Human Colon Tissue in Organ Culture

Replacing Animal Models: A Practical Guide to Creating and Using Culture-Based Biomimetic Alternatives

Volumn , Issue , 2012, Pages 69-80

  Dame, Michael K ^a   Varani, James ^a  

^a UNIVERSITY OF MICHIGAN MEDICAL SCHOOL   (United States)

Author keywords

Colon cancer; Culture environment; Culture media; MMP 1 levels, in organ culture fluid; Normal human colonic epithelium; Organ culture method; Organ culture, and human colon tissue; Organ culture, biochemical features

Indexed keywords

EID: 84886120724     PISSN: None     EISSN: None     Source Type: Book    
DOI: 10.1002/9781119940685.ch7     Document Type: Chapter

References (45)

1. Whitfield JF. Calcium, calcium-sensing receptor and colon cancer. Cancer Lett. 2009;275:9-16.
2. Jemal A, Siegel R, Xu J, Ward E. Cancer statistics, 2010. CA Cancer J Clin. 2010;60:277-300.
3. Ferlay J, Shin HR, Bray F, Forman D, Mathers C, Parkin DM. Estimates of worldwide burden of cancer in 2008: GLOBOCAN 2008. Int J Cancer. 2010;127:2893-2917.
4. Takayama T, Ohi M, Hayashi T, et al. Analysis of Kras, APC, and beta-catenin in aberrant crypt foci in sporadic adenoma, cancer, and familial adenomatous polyposis. Gastroenterology. 2001;121:599-611.
5. Gupta AK, Pretlow TP, Schoen RE. Aberrant crypt foci: what we know and what we need to know. Clin Gastroenterol Hepatol. 2007;5:526-533.
6. Burgart LJ. Colorectal polyps and other precursor lesions. Need for an expanded view. Gastroenterol Clin North Am. 2002;31:959-970.
7. Taketo MM, Edelmann W. Mouse models of colon cancer. Gastroenterology. 2009;136:780-798.
8. Gouyer V, Fontaine D, Dumont P, et al. Autocrine induction of invasion and metastasis by tumorassociated trypsin inhibitor in human colon cancer cells. Oncogene. 2008;27:4024-4033.
9. Trier JS. Organ-culture methods in the study of gastrointestinal-mucosal function and development. N Engl J Med. 1976;295:150-155.
10. Autrup H, Barrett LA, Jackson FE, et al. Explant culture of human colon. Gastroenterology. 1978;74:1248-1257.
11. Kalus M. Carcinoma and adenomatous polyps of the colon and rectum in biopsy and organ tissue culture. Cancer. 1972;30:972-982.
12. Kolodkin-Gal D, Zamir G, Pikarski E, et al. A novel system to study adenovirus tropism to normal and malignant colon tissues. Virology. 2007;357:91-101.
13. Randall KJ, Turton J, Foster JR. Explant culture of gastrointestinal tissue: a review of methods and applications. Cell Biol Toxicol. 2011;27:267-84.
14. Dame MK, Bhagavathula N, Mankey C, et al. Human colon tissue in organ culture: preservation of normal and neoplastic characteristics. In Vitro Cell Dev Biol Anim. 2010;46:114-122.
15. DaSilva M, O'Brien Deming M, Fligiel SE, et al. Responses of human skin in organ culture and human skin fibroblasts to a gadolinium-based MRI contrast agent: comparison of skin from patients with endstage renal disease and skin from healthy subjects. Invest Radiol. 2010;45:733-739.
16. Varani J, Perone P, Spahlinger DM, et al. Human skin in organ culture and human skin cells (keratinocytes and fibroblasts) in monolayer culture for assessment of chemically induced skin damage. Toxicol Pathol. 2007;35:693-701.
17. Dame MK, Paruchuri T, DaSilva M, Bhagavathula N, Ridder W, Varani J. The Gottingen minipig for assessment of retinoid efficacy in the skin: comparison of results from topically treated animals with results from organ-cultured skin. In Vitro Cell Dev Biol Anim. 2009;45:551-557.
18. Varani J, Hattori Y, Chi Y, et al. Collagenolytic and gelatinolytic matrix metalloproteinases and their inhibitors in basal cell carcinoma of skin: comparison with normal skin. Br J Cancer. 2000;82:657-665.
19. Varani J, Hattori Y, Dame MK, et al. Matrix metalloproteinases (MMPs) in fresh human prostate tumour tissue and organ-cultured prostate tissue: levels of collagenolytic and gelatinolytic MMPs are low, variable and different in fresh tissue versus organ-cultured tissue. Br J Cancer. 2001;84:1076-1083.
20. Resau JH, Sakamoto K, Cottrell JR, Hudson EA, Meltzer SJ. Explant organ culture: a review. Cytotechnology. 1991;7:137-149.
21. Browning TH, Trier JS. Organ culture of mucosal biopsies of human small intestine. J Clin Invest. 1969;48:1423-1432.
22. Varani J, Larson BK, Perone P, Inman DR, Fligiel SE, Voorhees JJ. All-trans retinoic acid and extracellular Ca2+ differentially influence extracellular matrix production by human skin in organ culture. Am J Pathol 1993;142:1813-1822.
