Quantitative turbidity assay for lipolytic enzymes in microtiter plates

Analytical and Bioanalytical Chemistry

Volumn 405, Issue 26, 2013, Pages 8539-8547

  Barig, Susann ^a   Schiemann, Manja ^a   Mirsky, Vladimir M ^a   Stahmann, K Peter ^a  

^a BRANDENBURG UNIVERSITY OF TECHNOLOGY   (Germany)

Author keywords

Lipase assay; Lipolytic enzymes; Microtiter plates; Tributyrin; Turbidity

Indexed keywords

96-WELL MICROTITER PLATE; ENZYME CONCENTRATIONS; LIPOLYTIC ENZYMES; MICROTITER PLATE READER; MICROTITER PLATES; POLYACRYLAMIDE GELS; PORCINE PANCREATIC LIPASE; TRIBUTYRIN;

ALGAE; DIFFUSION; EMULSIFICATION; ETHYLENE; GELS; POLYSACCHARIDES; PROPYLENE; SIGNAL DETECTION; TURBIDITY;

LIPASES;

SUS;

TRIACYLGLYCEROL; TRIACYLGLYCEROL LIPASE; TRIBUTYRIN;

ANIMAL; ARTICLE; BACTERIUM; CHEMISTRY; EMULSION; ENZYME ASSAY; ENZYME SPECIFICITY; ENZYMOLOGY; EVALUATION STUDY; FUNGUS; KINETICS; LIMIT OF DETECTION; METABOLISM; METHODOLOGY; SWINE;

ANIMALS; BACTERIA; EMULSIONS; ENZYME ASSAYS; FUNGI; KINETICS; LIMIT OF DETECTION; LIPASE; SUBSTRATE SPECIFICITY; SWINE; TRIGLYCERIDES;

EID: 84885435224     PISSN: 16182642     EISSN: 16182650     Source Type: Journal    
DOI: 10.1007/s00216-013-7283-5     Document Type: Article

References (50)

