Rapid Mutation of Spirulina platensis by a New Mutagenesis System of Atmospheric and Room Temperature Plasmas (ARTP) and Generation of a Mutant Library with Diverse Phenotypes

PLoS ONE

Volumn 8, Issue 10, 2013, Pages

  Fang, Mingyue ^a   Jin, Lihua ^a,b   Zhang, Chong ^a   Tan, Yinyee ^a   Jiang, Peixia ^a   Ge, Nan ^a   Li, Heping ^a   Xing, Xinhui ^a  

^a TSINGHUA UNIVERSITY   (China)

^b Beijing Polytechnic   (China)

Author keywords

[No Author keywords available]

Indexed keywords

CARBOHYDRATE; HELIUM;

AIR TEMPERATURE; ARTICLE; ATMOSPHERIC AND ROOM TEMPERATURE PLASMA MUTATION SYSTEM; BACTERIAL GROWTH; BACTERIAL MUTATION; BACTERIAL STRAIN; BACTERIUM CULTURE; CARBOHYDRATE ANALYSIS; CARBOHYDRATE SYNTHESIS; CONTROLLED STUDY; FLOCCULATION; GENETIC STABILITY; GENETIC VARIABILITY; GROWTH RATE; HIGH THROUGHPUT SCREENING; MEDICAL DEVICE; MICROPLATE READER; MOLECULAR LIBRARY; MUTAGENESIS; MUTATIONAL ANALYSIS; NONHUMAN; PHENOTYPE; ROOM TEMPERATURE; SPIRULINA PLATENSIS; STRAIN DIFFERENCE;

GENE LIBRARY; GENETIC ENGINEERING; GENOME, BACTERIAL; GENOMIC INSTABILITY; HELIUM; MUTAGENESIS; MUTAGENS; PHENOTYPE; PLASMA GASES; SPIRULINA;

EID: 84885412313     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0077046     Document Type: Article

References (48)

