Method for the detection of priority shiga toxin-producing Escherichia coli in beef trim

Journal of Food Protection

Volumn 76, Issue 10, 2013, Pages 1689-1696

  Huszczynski, George ^a   Gauthier, Martine ^a   Mohajer, Sam ^a   Gill, Alexander ^b   Blais, Burton ^a  

^a CANADIAN FOOD INSPECTION AGENCY   (Canada)

^b HEALTH CANADA   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

AGAR; VIRULENCE FACTOR;

ANIMAL; ARTICLE; BACTERIAL COUNT; CATTLE; CLASSIFICATION; FOOD CONTAMINATION; FOOD CONTROL; GENETICS; ISOLATION AND PURIFICATION; MEAT; METHODOLOGY; MICROBIOLOGY; POLYMERASE CHAIN REACTION; SEROTYPING; SHIGA TOXIN PRODUCING ESCHERICHIA COLI;

AGAR; ANIMALS; CATTLE; COLONY COUNT, MICROBIAL; FOOD CONTAMINATION; FOOD MICROBIOLOGY; MEAT; POLYMERASE CHAIN REACTION; SEROTYPING; SHIGA-TOXIGENIC ESCHERICHIA COLI; VIRULENCE FACTORS;

EID: 84885440135     PISSN: 0362028X     EISSN: None     Source Type: Journal    
DOI: 10.4315/0362-028X.JFP-13-059     Document Type: Article

References (17)

1. Anonymous. March 2011. Guidelines for the relative validation of indirect qualitative food microbiological methods (part 4). Compendium of analytical methods, vol. 1, Official methods for the microbiological analysis of foods. Available at: http://www.hc-sc.gc.ca/fn-an/res-rech/analy-meth/microbio/volume1/ index-eng.php. Accessed 25 January 2013.
2. Anonymous. November 2012. Detection and isolation of non-O157 Shiga toxin-producing Escherichia coli (STEC) from meat products (method 5B.03). Guidebooks and methods - United States Department of Agriculture Food Safety and Inspection Service. Available at: http://www.fsis.usda.gov/science/ microbiological-guidebook. Accessed 25 January 25 2013.
3. Blais, B. W., M. Gauthier, M. Deschênes, and G. Huszczynski. 2012. Polyester cloth-based hybridization array system for identification of enterohemorrhagic Escherichia coli serogroups O26, O45, O103, O111, O121, O145 and O157. J. Food Prot. 75: 1691-1697.
4. Blais, B. W., and A. Martinez-Perez. 2011. A simple PCR-based macroarray system for detection of multiple gene markers in the identification of priority enterohemorrhagic Escherichia coli. J. Food Prot. 74: 365-372.
5. Brooks J., E. Sowers, J. Wells, K. Greene, P. Griffin, R. Hoekstra, and N. Strockbine. 2005. Non-O157 Shiga toxin-producing Escherichia coli infections in the United States, 1983-2002. J. Infect. Dis. 192: 1422-1429.
6. Gill, A., A. Martinez-Perez, S. McIlwham, and B. Blais. 2012. Development of a method for the detection of verotoxin producing Escherichia coli in food. J. Food Prot. 75: 827-837.
7. Gilmour, M. W., A. B. Olson, A. K. Andrysiak, L.-K. Ng, and L. Chui. 2007. Sequence-based typing of genetic targets encoded outside of the O-antigen gene cluster is indicative of Shiga toxinproducing Escherichia coli serogroup lineages. J. Med. Microbiol. 56: 620-628.
8. Kanki, M., K. Seto, T. Harada, S. Yonogi, and Y. Kumeda. 2011. Comparison of four enrichment broths for the detection of non-O157 Shiga-toxin-producing Escherichia coli O91, O103, O111, O119, O121, O145 and O165 from pure culture and food samples. Lett. Appl. Microbiol. 53: 167-173.
9. Kaper, J., J. Nataro, and H. Mobley. 2004. Pathogenic Escherichia coli. Nat. Rev. Microbiol. 2: 123-140.
10. Karmali, M. A., B. T. Steele, M. Petric, and C. Lim. 1983. Sporadic cases of haemolytic-uraemic syndrome associated with faecal cytotoxin and cytotoxin-producing Escherichia coli in stools. Lancet 1: 619-620.
11. Leggate, J., and B. W. Blais. 2006. An internal amplification control system based on primer-dimer formation for PCR product detection by DNA hybridization. J. Food Prot. 69: 2280-2284.
12. Mathusa, E., Y. Chen, E. Enache, and L. Hontz. 2010. Non-O157 Shiga toxin-producing Escherichia coli in foods. J. Food Prot. 73: 1721-1736.
13. Melton-Celsa, A., K. Mohawk, L. Teel, and A. O'Brien. 2012. Pathogenesis of Shiga-toxin producing Escherichia coli. Curr. Top. Microbiol. Immunol. 357: 67-103.
14. Rangel, J. M., P. H. Sparling, C. Crowe, P. M. Griffin, and D. L. Swerdlow. 2005. Epidemiology of Escherichia coli O157:H7 outbreaks, United States, 1982-2002. Emerg. Infect. Dis. 11: 603-609.
15. Scallan, E., R. M. Hoekstra, F. J. Angulo, R. V. Tauxe, M.-A. Widdowson, S. L. Roy, J. L. Jones, and P. M. Griffin. 2011. Foodborne illness acquired in the United States - major pathogens. Emerg. Infect. Dis. 17: 7-15.
16. Tarr, P. I., C. A. Gordon, and W. L. Chandler. 2005. Shiga-toxinproducing Escherichia coli and haemolytic uraemic syndrome. Lancet 365: 1073-1086.
17. Vimont, A., M.-L. Delignette-Muller, and C. Vernozy-Rozand. 2007. Supplementation of enrichment broths by novobiocin for detecting Shiga toxin-producing Escherichia coli from food: a controversial use. Lett. Appl. Microbiol. 44: 326-331.