-
1
-
-
0032949461
-
Transcriptional regulation of molybdoenzyme synthesis in Escherichia coli in response to molybdenum: ModE-molybdate, a repressor of the modABCD (molybdate transport) operon is a secondary transcriptional activator for the hyc and nar operons
-
Self WT, Grunden AM, Hasona A, Shanmugam KT. 1999. Transcriptional regulation of molybdoenzyme synthesis in Escherichia coli in response to molybdenum: ModE-molybdate, a repressor of the modABCD (molybdate transport) operon is a secondary transcriptional activator for the hyc and nar operons. Microbiology 145:41-55.
-
(1999)
Microbiology
, vol.145
, pp. 41-55
-
-
Self, W.T.1
Grunden, A.M.2
Hasona, A.3
Shanmugam, K.T.4
-
2
-
-
0030028140
-
Repression of the Escherichia coli modABCD (molybdate transport) operon by ModE
-
Grunden AM, Ray RM, Rosentel JK, Healy FG, Shanmugam KT. 1996. Repression of the Escherichia coli modABCD (molybdate transport) operon by ModE. J. Bacteriol. 178:735-744.
-
(1996)
J. Bacteriol.
, vol.178
, pp. 735-744
-
-
Grunden, A.M.1
Ray, R.M.2
Rosentel, J.K.3
Healy, F.G.4
Shanmugam, K.T.5
-
3
-
-
0033559680
-
The high-resolution crystal structure of the molybdate-dependent transcriptional regulator (ModE) from Escherichia coli: a novel combination of domain folds
-
Hall DR, Gourley DG, Leonard GA, Duke EM, Anderson LA, Boxer DH, Hunter WN. 1999. The high-resolution crystal structure of the molybdate-dependent transcriptional regulator (ModE) from Escherichia coli: a novel combination of domain folds. EMBO J. 18:1435-1446.
-
(1999)
EMBO J.
, vol.18
, pp. 1435-1446
-
-
Hall, D.R.1
Gourley, D.G.2
Leonard, G.A.3
Duke, E.M.4
Anderson, L.A.5
Boxer, D.H.6
Hunter, W.N.7
-
4
-
-
0037459066
-
Crystal structure of activated ModE reveals conformational changes involving both oxyanion and DNA-binding domains
-
Schüttelkopf AW, Boxer DH, Hunter WN. 2003. Crystal structure of activated ModE reveals conformational changes involving both oxyanion and DNA-binding domains. J. Mol. Biol. 326:761-767.
-
(2003)
J. Mol. Biol.
, vol.326
, pp. 761-767
-
-
Schüttelkopf, A.W.1
Boxer, D.H.2
Hunter, W.N.3
-
5
-
-
0030614756
-
Characterization of the ModE DNA-binding sites in the control regions of modABCD and moaABCDE of Escherichia coli
-
McNicholas PM, Rech SA, Gunsalus RP. 1997. Characterization of the ModE DNA-binding sites in the control regions of modABCD and moaABCDE of Escherichia coli. Mol. Microbiol. 23:515-524.
-
(1997)
Mol. Microbiol.
, vol.23
, pp. 515-524
-
-
McNicholas, P.M.1
Rech, S.A.2
Gunsalus, R.P.3
-
6
-
-
0036267328
-
The molybdate-responsive Escherichia coli ModE transcriptional regulator coordinates periplasmic nitrate reductase (napFDAGHBC) operon expression with nitrate and molybdate availability
-
McNicholas PM, Gunsalus RP. 2002. The molybdate-responsive Escherichia coli ModE transcriptional regulator coordinates periplasmic nitrate reductase (napFDAGHBC) operon expression with nitrate and molybdate availability. J. Bacteriol. 184:3253-3259.
-
(2002)
J. Bacteriol.
, vol.184
, pp. 3253-3259
-
-
McNicholas, P.M.1
Gunsalus, R.P.2
-
7
-
-
0031962823
-
Anaerobic regulation of the Escherichia coli dmsABC operon requires the molybdate-responsive regulator ModE
-
McNicholas PM, Chiang RC, Gunsalus RP. 1998. Anaerobic regulation of the Escherichia coli dmsABC operon requires the molybdate-responsive regulator ModE. Mol. Microbiol. 27:197-208.
