Gas chromatography-mass spectrometry method for rapid identification and differentiation of Burkholderia pseudomallei and Burkholderia mallei from each other, Burkholderia thailandensis and several members of the Burkholderia cepacia complex

Journal of Applied Microbiology

Volumn 115, Issue 5, 2013, Pages 1159-1171

  Li, D ^a   March, J K ^b   Bills, T M ^b   Holt, B C ^b   Wilson, C E ^a   Lowe, W ^b   Tolley, H D ^b   Lee, M L ^a   Robison, R A ^b  

^a Brigham Young University   (United States)

^b BRIGHAM YOUNG UNIVERSITY   (United States)

Author keywords

Burkholderia mallei; Burkholderia pseudomallei; Differentiation; Identification; Thermochemolysis

Indexed keywords

FATTY ACIDS; GAS CHROMATOGRAPHY; MASS SPECTROMETRY; STRAIN;

BURKHOLDERIA; BURKHOLDERIA CEPACIA COMPLEX; BURKHOLDERIA MALLEUS; BURKHOLDERIA PSEUDOMALLEI; DIFFERENTIATION; GAS CHROMATOGRAPHY-MASS SPECTROMETRY METHODS; IDENTIFICATION; RAPID DIFFERENTIATION; RAPID IDENTIFICATION; THERMOCHEMOLYSIS;

BIOMARKERS;

FATTY ACID ESTER; POLY 3 HYDROXYBUTYRATE CO HYDROXYVALERATE; POLY(3 HYDROXYBUTYRIC ACID); UNCLASSIFIED DRUG;

ALGORITHM; BACTERIUM; BIOASSAY; BIOMARKER; FATTY ACID; GAS CHROMATOGRAPHY; MASS SPECTROMETRY; TEMPERATURE EFFECT;

ACCURACY; ALGORITHM; ANALYTIC METHOD; ARTICLE; BACTERIAL CELL; BACTERIAL GROWTH; BACTERIAL STRAIN; BACTERIUM DETECTION; BACTERIUM IDENTIFICATION; BURKHOLDERIA; BURKHOLDERIA CEPACIA; BURKHOLDERIA CEPACIA COMPLEX; BURKHOLDERIA MALLEI; BURKHOLDERIA MULTIVORANS; BURKHOLDERIA PSEUDOMALLEI; BURKHOLDERIA THAILANDENSIS; BURKHOLDERIA VIETNAMIENSIS; LIMIT OF DETECTION; MASS FRAGMENTOGRAPHY; SPECIES DIFFERENTIATION; THERMOCHEMOLYSIS;

BURKHOLDERIA; BURKHOLDERIA CEPACIA COMPLEX; BURKHOLDERIA MALLEI; BURKHOLDERIA PSEUDOMALLEI; BURKHOLDERIA THAILANDENSIS;

BURKHOLDERIA MALLEI; BURKHOLDERIA PSEUDOMALLEI; DIFFERENTIATION; IDENTIFICATION; THERMOCHEMOLYSIS;

ALGORITHMS; BIOLOGICAL MARKERS; BURKHOLDERIA CEPACIA COMPLEX; BURKHOLDERIA MALLEI; BURKHOLDERIA PSEUDOMALLEI; FATTY ACIDS; GAS CHROMATOGRAPHY-MASS SPECTROMETRY; POLYESTERS;

EID: 84885836295     PISSN: 13645072     EISSN: 13652672     Source Type: Journal    
DOI: 10.1111/jam.12310     Document Type: Article

References (69)

