dennis, D.H. Turpin, D.D. Lefebvre, D.B. Layzell (Eds.).
4. Nemoto Y., Sasakuma T. Specific expression of glucose-6-phosphate dehydrogenase (G6PDH) gene by salt stress in wheat (Triticum aestivum L.). Plant Sci. 2000, 158:53-60.
5. Wang X., Ma Y., Huang C., Wan Q., Li N., Bi Y. Glucose-6-phosphate dehydrogenase plays a central role in modulating reduced glutathione levels in reed callus under salt stress. Planta 2008, 227:611-623.
6. Sindelar L., Sindelarova M., Burketova L. Changes in activity of glucose-6-phosphate and 6-phosphogluconate dehydrogenase isozymes upon potato virus Y infection in tobacco leaf tissue and protoplasts. Plant Physiol. Biochem. 1999, 37:195-201.
7. Scharte J., Schön H., Tjaden Z., Weis E. Photosynthesis and carbohydrate metabolism in tobacco leaves during an incompatible interaction with Phytophthora nicotianae. Plant Cell Environ. 2005, 28:1421-1435.
8. Scharte J., Schön H., Tjaden Z., Weis E., von Schaewen A. Isoenzyme replacement of glucose-6-phosphate dehydrogenase in the cytosol improves stress tolerance in plants. Proc. Nat. Acad. Sci. U. S. A. 2009, 106:8061-8066.
9. Wang R., Okamoto M., Xing X., Crawford N.M. Microarray analysis of the nitrate response in Arabidopsis roots and shoots reveals over one thousand rapidly responding genes and new linkages to glucose, trehalose-6-P, iron and sulfate metabolism. Plant Physiol. 2003, 132:556-567.
10. Meyer T., Holscher C., Schwoppe C., von Schaewen A. Alternative targeting of Arabidopsis plastidial glucose-6-phosphate dehydrogenase G6PD1 involves cysteine dependent interaction with G6PD4 in the cytosol. Plant J. 2011, 66:745-758.
11. Cardi M., Chibani K., Cafasso D., Rouhier N., Jacquot J.-P., Esposito S. Abscisic acid effects on activity and expression of different glucose 6 phosphate dehydrogenase isoforms in barley (Hordeum vulgare). J.Exp. Bot. 2011, 62:4013-4023. 10.1093/JXB/ERR100.
12. Kruger N.J., von Schaewen A. The pentose phosphate pathway: structure and organisation. Curr. Op. Plant Biol. 2003, 6:236-246.
13. 13C-labelling: indications for a cytosol and a plastid-localized oxidative pentose phosphate pathway. J.Exp. Bot. 1998, 49:1917-1924.
14. Debnam P.M., Emes M.J. Subcellular distribution of enzymes of the oxidative pentose phosphate pathway in root and leaf tissues. J.Exp. Bot. 1999, 50:1653-1661.
15. von Schaewen A., Langenkamper G., Graeve K., Wenderoth I., Scheibe R. Molecular characterisation of the plastidial glucose-6-phosphate dehydrogenase from potato in comparison to its cytosolic counterpart. Plant Physiol. 1995, 109:1327-1335.
16. Esposito S., Guerriero G., Vona V., Di Martino Rigano V., Carfagna S., Rigano C. Glutamate synthase activities and protein changes in relation to nitrogen nutrition in barley: the dependence on different plastidic glucose-6P dehydrogenase isoforms. J.Exp. Bot. 2005, 56:55-64. 10.1093/jxb/eri006.
17. Knight J.S., Emes M.J., Debnam P.M. Isolation and characterisation of a full-length genomic clone encoding a plastidial glucose 6-phosphate dehydrogenase from Nicotiana tabacum. Planta 2001, 212:499-507.
18. Wakao S., Benning C. Genome-wide analysis of glucose-6-phosphate dehydrogenase in Arabidopsis. Plant J. 2005, 41:243-256.
19. Esposito S., Massaro G., Vona V., Di Martino Rigano V., Carfagna S., Rigano C. Ammonium induction of a novel isoform of glucose-6P dehydrogenase in barley roots. Physiol. Plant. 2001, 113:469-476.