1. Markou G, Georgakakis D, (2011) Cultivation of filamentous cyanobacteria (blue-green algae) in agro-industrial wastes and wastewaters: A review. Appl Energ 88: 3389-3401. doi:10.1016/j.apenergy.2010.12.042.
2. Ortega-Calvo J, Mazuelos C, Hermosin B, Saiz-Jimenez C, (1993) Chemical composition of Spirulina and eukaryotic algae food products marketed in Spain. J Appl Phycol 5: 425-435. doi:10.1007/BF02182735.
3. Harun R, Singh M, Forde GM, Danquah MK, (2010) Bioprocess engineering of microalgae to produce a variety of consumer products. Renewable Sustain Energ Rev 14: 1037-1047. doi:10.1016/j.rser.2009.11.004.
4. Pangestuti R, Kim S-K, (2011) Biological activities and health benefit effects of natural pigments derived from marine algae. J Funct Foods 3: 255-266. doi:10.1016/j.jff.2011.07.001.
5. Vasconcelos VM, Martins J, Peixe L, (2011) Unraveling Cyanobacteria Ecology in Wastewater Treatment Plants (WWTP). Microb Ecol 62: 241-256. doi:10.3354/ame01461. PubMed: 21287346.
6. Logan C, Sims R, (2011) Production and harvesting of microalgae for wastewater treatment, biofuels, and bioproducts. Biotechnol Adv 29: 686-702. doi:10.1016/j.biotechadv.2011.05.015. PubMed: 21664266.
7. Pittman JK, Dean AP, Osundeko O, (2011) The potential of sustainable algal biofuel production using wastewater resources. Bioresour Technol 102: 17-25. doi:10.1016/j.biortech.2010.06.035. PubMed: 20594826.
8. Chinnasamy S, Bhatnagar A, Hunt RW, Das KC, (2010) Microalgae cultivation in a wastewater dominated by carpet mill effluents for biofuel applications. Bioresour Technol 101: 3097-3105. doi:10.1016/j.biortech.2009.12.026. PubMed: 20053551.
9. Rawat I, Ranjith Kumar R, Mutanda T, Bux F, (2011) Dual role of microalgae: Phycoremediation of domestic wastewater and biomass production for sustainable biofuels production. Appl Energ 88: 3411-3424. doi:10.1016/j.apenergy.2010.11.025.
10. Bezerra RP, Montoya EYO, Sato S, Perego P, de Carvalho JCM, et al. (2011) Effects of light intensity and dilution rate on the semicontinuous cultivation of Arthrospira (Spirulina) platensis. A kinetic Monod-type approach. Bioresour Technol 102: 3215-3219. doi:10.1016/j.biortech.2010.11.009. PubMed: 21111613.
11. Hu Q, Sommerfeld M, Jarvis E, Ghirardi M, Posewitz M, et al. (2008) Microalgal triacylglycerols as feedstocks for biofuel production: perspectives and advances. Plant J 54: 621-639. doi:10.1111/j.1365-313X.2008.03492.x. PubMed: 18476868.
12. Brennan L, Owende P, (2010) Biofuels from microalgae-A review of technologies for production, processing, and extractions of biofuels and co-products. Renewable Sustain Energ Rev 14: 557-577. doi:10.1016/j.rser.2009.10.009.
13. Amaro HM, Guedes AC, Malcata FX, (2011) Advances and perspectives in using microalgae to produce biodiesel. Appl Energ 88: 3402-3410. doi:10.1016/j.apenergy.2010.12.014.
14. Xu L, Brilman DWF, Withag JAM, Brem G, Kersten S, (2011) Assessment of a dry and a wet route for the production of biofuels from microalgae: Energy balance analysis. Bioresour Technol 102: 5113-5122. doi:10.1016/j.biortech.2011.01.066. PubMed: 21334889.
15. Matsumoto M, Yokouchi H, Suzuki N, Ohata H, Matsunaga T, (2003) Saccharification of marine microalgae using marine bacteria for ethanol production. Appl Biochem Biotechnol 105: 247-254. doi:10.1385/ABAB:105:1-3:247. PubMed: 12721490.
16. Hirayama S, Ueda R, Ogushi Y, Hirano A, Samejima Y, et al. (1998) Ethanol production from carbon dioxide by fermentative microalgae. In: Yamaguchi T, eds., T Inui MAKISY. Studies in Surface Science and Catalysis: Elsevier. pp. pp. 657-660.
17. Harun R, Danquah MK, Forde GM, (2010) Microalgal biomass as a fermentation feedstock for bioethanol production. J Chem Technol Biotechnol 85: 199-203.
18. Mohapatra TM, Singh RK, Tiwari SP, Rai AK, (2011) Cyanobacteria: an emerging source for drug discovery. J Antibiot 64: 401-412. doi:10.1038/ja.2011.21. PubMed: 21468079.
19. Borowitzka MA, Borowitzka LJ, editors (1988) Micro-algal biotechnology. Cambridge: Cambridge University Press. 395 pp.
20. Ciferri O, (1983) Spirulina, the edible microorganism. Microbiol Rev 47: 551-578. PubMed: 6420655.