1. Amornchai, P., Chierakul, W., Wuthiekanun, V., Mahakhunkijcharoen, Y., Phetsouvanh, R., Currie, B.J., Newton, P.N., van Vinh Chau, N. et al. (2007) Accuracy of Burkholderia pseudomallei identification using the API 20NE system and a latex agglutination test. J Clin Microbiol 45, 3774-3776.
2. Antolin, E.M., Delange, D.M. and Canavaciolo, V.G. (2008) Evaluation of five methods for derivatization and GC determination of a mixture of very long chain fatty acids (C24:0-C36:0). J Pharm Biomed Anal 46, 194-199.
3. Anuntagool, N., Naigowit, P., Petkanchanapong, V., Aramsri, P., Panichakul, T. and Sirisinha, S. (2000) Monoclonal antibody-based rapid identification of Burkholderia pseudomallei in blood culture fluid from patients with community-acquired septicaemia. J Med Microbiol 49, 1075-1078.
4. Bauernfeind, A., Schneider, I., Jungwirth, R. and Roller, C. (1999) Discrimination of Burkholderia multivorans and Burkholderia vietnamiensis from Burkholderia cepacia genomovars I, III, and IV by PCR. J Clin Microbiol 37, 1335-1339.
5. Bowers, J.R., Engelthaler, D.M., Ginther, J.L., Pearson, T., Peacock, S.J., Tuanyok, A., Wagner, D.M., Currie, B.J. et al. (2010) BurkDiff: a real-time PCR allelic discrimination assay for Burkholderia pseudomallei and B. mallei. PLoS ONE 5, e15413.
6. Brett, P.J., DeShazer, D. and Woods, D.E. (1998) Burkholderia thailandensis sp. nov., a Burkholderia pseudomallei-like species. Int J Syst Bacteriol 48 Pt 1, 317-320.
7. Brilhante, R.S., Bandeira, T.J., Cordeiro, R.A., Grangeiro, T.B., Lima, R.A., Ribeiro, J.F., Castelo-Branco, D.S., Rodrigues, J.L. et al. (2012) Clinical-epidemiological features of 13 cases of melioidosis in Brazil. J Clin Microbiol 50, 3349-3352.
8. Chantratita, N., Meumann, E., Thanwisai, A., Limmathurotsakul, D., Wuthiekanun, V., Wannapasni, S., Tumapa, S., Day, N.P. et al. (2008) Loop-mediated isothermal amplification method targeting the TTS1 gene cluster for detection of Burkholderia pseudomallei and diagnosis of melioidosis. J Clin Microbiol 46, 568-573.
9. Chaowagul, W., White, N.J., Dance, D.A., Wattanagoon, Y., Naigowit, P., Davis, T.M., Looareesuwan, S. and Pitakwatchara, N. (1989) Melioidosis: a major cause of community-acquired septicemia in northeastern Thailand. J Infect Dis 159, 890-899.
10. Chen, Y.S., Lin, H.H., Mu, J.J., Chiang, C.S., Chen, C.H., Buu, L.M., Lin, Y.E. and Chen, Y.L. (2010) Distribution of melioidosis cases and viable Burkholderia pseudomallei in soil: evidence for emerging melioidosis in Taiwan. J Clin Microbiol 48, 1432-1434.
11. Cheng, A.C. and Currie, B.J. (2005) Melioidosis: epidemiology, pathophysiology, and management. Clin Microbiol Rev 18, 383-416.
12. Cheng, A.C., O'brien, M., Freeman, K., Lum, G. and Currie, B.J. (2006) Indirect hemagglutination assay in patients with melioidosis in northern Australia. Am J Trop Med Hyg 74, 330-334.
13. Chenthamarakshan, V., Vadivelu, J. and Puthucheary, S.D. (2001) Detection of immunoglobulins M and G using culture filtrate antigen of Burkholderia pseudomallei. Diagn Microbiol Infect Dis 39, 1-7.
14. Currie, B.J., Fisher, D.A., Howard, D.M., Burrow, J.N., Lo, D., Selva-Nayagam, S., Anstey, N.M., Huffam, S.E. et al. (2000) Endemic melioidosis in tropical northern Australia: a 10-year prospective study and review of the literature. Clin Infect Dis 31, 981-986.
15. Currie, B.J., Dance, D.A. and Cheng, A.C. (2008) The global distribution of Burkholderia pseudomallei and melioidosis: an update. Trans R Soc Trop Med Hyg 102(Suppl 1), S1-S4.
16. Dharakul, T., Tassaneetrithep, B., Trakulsomboon, S. and Songsivilai, S. (1999) Phylogenetic analysis of Ara+ and Ara- Burkholderia pseudomallei isolates and development of a multiplex PCR procedure for rapid discrimination between the two biotypes. J Clin Microbiol 37, 1906-1912.
17. Fredricks, D.N. and Relman, D.A. (1999) Application of polymerase chain reaction to the diagnosis of infectious diseases. Clin Infect Dis 29, 475-486; quiz 487-8.