20. Esposito S., Carfagna S., Massaro G., Vona V., Di Martino Rigano V. Glucose-6P dehydrogenase in barley roots: kinetic properties and localisation of the isoforms. Planta 2001, 212:627-634.
21. Schurmann P., Jacquot J.-P. Plant thioredoxin systems revisited. Annu. Rev. Plant Physiol. Plant Mol. Biol. 2000, 51:371-400.
22. Née G., Zaffagnini M., Trost P., Issakidis-Bourguet E. Redox regulation of chloroplastic glucose-6-phosphate dehydrogenase: a new role for f-type thioredoxin. FEBS Lett. 2009, 17:2827-2832.
23. Wendt U.K., Wenderoth I., Tegeler A., von Schaewen A. Molecular characterization of a novel glucose-6-phosphate dehydrogenase from potato (Solanum tuberosum L.). Plant J. 2000, 23:723-733.
24. Bowsher C.G., Hucklesby D.P., Emes M.J. Nitrite reduction and carbohydrate metabolism in plastids purified from roots of Pisum sativum. Planta 1989, 177:359-366.
25. Esposito S., Massaro G., Vona V., Di Martino Rigano V., Carfagna S. Glutamate synthesis in barley roots: the role of the plastidic glucose-6-phosphate dehydrogenase. Planta 2003, 216:639-647.
26. Hutchings D., Rawsthorne S., Emes M.J. Fatty acid synthesis and the oxidative pentose phosphate pathway in developing embryos of oilseed rape (Brassica napus). J.Exp. Bot. 2005, 56:577-585.
27. Honjoh K., Mimura A., Kuroiwa E., Hagisako T., Suga K., Shimizu H., Dubey R.S., Miyamoto T., Hatano S., Iio M. Purification and characterization of two isoforms of glucose 6-phosphate dehydrogenase (G6PDH) from Chlorella vulgaris C-27. Biosci. Biotechnol. Biochem. 2003, 67:1888-1896.
28. Esposito S., Guerriero G., Vona V., Di Martino Rigano V., Carfagna S., Rigano C. Glucose-6P dehydrogenase in Chlorella sorokiniana (211/8k): an enzyme with unusual characteristics. Planta 2006, 223:796-804. 10.1007/s00425-005-0110-2.
29. Ferrara M., Guerriero G., Cardi M., Esposito S. Biochemical characterisation of glucose-6P dehydrogenase from the psychrophilic green alga Koliella antarctica. Extremophiles 2013, 17:53-62. 10.1007/s00792-012-0492-6.
30. Scheibe R., Geissler A., Fickenscher K. Chloroplast glucose-6-phosphate dehydrogenase: Km shift upon light modulation and reduction. Arch. Biochem. Biophys. 1989, 274:290-297.
31. Lee D.W., Kim J.K., Lee S., Choi S., Kim S., Hwang I. Arabidopsis nuclear-encoded plastid transit peptides contain multiple sequence subgroups with distinctive chloroplast-targeting sequence motifs. Plant Cell 2008, 20:1603-1622.
32. Wang X.T., Engel P.C. An optimised system for refolding of human glucose-6-phosphate dehydrogenase. BMC Biotechnol. 2009, 19. 10.1186/1472-6750-9-19.
33. Wang W.T., Au W.N.S., Lam V.M.S., Engel P.C. Recombinant human glucose-6-phosphate dehydrogenase. Evidence for a rapid equilibrium random-order mechanism. Eur. J. Biochem. 2002, 269:3417-3424.
34. Ulusu N.N., Tandogan B., Tezcan F.E. Kinetic properties of glucose-6-phosphate dehydrogenase from lamb kidney cortex. Biochimie 2005, 87:187-190.
35. Dalziel K. Initial steady state velocities in the evaluation of enzyme-coenzyme-substrate reaction mechanisms. Acta Chem. Scand. 1957, 11:1706-1723.
36. Levy H.R. Glucose-6-phosphate dehydrogenases. Advances in Enzymology 1979, vol. 48:97. John Wiley and Sons, New York.
37. Wenderoth I., Scheibe R., von Schaewen A. Identification of the cysteine residues involved in redox modification of plant plastidial glucose-6-phosphate dehydrogenase. J.Biol. Chem. 1997, 272:26985-26990.
38. Bradford M.M. Arapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976, 72:248-254.