Rapid identification of pathogens with the hemoFISH test applying a novel beacon-based fluorescence in situ hybridization (bbFISH) technology in positive blood culture bottles

Journal of Molecular Diagnostics

Volumn 15, Issue 6, 2013, Pages 835-839

  Leitner, Eva ^a   Scherr, Sabrina ^b   Strempfl, Christina ^a   Krause, Robert ^a   Feierl, Gebhard ^a   Grisold, Andrea J ^a  

^a MEDICAL UNIVERSITY OF GRAZ   (Austria)

^b University of Applied Sciences   (Austria)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; BLOOD CULTURE; DIAGNOSTIC ACCURACY; FLUORESCENCE IN SITU HYBRIDIZATION; GRAM NEGATIVE BACTERIUM; GRAM STAINING; HEMOFISH TEST; HUMAN; MICROBIOLOGICAL EXAMINATION; NONHUMAN; PATHOGENESIS; SEPSIS;

BACTERIAL TYPING TECHNIQUES; CULTURE TECHNIQUES; DNA PROBES; FLUORESCENT DYES; GRAM-NEGATIVE BACTERIA; GRAM-POSITIVE BACTERIA; HUMANS; IN SITU HYBRIDIZATION, FLUORESCENCE; SENSITIVITY AND SPECIFICITY; SEPSIS;

EID: 84886048554     PISSN: 15251578     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jmoldx.2013.07.007     Document Type: Article

References (21)

1. R.C. Bone, R.A. Balk, F.B. Cerra, R.P. Dellinger, A.M. Fein, W.A. Knaus, R.M. Schein, and W.J. Sibbald Definitions for sepsis and organ failure and guidelines for the use of innovative therapies in sepsis. The ACCP/SCCM Consensus Conference Committee American College of Chest Physicians/Society of Critical Care Medicine Chest 101 1992 1644 1655
2. J.E. Parrillo, M.M. Parker, C. Natanson, A.F. Suffredini, R.L. Danner, R.E. Cunnion, and F.P. Ognibene Septic shock in humans. Advances in the understanding of pathogenesis, cardiovascular dysfunction, and therapy Ann Intern Med 113 1990 227 242
3. A. Kumar, D. Roberts, K.E. Wood, B. Light, J.E. Parrillo, S. Sharma, R. Suppes, D. Feinstein, S. Zanotti, L. Taiberg, D. Gurka, A. Kumar, and M. Cheang Duration of hypotension before initiation of effective antimicrobial therapy is the critical determinant of survival in human septic shock Crit Care Med 34 2006 1589 1596
4. R.P. Dellinger, J.M. Carlet, H. Masur, H. Gerlach, T. Calandra, J. Cohen, J. Gea-Banacloche, D. Keh, J.C. Marshall, M.M. Parker, G. Ramsay, J.L. Zimmerman, J.L. Vincent, M.M. Levy Surviving Sepsis Campaign Management Guidelines Committee Surviving Sepsis Campaign guidelines for management of severe sepsis and septic shock Crit Care Med 32 2004 858 873
5. M.H. Kollef Inadequate antimicrobial treatment: an important determinant of outcome for hospitalized patients Clin Infect Dis 31 suppl 4 2000 S131 S138
6. R.P. Peters, M.A. van Agtmael, S.A. Danner, P.H. Savelkoul, and C.M. Vandenbroucke-Grauls New developments in the diagnosis of bloodstream infections Lancet Infect Dis 4 2004 751 760
7. T.J. Kirn, and M.P. Weinstein Update on blood cultures: how to obtain, process, report, and interpret Clin Microbiol Infect 19 2013 513 520
8. V.A. Kempf, K. Trebesius, and I.B. Autenrieth Fluorescent in situ hybridization allows rapid identification of microorganisms in blood cultures J Clin Microbiol 38 2000 830 838
9. H. Stender PNA FISH: an intelligent stain for rapid diagnosis of infectious diseases Expert Rev Mol Diagn 3 2003 649 655
10. V. Gonzalez, E. Padilla, M. Gimenez, C. Vilaplana, A. Perez, G. Fernandez, M.D. Quesada, M.A. Pallares, and V. Ausina Rapid diagnosis of Staphylococcus aureus bacteremia using S. aureus PNA FISH Eur J Clin Microbiol Infect Dis 23 2004 396 398
11. P. Della-Latta, H. Salimnia, T. Painter, F. Wu, G.W. Procop, D.A. Wilson, W. Gillespie, A. Mender, and B.S. Crystal Identification of Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa in blood cultures: a multicenter performance evaluation of a three-color peptide nucleic acid fluorescence in situ hybridization assay J Clin Microbiol 49 2011 2259 2261
12. R.P. Peters, M.A. van Agtmael, A.M. Simoons-Smit, S.A. Danner, C.M. Vandenbroucke-Grauls, and P.H. Savelkoul Rapid identification of pathogens in blood cultures with a modified fluorescence in situ hybridization assay J Clin Microbiol 44 2006 4186 4188
13. T. Antony, and V. Subramaniam Molecular beacons: nucleic acid hybridization and emerging applications J Biomol Struct Dyn 19 2001 497 504
14. H.J. Tanke, R.W. Dirks, and T. Raap FISH and immunocytochemistry: towards visualising single target molecules in living cells Curr Opin Biotechnol 16 2005 49 54
15. D. Annane, E. Bellissant, and J.M. Cavaillon Septic shock Lancet 365 2005 63 78
16. T. Hautala, H. Syrjala, V. Lehtinen, H. Kauma, J. Kauppila, P. Kujala, I. Pietarinen, P. Ylipalosaari, and M. Koskela Blood culture Gram stain and clinical categorization based empirical antimicrobial therapy of bloodstream infection Int J Antimicrob Agents 25 2005 329 333
17. K.K. Hall, and J.A. Lyman Updated review of blood culture contamination Clin Microbiol Rev 19 2006 788 802
18. M.K. Deck, E.S. Anderson, R.J. Buckner, G. Colasante, J.M. Coull, B. Crystal, P. Della Latta, M. Fuchs, D. Fuller, W. Harris, K. Hazen, L.L. Klimas, D. Lindao, M.C. Meltzer, M. Morgan, J. Shepard, S. Stevens, F. Wu, and M.J. Fiandaca Multicenter evaluation of the Staphylococcus QuickFISH method for simultaneous identification of Staphylococcus aureus and coagulase-negative staphylococci directly from blood culture bottles in less than 30 minutes J Clin Microbiol 50 2012 1994 1998
19. S. Poppert, M. Riecker, N. Wellinghausen, H. Frickmann, and A. Essig Accelerated identification of Staphylococcus aureus from blood cultures by a modified fluorescence in situ hybridization procedure J Med Microbiol 59 2010 65 68
20. M.A. Morgan, E. Marlowe, S. Novak-Weekly, J.M. Miller, T.M. Painter, H. Salimnia, and B. Crystal A 1.5 hour procedure for identification of Enterococcus Species directly from blood cultures J Vis Exp 2011 (48). pii: 2616
21. M. Sogaard, H. Stender, and H.C. Schonheyder Direct identification of major blood culture pathogens, including Pseudomonas aeruginosa and Escherichia coli, by a panel of fluorescence in situ hybridization assays using peptide nucleic acid probes J Clin Microbiol 43 2005 1947 1949