-
(1998)
Mol. Microbiol.
, vol.27
, pp. 197-208
-
-
McNicholas, P.M.1
Chiang, R.C.2
Gunsalus, R.P.3
-
8
-
-
70349100091
-
Participation of regulator AscG of the beta-glucoside utilization operon in regulation of the propionate catabolism operon
-
Ishida Y, Kori A, Ishihama A. 2009. Participation of regulator AscG of the beta-glucoside utilization operon in regulation of the propionate catabolism operon. J. Bacteriol. 191:6136-6144.
-
(2009)
J. Bacteriol.
, vol.191
, pp. 6136-6144
-
-
Ishida, Y.1
Kori, A.2
Ishihama, A.3
-
9
-
-
57349103117
-
The transcription regulator AllR senses both allantoin and glyoxylate and controls a set of genes for degradation and reutilization of purines
-
Hasegawa A, Ogasawara H, Kori A, Teramoto J, Ishihama A. 2008. The transcription regulator AllR senses both allantoin and glyoxylate and controls a set of genes for degradation and reutilization of purines. Microbiology 154:3366-3378.
-
(2008)
Microbiology
, vol.154
, pp. 3366-3378
-
-
Hasegawa, A.1
Ogasawara, H.2
Kori, A.3
Teramoto, J.4
Ishihama, A.5
-
10
-
-
56749183303
-
Anaerobic regulation of citrate fermentation by CitAB in Escherichia coli
-
Yamamoto K, Matsumoto F, Oshima T, Fujita N, Ogasawara N, Ishihama A. 2008. Anaerobic regulation of citrate fermentation by CitAB in Escherichia coli. Biosci. Biotechnol. Biochem. 72:3011-3014.
-
(2008)
Biosci. Biotechnol. Biochem.
, vol.72
, pp. 3011-3014
-
-
Yamamoto, K.1
Matsumoto, F.2
Oshima, T.3
Fujita, N.4
Ogasawara, N.5
Ishihama, A.6
-
11
-
-
24344490227
-
Systematic search for the Cra-binding promoters using genomic SELEX system
-
Shimada T, Fujita N, Maeda M, Ishihama A. 2005. Systematic search for the Cra-binding promoters using genomic SELEX system. Genes Cells 10:907-918.
-
(2005)
Genes Cells
, vol.10
, pp. 907-918
-
-
Shimada, T.1
Fujita, N.2
Maeda, M.3
Ishihama, A.4
-
12
-
-
78751473491
-
Novel members of the Cra regulon involved in carbon metabolism in Escherichia coli
-
Shimada T, Yamamoto K, Ishihama A. 2011. Novel members of the Cra regulon involved in carbon metabolism in Escherichia coli. J. Bacteriol. 193:649-659.
-
(2011)
J. Bacteriol.
, vol.193
, pp. 649-659
-
-
Shimada, T.1
Yamamoto, K.2
Ishihama, A.3
-
13
-
-
79957788648
-
Novel roles of cAMP receptor protein (CRP) in regulation of transport and metabolism of carbon sources
-
doi:10.1371/journal.pone .0020081
-
Shimada T, Fujita N, Yamamoto K, Ishihama A. 2011. Novel roles of cAMP receptor protein (CRP) in regulation of transport and metabolism of carbon sources. PLoS One 6:e20081. doi:10.1371/journal.pone .0020081.
-
(2011)
PLoS One
, vol.6
-
-
Shimada, T.1
Fujita, N.2
Yamamoto, K.3
Ishihama, A.4
-
14
-
-
77954358334
-
A novel nucleoid protein of Escherichia coli induced under anaerobiotic growth conditions
-
Teramoto J, Yoshimura SH, Takeyasu K, Ishihama A. 2010. A novel nucleoid protein of Escherichia coli induced under anaerobiotic growth conditions. Nucleic Acids Res. 38:3605-3618.
-
(2010)
Nucleic Acids Res.
, vol.38
, pp. 3605-3618
-
-
Teramoto, J.1
Yoshimura, S.H.2
Takeyasu, K.3
Ishihama, A.4
-
15
-
-
67650293863
-
Involvement of the leucine response transcription factor LeuO in regulation of the genes for sulfa drug efflux
-
Shimada T, Yamamoto K, Ishihama A. 2009. Involvement of the leucine response transcription factor LeuO in regulation of the genes for sulfa drug efflux. J. Bacteriol. 191:4562-4571.