1. Sharma R, Chisti Y, Banerjee UC (2001) Production, purification, characterization, and application of lipases. Biotechnol Adv 19:627-662
2. Koizumi M, Takada T, Kawarada Y, Hirata K, Mayumi T, Yoshida M, Sekimoto M, Hirota M, Kimura Y, Takeda K, Isaji S, Otsuki M, Matsuno S (2006) JPN Guidelines for the management of acute pancreatitis: diagnostic criteria for acute pancreatitis. J Hepatobiliary Pancreat Surg 13:25-32
3. Matull WR, Pereira SP, O'Donohue JW (2006) Biochemical marker of acute pancreatitis. J Clin Pathol 59:340-344
4. Rollof J, Hedström SA, Nilsson-Ehle P (1984) A simple turbidimetric method for specific measurement of Staphylococcus aureus lipase activity. Acta Path Microbiol Immunol Scand Sect B 92:155-158
5. Smeltzer MS, Hart ME, Iandolo JJ (1992) Quantitative spectrophotometric assay for staphylococcal lipase. Appl Environ Microbiol 58:2815-2819
6. Jensen RG (1983) Detection and determination of lipase (acylglycerol hydrolase) activity from various sources. Lipids 18:650-657
7. Beisson F, Tiss A, Rivière C, Verger R (2000) Methods for lipase detection and assay: a critical review. Eur J Lipid Sci Technol 102:133-153
8. Gupta R, Rathi P, Gupta N, Bradoo S (2003) Lipase assays for conventional and molecular screening: an overview. Biotechnol Appl Biochem 37:63-71
9. Nury S, Piéroni G, Rivière C, Gargouri Y, Bois A, Verger R (1987) Lipase kinetics at the triacylglycerol-water interface using surface tension measurements. Chem Phys Lipids 45:27-37
10. Colacicco G, Rapport MM (1966) Lipid monolayers: action of phospholipase A of Crotalus atrox and Naja naja venoms on phosphatidyl choline and phosphatidal choline. J Lipid Res 7:258-263
11. 2 action towards lipid monolayer. Chem Phys Lipids 70:75-81
12. Mirsky VM, Krause C, Heckmann KD (1996) Capacitive sensor for lipolytic enzymes. Thin Solid Films 284(285):939-941
13. 2 activity towards artificial and natural substrates. Anal Chem 70:3674-3678
14. El Kirat K, Morandat S, Dufrêne YF (2010) Nanoscale analysis of supported lipid bilayers using atomic force microscopy. Biochim Biophys Acta 1798:750-765
15. Mirsky VM, Cherny VV, Sokolov VS, Markin VS (1990) Electrostatic assay of phospholipase A activity: an application of the second harmonic method of monitoring membrane boundary potentials. J Biochem Biophys Meth 21:277-284
16. 2 activity. Biol Mem 6:971-982 (translation of (1992) Biologicheskie Membrany 9:733-740)
17. Walde P, Luisi PL (1989) A continuous assay for lipase in reverse micelles based on fourier transform infrared spectroscopy. Biochem 28:3353-3360
18. Bronscheuer UT, Kazlauskas RJ (2006) Hydrolases in organic synthesis: regio- and stereoselective biotransformations. Wiley-VCH, Weinheim
19. Winkler UK, Stuckmann M (1979) Glycogen, hyaluronate, and some other polysaccharides greatly enhance the formation of exolipase by Serratia marcescens. J Bacteriol 138:663-670
20. Helistö P, Korpela T (1998) Effects of detergents on activity of microbial lipases as measured by the nitrophenyl alkanoate esters method. Enzyme Microb Technol 23:113-117
21. Schmidt-Dannert C, Sztajer H, Stöcklein W, Menge U, Schmid RD (1994) Screening, purification and properties of a thermophilic lipase from Bacillus thermocatenulatus. Biochim Biophys Acta 1214:43-53
22. Proelss HF, Wright BW (1977) Lipoxygenic micromethod for specific determination of lipase activity in serum and duodenal fluid. Clin Chem 23:522-531
23. Ulitzur S (1979) A sensitive bioassay for lipase using bacterial bioluminescence. Biochem Biophys Acta 572:211-217
24. Aoubala M, Ivanova M, Douchet I, De Caro A, Verger R (1995) Interfacial binding of human gastric lipase to lipid monolayers, measured with an ELISA. Biochem 34:10786-10793
25. Lawrence RC, Fryer TF, Reiter B (1967) Rapid method for the quantitative estimation of microbial lipases. Nature 213:1264-1265
26. Lima N, Teixeira JA, Mota M (1991) Deep agar-diffusion test for preliminary screening of lipolytic activity of fungi. J Microbiol Methods 14:193-200
27. Samad MYA, Razak CNA, Bakar Salleh A, Wan Yunus WMZ, Ampon K, Basri M (1989) A plate assay for primary screening of lipase activity. J Microbiol Methods 9:51-56
28. Owusu RK, Makhzoum A, Knapp JS (1992) Heat inactivation of lipase from psychrotrophic Pseudomonas fluorescens P38: activation parameters and enzymes stability at low or ultra-high temperatures. Food Chem 44:261-268
29. Delorme V, Dhouib R, Canaan S, Fotiadu F, Carrière F, Cavalier JF (2011) Effects of surfactants on lipase structure, activity, and inhibition. Pharm Res 28:1831-1842
30. Aloulou A, Puccinelli D, Caro AD, Leblond Y, Carrière F (2007) A comparative study on two fungal lipases from Thermomyces lanuginosus and Yarrowia lioplytica shows the combined effects of detergents and pH on lipase adsorption and activity. Biochim Biophys Acta 1771:1446-1456
31. Sonesson AW, Elofsson UM, Brismar H, Callisen TH (2006) Adsorption and mobility of a lipase at a hydrophobic surface in the presence of surfactants. Langmuir 22:5810-5817
32. Morgensen JE, Sehgal P, Otzen DE (2005) Activation, inhibition, and destabilization of Thermomyces lanuginosus lipase by detergents. Biochem 44:1719-1730
33. Jha BK, Svensson M, Kronberg B, Holmberg K (1999) Titration microcalorimetry studies of the interaction between Humicola lanuginosa lipase and ionic surfactants. J Coll Interf Sci 213:262-264
34. Barig S, Alisch R, Nieland S, Wuttke A, Gräser Y, Huddar M, Schnitzlein K, Stahmann KP (2011) Monoseptic growth of fungal lipase producers under minimized sterile conditions: cultivation of Phialemonium curvatum in 350 L scale. Eng Life Sci 11:387-394
35. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248-254
36. Grossmann K, Roggenbuck D, Schröder C, Conrad K, Schierack P, Sack U (2011) Multiplex assessment of non-organ-specific autoantibodies with novel microbead-based immunoassay. Cytometry A 79A:118-125
37. Pinholt C, Fano M, Wiberg C, Hostrup S, Thostrup Bukrinsky J, Frokjaer S, Norde W, Jorgensen L (2010) Influence of glycosylation on the adsorption of Thermomyces lanuginosus lipase to hydrophobic and hydrophilic surfaces. Eur J Pharm Sci 40:273-281
38. Sugiura M, Isobe M (1975) Studies on the mechanism of the lipase reaction II. Comparative studies on the adsorption of lipase and various proteins at the air-water interface. Biochim Biophys Acta 397:412-417
39. Brockerhoff H (1974) In: Whitaker JR (ed) Food related enzymes. American Chemical Society, Washington, DC
40. Ali YB, Verger R, Abousalham A (2012) In: Sandoval G (ed) Lipases and phospholipases: methods and protocols. Springer, New York
41. Jurado E, Camacho F, Luzón G, Fernández-Serrano M, García-Román M (2006) Kinetic model for the enzymatic hydrolysis of tributyrin in o/w emulsions. Chem Eng Sci 61:5010-5020
42. Duckworth M, Yaphe W (1971) The structure of agar: part 1. Fractionation of a complex mixture of polysaccharides. Carbohydr Res 16:189-197
43. Savart T, Dove C, Love BJ (2010) In situ dynamic rheological study of polyacrylamide during gelation coupled with mathematical models of viscosity advancement. Macromol Mater Eng 295:146-152
44. 2 and regulation of interfacial activation: hopping versus scooting. Biochim Biophys Acta 1002:127-156
45. Jurado E, Camacho F, Luzón G, Fernández-Serrano M, García-Román M (2008) Kinetics of the enzymatic hydrolysis of triglycerides in o/w emulsions, study of the initial rates and the reaction time course. Biochem Eng J 40:473-484
46. Lifshitz IM, Slyozov VV (1961) The kinetics of precipitation from supersaturated solid solutions. J Phys Chem Solids 19:35-50
47. Murphy CA, Cameron JA, Huang SJ, Vinopal RT (1998) A second polycaprolactone depolymerase from Fusarium, a lipase distinct from cutinase. Appl Microbiol Biotechnol 50:692-696
48. Li Y, Le Maux S, Xiao H, McClements DJ (2009) Emulsion-based delivery systems for tributyrin, a potential colon cancer preventative agent. J Agric Food Chem 57:9243-9249
49. Kwan SF, Hunt RJ (1995) Lipase single reagent system. U.S. Patent 5,378,609
50. Fernandez-Lafuente R (2010) Lipase from Thermomyces lanuginosus: uses and prospects as an industrial biocatalyst. J Mol Catal B Enzym 62:197-212