18. Gee, J.E., Sacchi, C.T., Glass, M.B., De, B.K., Weyant, R.S., Levett, P.N., Whitney, A.M., Hoffmaster, A.R. et al. (2003) Use of 16S rRNA gene sequencing for rapid identification and differentiation of Burkholderia pseudomallei and B. mallei. J Clin Microbiol 41, 4647-4654.
19. Glass, M.B. and Popovic, T. (2005) Preliminary evaluation of the API 20NE and RapID NF plus systems for rapid identification of Burkholderia pseudomallei and B. mallei. J Clin Microbiol 43, 479-483.
20. Glass, M.B., Gee, J.E., Steigerwalt, A.G., Cavuoti, D., Barton, T., Hardy, R.D., Godoy, D., Spratt, B.G. et al. (2006) Pneumonia and septicemia caused by Burkholderia thailandensis in the United States. J Clin Microbiol 44, 4601-4604.
21. Godoy, D., Randle, G., Simpson, A.J., Aanensen, D.M., Pitt, T.L., Kinoshita, R. and Spratt, B.G. (2003) Multilocus sequence typing and evolutionary relationships among the causative agents of melioidosis and glanders, Burkholderia pseudomallei and Burkholderia mallei. J Clin Microbiol 41, 2068-2079.
22. Gregory, B.C. and Waag, D.M. (2007). Glanders. In Military Medicine ed. Dembek Z.F. pp. 121-146. Washington, D.C.: Department of Defense, Office of The Surgeon General, US Army, Borden Institute.
23. Hagen, R.M., Frickmann, H., Elschner, M., Melzer, F., Neubauer, H., Gauthier, Y.P., Racz, P. and Poppert, S. (2011) Rapid identification of Burkholderia pseudomallei and Burkholderia mallei by fluorescence in situ hybridization (FISH) from culture and paraffin-embedded tissue samples. Int J Med Microbiol 301, 585-590.
24. Howe, C., Sampath, A. and Spotnitz, M. (1971) The pseudomallei group: a review. J Infect Dis 124, 598-606.
25. Inglis, T.J., Chiang, D., Lee, G.S. and Chor-Kiang, L. (1998) Potential misidentification of Burkholderia pseudomallei by API 20NE. Pathology 30, 62-64.
26. Inglis, T.J., Aravena-Roman, M., Ching, S., Croft, K., Wuthiekanun, V. and Mee, B.J. (2003) Cellular fatty acid profile distinguishes Burkholderia pseudomallei from avirulent Burkholderia thailandensis. J Clin Microbiol 41, 4812-4814.
27. Inglis, T.J., Merritt, A., Chidlow, G., Aravena-Roman, M. and Harnett, G. (2005) Comparison of diagnostic laboratory methods for identification of Burkholderia pseudomallei. J Clin Microbiol 43, 2201-2206.
28. John, T.J., Jesudason, M.V., Lalitha, M.K., Ganesh, A., Mohandas, V., Cherian, T., Mathai, D. and Chandy, M.J. (1996) Melioidosis In India: the tip of the iceberg? Indian J Med Res 103, 62-65.
29. Kim do, Y., Park, D.S., Kwon, S.B., Chung, M.G., Bae, K.S., Park, H.Y. and Rhee, Y.H. (2009) Biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) copolyesters with a high molar fraction of 3-hydroxyvalerate by an insect-symbiotic Burkholderia sp. IS-01. J Microbiol 47, 651-656.
30. Kiska, D.L., Kerr, A., Jones, M.C., Caracciolo, J.A., Eskridge, B., Jordan, M., Miller, S., Hughes, D. et al. (1996) Accuracy of four commercial systems for identification of Burkholderia cepacia and other gram-negative nonfermenting bacilli recovered from patients with cystic fibrosis. J Clin Microbiol 34, 886-891.
31. Klein, D. (2002) Quantification using real-time PCR technology: applications and limitations. Trends Mol Med 8, 257-260.
32. Krejci, E. and Kroppenstedt, R.M. (2006) Differentiation of species combined into the Burkholderia cepacia complex and related taxa on the basis of their fatty acid patterns. J Clin Microbiol 44, 1159-1164.
33. Lee, M.A., Wang, D. and Yap, E.H. (2005) Detection and differentiation of Burkholderia pseudomallei, Burkholderia mallei and Burkholderia thailandensis by multiplex PCR. FEMS Immunol Med Microbiol 43, 413-417.
34. Lertpatanasuwan, N., Sermsri, K., Petkaseam, A., Trakulsomboon, S., Thamlikitkul, V. and Suputtamongkol, Y. (1999) Arabinose-positive Burkholderia pseudomallei infection in humans: case report. Clin Infect Dis 28, 927-928.