-
(2009)
J. Bacteriol.
, vol.191
, pp. 4562-4571
-
-
Shimada, T.1
Yamamoto, K.2
Ishihama, A.3
-
16
-
-
50249134327
-
The uncharacterized transcription factor YdhM is the regulator of the nemA gene, encoding N-ethylmaleimide reductase
-
Umezawa Y, Shimada T, Kori A, Yamada K, Ishihama A. 2008. The uncharacterized transcription factor YdhM is the regulator of the nemA gene, encoding N-ethylmaleimide reductase. J. Bacteriol. 190:5890-5897.
-
(2008)
J. Bacteriol.
, vol.190
, pp. 5890-5897
-
-
Umezawa, Y.1
Shimada, T.2
Kori, A.3
Yamada, K.4
Ishihama, A.5
-
17
-
-
34547633841
-
PdhR (pyruvate dehydrogenase complex regulator) controls the respiratory electron transport system in Escherichia coli
-
Ogasawara H, Ishida Y, Yamada K, Yamamoto K, Ishihama A. 2007. PdhR (pyruvate dehydrogenase complex regulator) controls the respiratory electron transport system in Escherichia coli. J. Bacteriol. 189:5534-5541.
-
(2007)
J. Bacteriol.
, vol.189
, pp. 5534-5541
-
-
Ogasawara, H.1
Ishida, Y.2
Yamada, K.3
Yamamoto, K.4
Ishihama, A.5
-
18
-
-
84880194448
-
A novel regulator RcdA of the csgD gene encoding the master regulator of biofilm formation in Escherichia coli
-
Shimada T, Katayama Y, Kawakita S, Ogasawara H, Nakano M, Yamamoto K, Ishihama A. 2012. A novel regulator RcdA of the csgD gene encoding the master regulator of biofilm formation in Escherichia coli. Microbiologyopen 1:381-394.
-
(2012)
Microbiologyopen
, vol.1
, pp. 381-394
-
-
Shimada, T.1
Katayama, Y.2
Kawakita, S.3
Ogasawara, H.4
Nakano, M.5
Yamamoto, K.6
Ishihama, A.7
-
19
-
-
84873056329
-
Novel regulator PgrR for switch control of peptidoglycan recycling in Escherichia coli
-
Shimada T, Yamazaki K, Ishihama A. 2013. Novel regulator PgrR for switch control of peptidoglycan recycling in Escherichia coli. Genes Cells 18:123-134.
-
(2013)
Genes Cells
, vol.18
, pp. 123-134
-
-
Shimada, T.1
Yamazaki, K.2
Ishihama, A.3
-
20
-
-
34347374095
-
Genomic SELEX search for target promoters under the control of the PhoQP-RstBA signal relay cascade
-
Ogasawara H, Hasegawa A, Kanda E, Miki T, Yamamoto K, Ishihama A. 2007. Genomic SELEX search for target promoters under the control of the PhoQP-RstBA signal relay cascade. J. Bacteriol. 189:4791-4799.
-
(2007)
J. Bacteriol.
, vol.189
, pp. 4791-4799
-
-
Ogasawara, H.1
Hasegawa, A.2
Kanda, E.3
Miki, T.4
Yamamoto, K.5
Ishihama, A.6
-
21
-
-
35448941579
-
RutR is the uracil/thymine-sensing master regulator of a set of genes for synthesis and degradation of pyrimidines
-
Shimada T, Hirao K, Kori A, Yamamoto K, Ishihama A. 2007. RutR is the uracil/thymine-sensing master regulator of a set of genes for synthesis and degradation of pyrimidines. Mol. Microbiol. 66:744-757.
-
(2007)
Mol. Microbiol.
, vol.66
, pp. 744-757
-
-
Shimada, T.1
Hirao, K.2
Kori, A.3
Yamamoto, K.4
Ishihama, A.5
-
22
-
-
34547736490
-
folA, a new member of the TyrR regulon in Escherichia coli K-12
-
Yang J, Ogawa Y, Camakaris H, Shimada T, Ishihama A, Pittard AJ. 2007. folA, a new member of the TyrR regulon in Escherichia coli K-12. J. Bacteriol. 189:6080-6084.
