Accelerated identification of Staphylococcus aureus from blood cultures by a modified fluorescence in situ hybridization procedure

Journal of Medical Microbiology

Volumn 59, Issue 1, 2010, Pages 65-68

  Poppert, Sven ^a   Riecker, Melanie ^b   Wellinghausen, Nele ^b   Frickmann, Hagen ^c   Essig, Andreas ^b  

^a BERNHARD NOCHT INSTITUTE FOR TROPICAL MEDICINE   (Germany)

^b UNIVERSITY OF ULM   (Germany)

^c UNIVERSITY OF ROSTOCK   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; BACTERIUM IDENTIFICATION; BLOOD CULTURE; COAGULASE NEGATIVE STAPHYLOCOCCUS; FLUORESCENCE IN SITU HYBRIDIZATION; LABORATORY; MICROWAVE RADIATION; NONHUMAN; PRIORITY JOURNAL; RELIABILITY; STAPHYLOCOCCUS AUREUS;

BACTERIOLOGICAL TECHNIQUES; HUMANS; IN SITU HYBRIDIZATION, FLUORESCENCE; MICROWAVES; STAPHYLOCOCCAL INFECTIONS; STAPHYLOCOCCUS AUREUS;

EID: 73649120596     PISSN: 00222615     EISSN: None     Source Type: Journal    
DOI: 10.1099/jmm.0.015503-0     Document Type: Article

References (22)

1. Amann, R. I., Binder, B. J., Olson, R. J., Chisholm, S. W., Devereux, R. & Stahl, D. A. (1990). Combination of 16S rRNA-targeted oligonucleotide probes with flow cytometry for analyzing mixed microbial populations. Appl Environ Microbiol 56, 1919-1925.
2. Barenfanger, J., Drake, C. & Kacich, G. (1999). Clinical and financial benefits of rapid bacterial identification and antimicrobial susceptibility testing. J Clin Microbiol 37, 1415-1418.
3. Chapin, K. & Musgnug, M. (2003). Evaluation of three rapid methods for the direct identification of Staphylococcus aureus from positive blood cultures. J Clin Microbiol 41, 4324-4327.
4. Forrest, G. N., Mehta, S., Weekes, E., Lincalis, D. P., Johnson, J. K. & Venezia, R. A. (2006). Impact of rapid in situ hybridization testing on coagulase-negative staphylococci positive blood cultures. J Antimicrob Chemother 58, 154-158.
5. Gescher, D. M., Kovacevic, D., Schmiedel, D., Siemoneit, S., Mallmann, C., Halle, E., Göbel, U. B. & Moter, A. (2008). Fluorescence in situ hybridisation (FISH) accelerates identification of Gram-positive cocci in positive blood cultures. Int J Antimicrob Agents 32 (Suppl. 1), S51-S59.
6. González, V., Padilla, E., Gimenez, M., Vilaplana, C., Pérez, A., Fernández, G., Quesada, M. D., Pallarés, M. A. & Ausina, V. (2004). Rapid diagnosis of Staphylococcus aureus bacteremia using S. aureus PNA FISH. Eur J Clin Microbiol Infect Dis 23, 396-398.
7. Hartmann, H., Stender, H., Schafer, A., Autenrieth, I. B. & Kempf, V. A. (2005). Rapid identification of Staphylococcus aureus in blood cultures by a combination of fluorescence in situ hybridization using peptide nucleic acid probes and flow cytometry. J Clin Microbiol 43, 4855-4857.
8. Hensley, D. M., Tapia, R. & Encina, Y. (2009). An evaluation of the AdvanDx Staphylococcus aureus/CNS PNA FISH assay. Clin Lab Sci 22, 30-33.
9. Hiraishi, A. (1992). Direct automated sequencing of 16S rDNA amplified by polymerase chain reaction from bacterial cultures without DNA purification. Lett Appl Microbiol 15, 210-213.
10. Jansen, G. J., Mooibroek, M., Idema, J., Harmsen, H. J., Welling, G. W. & Degener, J. E. (2000). Rapid identification of bacteria in blood cultures by using fluorescently labeled oligonucleotide probes. J Clin Microbiol 38, 814-817.
11. Kempf, V. A., Trebesius, K. & Autenrieth, I. B. (2000). Fluorescent in situ hybridization allows rapid identification of microorganisms in blood cultures. J Clin Microbiol 38, 830-838.
12. Ly, T., Gulia, J., Pyrgos, V., Waga, M. & Shoham, S. (2008). Impact upon clinical outcomes of translation of PNA FISH-generated laboratory data from the clinical microbiology bench to bedside in real time. Ther Clin Risk Manag 4, 637-640.
13. Mehta, M. S., Paule, S. M., Thomson, R. B., Kaul, K. L. & Peterson, L. R. (2009). Identification of Staphylococcus species directly from positive blood culture broth by use of molecular and conventional methods. J Clin Microbiol 47, 1082-1086.
14. Moter, A. & Göbel, U. B. (2000). Fluorescence in situ hybridization (FISH) for direct visualization of microorganisms. J Microbiol Methods 41, 85-112.
15. Oliveira, K., Procop, G. W., Wilson, D., Coull, J. & Stender, H. (2002). Rapid identification of Staphylococcus aureus directly from blood cultures by fluorescence in situ hybridization with peptide nucleic acid probes. J Clin Microbiol 40, 247-251.
16. Oliveira, K., Brecher, S. M., Durbin, A., Shapiro, D. S., Schwartz, D. R., De Girolami, P. C., Dakos, J., Procop, G. W., Wilson, D. & other authors (2003). Direct identification of Staphylococcus aureus from positive blood culture bottles. J Clin Microbiol 41, 889-891.
17. Peters, R. P., van Agtmael, M. A., Simoons-Smit, A. M., Danner, S. A., Vandenbroucke-Grauls, C. M. & Savelkoul, P. H. (2006). Rapid identification of pathogens in blood cultures with a modified fluorescence in situ hybridization assay. J Clin Microbiol 44, 4186-4188.
18. Ruimy, R., Dos-Santos, M., Raskine, L., Bert, F., Masson, R., Elbaz, S., Bonnal, C., Lucet, J. C., Lefort, A. & other authors (2008). Accuracy and potential usefulness of triplex real-time PCR for improving antibiotic treatment of patients with blood cultures showing clustered Gram-positive cocci on direct smears. J Clin Microbiol 46, 2045-2051.
19. Speers, D. J., Olma, T. R. & Gilbert, G. L. (1998). Evaluation of four methods for rapid identification of Staphylococcus aureus from blood cultures. J Clin Microbiol 36, 1032-1034.
20. Tavares, A., Inacio, J., Melo-Cristino, J. & Couto, I. (2008). Use of fluorescence in situ hybridization for rapid identification of staphylococci in blood culture samples collected in a Portuguese hospital. J Clin Microbiol 46, 3097-3100.
21. Weinstein, M. P. (2003). Blood culture contamination: persisting problems and partial progress. J Clin Microbiol 41, 2275-2278.
22. Wellinghausen, N., Bartel, M., Essig, A. & Poppert, S. (2007). Rapid identification of clinically relevant Enterococcus species by fluorescence in situ hybridization. J Clin Microbiol 45, 3424-3426.