35. Levy, A., Merritt, A.J., Aravena-Roman, M., Hodge, M.M. and Inglis, T.J. (2008) Expanded range of Burkholderia species in Australia. Am J Trop Med Hyg 78, 599-604.
36. Li, D., Truong, T.V., Bills, T.M., Holt, B.C., VanDerwerken, D.N., Williams, J.R., Acharya, A., Robison, R.A. et al. (2012) GC/MS method for positive detection of Bacillus anthracis endospores. Anal Chem 84, 1637-1644.
37. Limmathurotsakul, D., Wongratanacheewin, S., Teerawattanasook, N., Wongsuvan, G., Chaisuksant, S., Chetchotisakd, P., Chaowagul, W., Day, N.P. et al. (2010) Increasing incidence of human melioidosis in Northeast Thailand. Am J Trop Med Hyg 82, 1113-1117.
38. Lipuma, J.J. (2005) Update on the Burkholderia cepacia complex. Curr Opin Pulm Med 11, 528-533.
39. Lowe, P., Engler, C. and Norton, R. (2002) Comparison of automated and nonautomated systems for identification of Burkholderia pseudomallei. J Clin Microbiol 40, 4625-4627.
40. Mahenthiralingam, E., Bischof, J., Byrne, S.K., Radomski, C., Davies, J.E., Av-Gay, Y. and Vandamme, P. (2000) DNA-Based diagnostic approaches for identification of Burkholderia cepacia complex, Burkholderia vietnamiensis, Burkholderia multivorans, Burkholderia stabilis, and Burkholderia cepacia genomovars I and III. J Clin Microbiol 38, 3165-3173.
41. McMenamin, J.D., Zaccone, T.M., Coenye, T., Vandamme, P. and LiPuma, J.J. (2000) Misidentification of Burkholderia cepacia in US cystic fibrosis treatment centers: an analysis of 1, 051 recent sputum isolates. Chest 117, 1661-1665.
42. Novak, R.T., Glass, M.B., Gee, J.E., Gal, D., Mayo, M.J., Currie, B.J. and Wilkins, P.P. (2006) Development and evaluation of a real-time PCR assay targeting the type III secretion system of Burkholderia pseudomallei. J Clin Microbiol 44, 85-90.
43. Novem, V., Shui, G., Wang, D., Bendt, A.K., Sim, S.H., Liu, Y., Thong, T.W., Sivalingam, S.P. et al. (2009) Structural and biological diversity of lipopolysaccharides from Burkholderia pseudomallei and Burkholderia thailandensis. Clin Vaccine Immunol 16, 1420-1428.
44. Phung, L.V., Tran, T.B., Hotta, H., Yabuuchi, E. and Yano, I. (1995) Cellular lipid and fatty acid compositions of Burkholderia pseudomallei strains isolated from human and environment in Viet Nam. Microbiol Immunol 39, 105-116.
45. Puthucheary, S.D., Puah, S.M., Chai, H.C., Thong, K.L. and Chua, K.H. (2012) Molecular investigation of virulence determinants between a virulent clinical strain and an attenuated strain of Burkholderia pseudomallei. J Mol Microbiol Biotechnol 22, 198-204.
46. Rogul, M., Brendle, J.J., Haapala, D.K. and Alexander, A.D. (1970) Nucleic acid similarities among Pseudomonas pseudomallei, Pseudomonas multivorans, and Actinobacillus mallei. J Bacteriol 101, 827-835.
47. Samosornsuk, N., Lulitanond, A., Saenla, N., Anuntagool, N., Wongratanacheewin, S. and Sirisinha, S. (1999) Short report: evaluation of a monoclonal antibody-based latex agglutination test for rapid diagnosis of septicemic melioidosis. Am J Trop Med Hyg 61, 735-737.
48. Sanford, J.P. (1995). Pseudomonas species (including melioidosis and glanders). In Mandell, Douglas, and Bennett's Principles and Practice of Infectious Diseases ed. Mandell, G.L., Bennett, J.E. and Dolin, R. pp. 2003-2009. New York: Churchill Livingstone.
49. Spickler, A.R. (2008). Glanders. In Handbook for Zoonotic Diseases of Companion Animals ed. Dvorak, D., Spickler, A.R. and Roth, J.A. pp. 133-135. Ames: Center for Food Security and Public Health.
50. Sprague, L.D., Zysk, G., Hagen, R.M., Meyer, H., Ellis, J., Anuntagool, N., Gauthier, Y. and Neubauer, H. (2002) A possible pitfall in the identification of Burkholderia mallei using molecular identification systems based on the sequence of the flagellin fliC gene. FEMS Immunol Med Microbiol 34, 231-236.
