Molecular characterization of a novel glucose-6-phosphate dehydrogenase from potato (Solanum tuberosum L.)

Plant Journal

Volumn 23, Issue 6, 2000, Pages 723-733

  Wendt, Urte Kristina ^a,b   Wenderoth, Irina ^a,b   Tegeler, Achim ^a   Von Schaewen, Antje ^a  

^a UNIVERSITY OF OSNABRÜCK   (Germany)

^b MPB Cologne GmbH   (Germany)

Author keywords

Expression; Glucose 6 phosphate dehydrogenase; Isoenzymes; Kinetic parameters; Plastidic; Redox regulation

Indexed keywords

AMINO ACID SEQUENCE; CHLOROPLAST; COMPLEMENTARY DNA; CYSTEINE; DITHIOTHREITOL; ENZYME ACTIVITY; ENZYME LOCALIZATION; ESCHERICHIA COLI; GLUCOSE 6 PHOSPHATE DEHYDROGENASE; GREEN FLUORESCENT PROTEIN; MESSENGER RNA; SEQUENCE HOMOLOGY; SOLANUM TUBEROSUM; THIOREDOXIN;

AMINO ACID SEQUENCE; BLOTTING, WESTERN; CLONING, MOLECULAR; DNA, COMPLEMENTARY; GLUCOSEPHOSPHATE DEHYDROGENASE; KINETICS; MOLECULAR SEQUENCE DATA; RECOMBINANT PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID; SOLANUM TUBEROSUM;

EID: 0033805886     PISSN: 09607412     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-313X.2000.00840.x     Document Type: Article

References (31)

1. Chloroplast protein topogenesis: Import, sorting and assembly
2. Nitrite reduction and carbohydrate metabolism in plastids purified from roots of Pisum sativum L
3. Reductant for glutamate synthase is generated by the oxidative pentose phosphate pathway in non-photosynthetic root plastids
4. 2 assimilation in oxygenic photosynthesis: The ferredoxin/thioredoxin system
5. The regulation of glycolysis and the pentose-phosphate pathway
6. Efficient transformation of Arabidopsis thaliana using direct gene transfer to protoplasts
7. Compartmentation of non-photosynthetic carbohydrate metabolism
8. Metabolism and transport in non-photosynthetic plastids
9. A conserved cleavage-site motif in chloroplast transit peptides
10. Purification, characterization and cDNA sequence of glucose-6-phosphate dehydrogenase from potato (Solanum tuberosum L.)
11. A one tube reaction for the synthesis of blunt-ended double-stranded cDNA
12. Dithiotreitol: An inhibitor of glucose-6-phosphate dehydrogenase activity in leaf extracts and isolated chloroplasts
13. A GFP-mouse talin fusion protein labels plant actin filaments in vivo and visualizes the actin cytosceleton in growing pollen tubes
14. Modulation of glucose-6-phosphate dehydrogenase by NADPH, NADP+ and dithiothreitol at variable NADPH/NADP+ ratios in an illuminated reconstituted spinach (Spinacia oleracea L.) chloroplast system
15. Improved method for the isolation of RNA from plant tissue
16. Purification of highly intact plastids from various heterotrophic plant tissues: Analysis of enzymic equipment and precursor dependency for starch biosynthesis
17. Induction of hexose-phosphate translocator activity in spinach chloroplasts
18. Molecular characterization of the plastidic glucose-6-phosphate dehydrogenase from potato in comparison to its cytosolic counterpart
19. Chloroplast glucose-6-phosphate dehydrogenase: K(m) shift upon light modulation and reduction
20. Molecular interaction between the Strep-tag affinity peptide and its cognate target streptavidin
21. Two isoenzymes each of glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase in spinach leaves
22. Catalytic properties of glucose-6-phosphate dehydrogenase from pea leaves
23. Use of the tetracycline promotor for the tightly regulated production of a murine antibody fragment in Escheridia coli
24. Mutagenesis of a flexible loop in streptavidin leads to higher affinity for the Strep-tag II peptide and improved performance in recombinant protein purification
25. Identification of the cysteine residues involved in redox modification of plant plastidic glucose-6-phosphate dehydrogenase
26. Evidence for functional convergence of redox regulation in G6PDH isoforms of cyanobacteria and higher plants
27. - assimilation