-
(2007)
J. Bacteriol.
, vol.189
, pp. 6080-6084
-
-
Yang, J.1
Ogawa, Y.2
Camakaris, H.3
Shimada, T.4
Ishihama, A.5
Pittard, A.J.6
-
23
-
-
31544450286
-
Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection
-
2006.0008
-
Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H. 2006. Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2:2006.0008.
-
(2006)
Mol. Syst. Biol.
, vol.2
-
-
Baba, T.1
Ara, T.2
Hasegawa, M.3
Takai, Y.4
Okumura, Y.5
Baba, M.6
Datsenko, K.A.7
Tomita, M.8
Wanner, B.L.9
Mori, H.10
-
24
-
-
0031028408
-
Variation in RNA polymerase sigma subunit composition within different stocks of Escherichia coli strain W3110
-
Jishage M, Ishihama A. 1997. Variation in RNA polymerase sigma subunit composition within different stocks of Escherichia coli strain W3110. J. Bacteriol. 179:959-963.
-
(1997)
J. Bacteriol.
, vol.179
, pp. 959-963
-
-
Jishage, M.1
Ishihama, A.2
-
25
-
-
12544258487
-
Functional characterization in vitro of all two-component signal transduction systems from Escherichia coli
-
Yamamoto K, Hirao K, Oshima T, Aiba H, Utsumi R, Ishihama A. 2005. Functional characterization in vitro of all two-component signal transduction systems from Escherichia coli. J. Biol. Chem. 280:1448-1456.
-
(2005)
J. Biol. Chem.
, vol.280
, pp. 1448-1456
-
-
Yamamoto, K.1
Hirao, K.2
Oshima, T.3
Aiba, H.4
Utsumi, R.5
Ishihama, A.6
-
26
-
-
0023255472
-
Improved single and multicopy lac-based cloning vectors for protein and operon fusions
-
Simons RW, Houman F, Kleckner N. 1987. Improved single and multicopy lac-based cloning vectors for protein and operon fusions. Gene 53: 85-96.
-
(1987)
Gene
, vol.53
, pp. 85-96
-
-
Simons, R.W.1
Houman, F.2
Kleckner, N.3
-
27
-
-
0003785155
-
-
Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY
-
Miller JH. 1972. Experiments in molecular genetics. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
-
(1972)
Experiments in molecular genetics
-
-
Miller, J.H.1
-
28
-
-
24944583149
-
Transcriptional response of Escherichia coli to external zinc
-
Yamamoto K, Ishihama A. 2005. Transcriptional response of Escherichia coli to external zinc. J. Bacteriol. 187:6333-6340.
-
(2005)
J. Bacteriol.
, vol.187
, pp. 6333-6340
-
-
Yamamoto, K.1
Ishihama, A.2
-
29
-
-
16244398023
-
Transcriptional response of Escherichia coli to external copper
-
Yamamoto K, Ishihama A. 2005. Transcriptional response of Escherichia coli to external copper. Mol. Microbiol. 56:215-227.
-
(2005)
Mol. Microbiol.
, vol.56
, pp. 215-227
-
-
Yamamoto, K.1
Ishihama, A.2
-
30
-
-
79956134862
-
Role of the biofilm master regulator CsgD in cross-regulation between biofilm formation and flagellar synthesis
-
Ogasawara H, Yamamoto K, Ishihama A. 2011. Role of the biofilm master regulator CsgD in cross-regulation between biofilm formation and flagellar synthesis. J. Bacteriol. 193:2587-2597.
-
(2011)
J. Bacteriol.
, vol.193
, pp. 2587-2597
-
-
Ogasawara, H.1
Yamamoto, K.2
Ishihama, A.3
-
31
-
-
80052781624
-
Induction of the Escherichia coli cysK gene by genetic and environmental factors
-
Yamamoto K, Oshima T, Nonaka G, Ito H, Ishihama A. 2011. Induction of the Escherichia coli cysK gene by genetic and environmental factors. FEMS Microbiol. Lett. 323:88-95.