23. Varani J, Perone P, Griffiths CE, Inman DR, Fligiel SE, Voorhees JJ. All-trans retinoic acid (RA) stimulates events in organ-cultured human skin that underlie repair. Adult skin from sun-protected and sunexposed sites responds in an identical manner to RA while neonatal foreskin responds differently. J Clin Invest 1994;94:1747-1756.
24. Guarner F, Malagelada JR. Gut flora in health and disease. Lancet. 2003;361:512-519.
25. Dame MK, Spahlinger DM, DaSilva M, Perone P, Dunstan R, Varani J. Establishment and characteristics of Gottingen minipig skin in organ culture and monolayer cell culture: relevance to drug safety testing. In Vitro Cell Dev Biol Anim. 2008;44:245- 252.
26. Kesisoglou F, Schmiedlin-Ren P, Fleisher D, Roessler B, Zimmermann EM. Restituting intestinal epithelial cells exhibit increased transducibility by adenoviral vectors. J Gene Med; 2006;8:1379-1392.
27. Schmiedlin-Ren P, Benedict PE, Dobbins WO, 3rd, Ghosh M, Kolars JC, Watkins PB. Cultured adult rat jejunal explants as a model for studying regulation of CYP3A. Biochem Pharmacol. 1993;46:905-918.
28. Moorghen M, Chapman M, Appleton DR. An organculture method for human colorectal mucosa using serum-free medium. J Pathol. 1996;180:102-105.
29. Ahearn TU, McCullough ML, Flanders WD, et al. A randomized clinical trial of the effects of supplemental calcium and vitamin D3 on markers of their metabolism in normal mucosa of colorectal adenoma patients. Cancer Res. 2011;71:413-423.
30. Baron JA, Beach M, Mandel JS, et al. Calcium supplements for the prevention of colorectal adenomas. Calcium Polyp Prevention Study Group. N Engl J Med. 1999;340:101-107.
31. Grau MV, Baron JA, Sandler RS, et al. Prolonged effect of calcium supplementation on risk of colorectal adenomas in a randomized trial. J Natl Cancer Inst. 2007;99:129-136.
32. Beaty MM, Lee EY, Glauert HP. Influence of dietary calcium and vitamin D on colon epithelial cell proliferation and 1,2-dimethylhydrazine-induced colon carcinogenesis in rats fed high fat diets. J Nutr. 1993;123:144-152.
33. Bostick RM, Potter JD, Sellers TA, McKenzie DR, Kushi LH, Folsom AR. Relation of calcium, vitamin D, and dairy food intake to incidence of colon cancer among older women. The Iowa Women's Health Study. Am J Epidemiol. 1993;137:1302-1317.
34. Kampman E, Giovannucci E, van't Veer P, et al. Calcium, vitamin D, dairy foods, and the occurrence of colorectal adenomas among men and women in two prospective studies. Am J Epidemiol. 1994;139: 16-29.
35. Kampman E, Slattery ML, Caan B, Potter JD. Calcium, vitamin D, sunshine exposure, dairy products and colon cancer risk (United States). Cancer Causes Control. 2000;11:459-466.
36. Lamprecht SA, Lipkin M. Chemoprevention of colon cancer by calcium, vitamin D and folate: molecular mechanisms. Nat Rev Cancer. 2003;3:601-614.
37. Dame MK, Veerapaneni I, Bhagavathula N, Naik M, Varani J. Human colon tissue in organ culture: calcium and multi-mineral-induced mucosal differentiation. In Vitro Cell Dev Biol Anim. 2011;47:32-38.
38. Dionne S, Laberge S, Deslandres C, Seidman EG. Modulation of cytokine release from colonic explants by bacterial antigens in inflammatory bowel disease. Clin Exp Immunol. 2003;133:108-114.
39. Jarry A, Bossard C, Bou-Hanna C, Masson D, Espaze E, Denis MG, Laboisse CL. Mucosal IL-10 and TGFbeta play crucial roles in preventing LPS-driven, IFNgamma- mediated epithelial damage in human colon explants. J Clin Invest. 2008;118:1132-1142.
40. Autrup H. Explant culture of human colon. Methods Cell Biol. 1980;21B:385-401.
41. Senior PV, Pritchett CJ, Sunter JP, Appleton DR, Watson AJ. Crypt regeneration in adult human colonic mucosa during prolonged organ culture. J Anat. 1982;134:459-469.
42. Lipkin M. Growth and development of gastrointestinal cells. Annu Rev Physiol. 1985;47:175-197.
43. Baten A, Sakamoto K, Shamsuddin AM. Long-term culture of normal human colonic epithelial cells in vitro. FASEB J. 1992;6:2726-2734.
44. Whitehead RH, Demmler K, Rockman SP, Watson NK. Clonogenic growth of epithelial cells from normal colonic mucosa from both mice and humans. Gastroenterology. 1999;117:858-865.
45. Grossmann J, Walther K, Artinger M, et al. Progress on isolation and short-term ex-vivo culture of highly purified non-apoptotic human intestinal epithelial cells (IEC). Eur J Cell Biol. 2003;82:262-270.