51. Steinbuchel, A. (1991) Recent advances in the knowledge of the metabolism of bacterial polyhydroxyalkanoic acids and potential impacts on the production of biodegradable thermoplastics. Acta Biotechnol 11, 419-427.
52. Steinbuchel, A. and Valentine, H.E. (1995) Diversity of bacterial polyhydroxyalkanoic acids. FEMS Microbiol Lett 128, 219-228.
53. Steinmetz, I., Reganzerowski, A., Brenneke, B., Haussler, S., Simpson, A. and White, N.J. (1999) Rapid identification of Burkholderia pseudomallei by latex agglutination based on an exopolysaccharide-specific monoclonal antibody. J Clin Microbiol 37, 225-228.
54. Suputtamongkol, Y., Hall, A.J., Dance, D.A., Chaowagul, W., Rajchanuvong, A., Smith, M.D. and White, N.J. (1994) The epidemiology of melioidosis in Ubon Ratchatani, northeast Thailand. Int J Epidemiol 23, 1082-1090.
55. Thibault, F.M., Valade, E. and Vidal, D.R. (2004) Identification and discrimination of Burkholderia pseudomallei, B. mallei, and B. thailandensis by real-time PCR targeting type III secretion system genes. J Clin Microbiol 42, 5871-5874.
56. U'Ren, J.M., Van Ert, M.N., Schupp, J.M., Easterday, W.R., Simonson, T.S., Okinaka, R.T., Pearson, T. and Keim, P. (2005) Use of a real-time PCR TaqMan assay for rapid identification and differentiation of Burkholderia pseudomallei and Burkholderia mallei. J Clin Microbiol 43, 5771-5774.
57. U'Ren, J.M., Schupp, J.M., Pearson, T., Hornstra, H., Friedman, C.L., Smith, K.L., Daugherty, R.R., Rhoton, S.D. et al. (2007) Tandem repeat regions within the Burkholderia pseudomallei genome and their application for high resolution genotyping. BMC Microbiol 7, 23.
58. Vandamme, P. and Dawyndt, P. (2011) Classification and identification of the Burkholderia cepacia complex: past, present and future. Syst Appl Microbiol 34, 87-95.
59. Vandamme, P., Pot, B., Gillis, M., de Vos, P., Kersters, K. and Swings, J. (1996) Polyphasic taxonomy, a consensus approach to bacterial systematics. Microbiol Rev 60, 407-438.
60. Walsh, A.L., Smith, M.D., Wuthiekanun, V., Suputtamongkol, Y., Desakorn, V., Chaowagul, W. and White, N.J. (1994) Immunofluorescence microscopy for the rapid diagnosis of melioidosis. J Clin Pathol 47, 377-379.
61. White, N.J. (2003) Melioidosis. Lancet 361, 1715-1722.
62. White, N.J., Dance, D.A., Chaowagul, W., Wattanagoon, Y., Wuthiekanun, V. and Pitakwatchara, N. (1989) Halving of mortality of severe melioidosis by ceftazidime. Lancet 2, 697-701.
63. Whitlock, G.C., Estes, D.M. and Torres, A.G. (2007) Glanders: off to the races with Burkholderia mallei. FEMS Microbiol Lett 277, 115-122.
64. Wongtrakoongate, P., Mongkoldhumrongkul, N., Chaijan, S., Kamchonwongpaisan, S. and Tungpradabkul, S. (2007) Comparative proteomic profiles and the potential markers between Burkholderia pseudomallei and Burkholderia thailandensis. Mol Cell Probes 21, 81-91.
65. Wuthiekanun, V., Amornchai, P., Chierakul, W., Cheng, A.C., White, N.J., Peacock, S.J. and Day, N.P. (2004) Evaluation of immunoglobulin M (IgM) and IgG rapid cassette test kits for diagnosis of melioidosis in an area of endemicity. J Clin Microbiol 42, 3435-3437.
66. Wuthiekanun, V., Desakorn, V., Wongsuvan, G., Amornchai, P., Cheng, A.C., Maharjan, B., Limmathurotsakul, D., Chierakul, W. et al. (2005) Rapid immunofluorescence microscopy for diagnosis of melioidosis. Clin Diagn Lab Immunol 12, 555-556.
67. Yabuuchi, E., Kosako, Y., Oyaizu, H., Yano, I., Hotta, H., Hashimoto, Y., Ezaki, T. and Arakawa, M. (1992) Proposal of Burkholderia gen. nov. and transfer of seven species of the genus Pseudomonas homology group II to the new genus, with the type species Burkholderia cepacia (Palleroni and Holmes 1981) comb. nov. Microbiol Immunol 36, 1251-1275.
68. Yang, S. (2000) Melioidosis research in China. Acta Trop 77, 157-165.
69. Yang, S. and Rothman, R.E. (2004) PCR-based diagnostics for infectious diseases: uses, limitations, and future applications in acute-care settings. Lancet Infect Dis 4, 337-348.