-
(2011)
FEMS Microbiol. Lett.
, vol.323
, pp. 88-95
-
-
Yamamoto, K.1
Oshima, T.2
Nonaka, G.3
Ito, H.4
Ishihama, A.5
-
32
-
-
0016231892
-
Isolation of beta-galactosidase by chromatography
-
Steers E, Jr, Cuatrecasas P. 1974. Isolation of beta-galactosidase by chromatography. Methods Enzymol. 34:350-358.
-
(1974)
Methods Enzymol.
, vol.34
, pp. 350-358
-
-
Steers Jr., E.1
Cuatrecasas, P.2
-
34
-
-
42649103655
-
W-AlignACE: an improved Gibbs sampling algorithm based on more accurate position weight matrices learned from sequence and gene expression/ChIP-chip data
-
Chen X, Guo L, Fan Z, Jiang T. 2008. W-AlignACE: an improved Gibbs sampling algorithm based on more accurate position weight matrices learned from sequence and gene expression/ChIP-chip data. Bioinformatics 24:1121-1128.
-
(2008)
Bioinformatics
, vol.24
, pp. 1121-1128
-
-
Chen, X.1
Guo, L.2
Fan, Z.3
Jiang, T.4
-
35
-
-
79952382558
-
The Escherichia coli K-12 MntR miniregulon includes dps which encodes the major stationary-phase DNA-binding protein
-
Yamamoto K, Ishihama A, Busby SJW, Grainger DC. 2011. The Escherichia coli K-12 MntR miniregulon includes dps which encodes the major stationary-phase DNA-binding protein. J. Bacteriol. 193:1477-1480.
-
(2011)
J. Bacteriol.
, vol.193
, pp. 1477-1480
-
-
Yamamoto, K.1
Ishihama, A.2
Busby, S.J.W.3
Grainger, D.C.4
-
36
-
-
0023655931
-
Sequence elements essential for rhodependent transcription termination at lambda tR1
-
Chen CY, Richardson JP. 1987. Sequence elements essential for rhodependent transcription termination at lambda tR1. J. Biol. Chem. 262: 11292-11299.
-
(1987)
J. Biol. Chem.
, vol.262
, pp. 11292-11299
-
-
Chen, C.Y.1
Richardson, J.P.2
-
37
-
-
0141651638
-
The Escherichia coli ynfEFGHI operon encodes polypeptides which are paralogues of dimethyl sulfoxide reductase (DmsABC)
-
Lubitz SP, Weiner JH. 2003. The Escherichia coli ynfEFGHI operon encodes polypeptides which are paralogues of dimethyl sulfoxide reductase (DmsABC). Arch. Biochem. Biophys. 418:205-216.
-
(2003)
Arch. Biochem. Biophys.
, vol.418
, pp. 205-216
-
-
Lubitz, S.P.1
Weiner, J.H.2
-
38
-
-
27744479572
-
YvcK of Bacillus subtilis is required for a normal cell shape and for growth on Krebs cycle intermediates and substrates of the pentose phosphate pathway
-
Görke B, Foulquier E, Galinier A. 2005. YvcK of Bacillus subtilis is required for a normal cell shape and for growth on Krebs cycle intermediates and substrates of the pentose phosphate pathway. Microbiology 151:3777-3791.
-
(2005)
Microbiology
, vol.151
, pp. 3777-3791
-
-
Görke, B.1
Foulquier, E.2
Galinier, A.3
-
40
-
-
65549127857
-
Activation and repression at the Escherichia coli ynfEFGHI operon promoter
-
Xu M, Busby SJ, Browning DF. 2009. Activation and repression at the Escherichia coli ynfEFGHI operon promoter. J. Bacteriol. 191:3172-3176.
-
(2009)
J. Bacteriol.
, vol.191
, pp. 3172-3176
-
-
Xu, M.1
Busby, S.J.2
Browning, D.F.3
-
41
-
-
27744521471
-
Global gene expression analysis revealed an unsuspected deo operon under the control of molybdate sensor, ModE protein, in Escherichia coli
-
Tao H, Hasona A, Do PM, Ingram LO, Shanmugam KT. 2005. Global gene expression analysis revealed an unsuspected deo operon under the control of molybdate sensor, ModE protein, in Escherichia coli. Arch. Microbiol. 184:225-233.
-
(2005)
Arch. Microbiol.
, vol.184
, pp. 225-233
-
-
Tao, H.1
Hasona, A.2
Do, P.M.3
Ingram, L.O.4
Shanmugam, K.T.5
-
42
-
-
0033061571
-
Initiation of translation in prokaryotes and eukaryotes
-
Kozak M. 1999. Initiation of translation in prokaryotes and eukaryotes. Gene 234:187-208.
-
(1999)
Gene
, vol.234
, pp. 187-208
-
-
Kozak, M.1
-
43
-
-
15444350252
-
The complete genome sequence of Escherichia coli K-12
-
Blattner FR, Plunkett G, III, Bloch CA, Perna NT, Burland V, Riley M, Collado-Vides J, Glasner JD, Rode CK, Mayhew GF, Gregor J, Davis NW, Kirkpatrick HA, Goeden MA, Rose DJ, Mau B, Shao Y. 1997. The complete genome sequence of Escherichia coli K-12. Science 277:1453-1462.
-
(1997)
Science
, vol.277
, pp. 1453-1462
-
-
Blattner, F.R.1
Plunkett III, G.2
Bloch, C.A.3
Perna, N.T.4
Burland, V.5
Riley, M.6
Collado-Vides, J.7
Glasner, J.D.8
Rode, C.K.9
Mayhew, G.F.10
Gregor, J.11
Davis, N.W.12
Kirkpatrick, H.A.13
Goeden, M.A.14
Rose, D.J.15
Mau, B.16
Shao, Y.17
-
44
-
-
0028904488
-
AUC is used as a start codon in Escherichia coli
-
Chalut C, Eglay JM. 1995. AUC is used as a start codon in Escherichia coli. Gene 156:43-45.
-
(1995)
Gene
, vol.156
, pp. 43-45
-
-
Chalut, C.1
Eglay, J.M.2
-
45
-
-
0025954907
-
Initiation of translation at an AUA codon for an archaebacterial protein gene expressed in E
-
Köpke AK, Leggatt PA. 1991. Initiation of translation at an AUA codon for an archaebacterial protein gene expressed in E. coli. Nucleic Acids Res. 19:5169-5172.
-
(1991)
coli. Nucleic Acids Res.
, vol.19
, pp. 5169-5172
-
-
Köpke, A.K.1
Leggatt, P.A.2
-
46
-
-
0026289515
-
Initiation of translation at AUC, AUA and AUU codons in Escherichia coli
-
Romero A, García P. 1991. Initiation of translation at AUC, AUA and AUU codons in Escherichia coli. FEMS Microbiol. Lett. 68:325-330.
-
(1991)
FEMS Microbiol. Lett.
, vol.68
, pp. 325-330
-
-
Romero, A.1
García, P.2
-
47
-
-
0025280234
-
Initiation of in vivo protein synthesis with non-methionine amino acids
-
Chattapadhyay R, Pelka H, Schulman LH. 1990. Initiation of in vivo protein synthesis with non-methionine amino acids. Biochemistry 29: 4263-4268.
-
(1990)
Biochemistry
, vol.29
, pp. 4263-4268
-
-
Chattapadhyay, R.1
Pelka, H.2
Schulman, L.H.3
-
48
-
-
0032795785
-
Discrimination by Escherichia coli initiation factor IF3 against initiation on non-canonical codons relies on complementarity rules
-
Meinnel T, Sacerdot C, Graffe M, Blanquet S, Springer M. 1999. Discrimination by Escherichia coli initiation factor IF3 against initiation on non-canonical codons relies on complementarity rules. J. Mol. Biol. 290:825-837.
-
(1999)
J. Mol. Biol.
, vol.290
, pp. 825-837
-
-
Meinnel, T.1
Sacerdot, C.2
Graffe, M.3
Blanquet, S.4
Springer, M.5
-
49
-
-
60149089144
-
Regulatory RNAs in bacteria
-
Waters LS, Storz G. 2009. Regulatory RNAs in bacteria. Cell 136:615-628.
-
(2009)
Cell
, vol.136
, pp. 615-628
-
-
Waters, L.S.1
Storz, G.2
|