21. Aikawa S, Izumi Y, Matsuda F, Hasunuma T, Chang J-S, et al. (2012) Synergistic enhancement of glycogen production in Arthrospira platensis by optimization of light intensity and nitrate supply. Bioresour Technol 108: 211-215. doi:10.1016/j.biortech.2012.01.004. PubMed: 22277210.
22. Spolaore P, Joannis-Cassan C, Duran E, Isambert A, (2006) Commercial applications of microalgae. J Biosci Bioeng 101: 87-96. doi:10.1263/jbb.101.87. PubMed: 16569602.
23. Kawamura M, Sakakibara M, Watanabe T, Kita K, Hiraoka N, et al. (1986) A New Restriction Endonuclease from Spirulina-Platensis. Nucleic Acids Res 14: 1985-1989. doi:10.1093/nar/14.5.1985. PubMed: 3008081.
24. Vonshak A, (1997) Spirulina platensis, physiology, cell-biology and biotechnology. London: Taylor & Francis Ltd.
25. Toyomizu M, Suzuki K, Kawata Y, Kojima H, Akiba Y, (2001) Effective transformation of the cyanobacterium Spirulina platensis using electroporation. J Appl Phycol 13: 209-214. doi:10.1023/A:1011182613761.
26. Kawata Y, Si Yano, Kojima H, Toyomizu M (2004) Transformation of Spirulina platensis Strain. (Arthrospira SP PCC C1: 9438) with Tn5 Transposase-Transposon DNA-Cation Liposome Complex. Marine Biotechnology 6: 355-363.
27. Singh DP, Singh N, (1997) Isolation and characterization of a metronidazole tolerant mutant of the cyanobacterium Spirulina platensis exhibiting multiple stress tolerance. World J Microbiol Biotechnol 13: 179-183. doi:10.1023/A:1018537730287.
28. Riccardi G, Sora S, Ciferri O, (1981) PRODUCTION OF AMINO-ACIDS BY ANALOG-RESISTANT MUTANTS OF THE CYANOBACTERIUM SPIRULINA-PLATENSIS. J Bacteriol 147: 1002-1007. PubMed: 6792182.
29. Riccardi G, De Rossi E, Milano A, De Felice M, (1988) Mutants of Spirulina platensis resistant to valine inhibition. FEMS Microbiol Lett 49: 19-23. doi:10.1016/0378-1097(88)90095-X.
30. Lanfaloni L, Trinei M, Russo M, Gualerzi CO, (1991) Mutagenesis of the Cyanobacterium Spirulina-Platensis by Uv and Nitrosoguanidine Treatment. FEMS Microbiol Lett 83: 85-90. doi:10.1111/j.1574-6968.1991.tb04394.x.
31. Wang LY, Huang ZL, Li G, Zhao HX, Xing XH, et al. (2010) Novel mutation breeding method for Streptomyces avermitilis using an atmospheric pressure glow discharge plasma. J Appl Microbiol 108: 851-858. doi:10.1111/j.1365-2672.2009.04483.x. PubMed: 19735332.
32. He-Ping L, Zhi-Bin W, Nan G, Pei-Si L, Hao W, et al. (2012) Studies on the Physical Characteristics of the Radio-Frequency Atmospheric-Pressure Glow Discharge Plasmas for the Genome Mutation of Methylosinus trichosporium. Plasmas Science IEEE Transactions On 40: 2853-2860. doi:10.1109/TPS.2012.2213274.
33. Li G, Li H-P, Wang L-Y, Wang S, Zhao H-X, et al. (2008) Genetic effects of radio-frequency, atmospheric-pressure glow discharges with helium. Appl Phys Lett 92: 221504. doi:10.1063/1.2938692.
34. Li HP, Li G, Wang LY, Wang S, Zhao HX, et al. (2008) Genetic effects of radio-frequency, atmospheric-pressure glow discharges with helium. Appl Phys Lett 92.
35. Wang LY, Huang ZL, Li G, Zhao HX, Xing XH, et al. (2010) Novel mutation breeding method for Streptomyces avermitilis using an atmospheric pressure glow discharge plasma. J Appl Microbiol 108: 851-858. doi:10.1111/j.1365-2672.2009.04483.x. PubMed: 19735332.
36. Li H-P, Sun W-T, Wang H-B, Li G, Bao C-Y, (2007) Electrical Features of Radio-frequency, Atmospheric-pressure, Bare-metallic-electrode Glow Discharges. Plasma Chem Plasma Process 27: 529-545. doi:10.1007/s11090-007-9079-x.
37. C Z; (1966) Contribution a l'etude d' une cyanophycee. Influence de diverse facteurs physiques et chimiques sur la croissance et la photosynthese de Spirulina maxima (Setch et Gardner) Geitler [PhD thesis]. Paris: University of Paris. 4-5 p.
38. Miller GL, (1959) Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal Chem 31: 426-428. doi:10.1021/ac60147a030.
39. Lee Y-K, (2004) Handbook of Microalgal Culture: Biotechnology and Applied Phycology; Richmind A, eds. Ames, IA: Blackwell Publishing House Science Ltd.
40. Chen H-B, Wu J-Y, Wang C-F, Fu C-C, Shieh C-J, et al. (2010) Modeling on chlorophyll a and phycocyanin production by Spirulina platensis under various light-emitting diodes. Biochem Eng J 53: 52-56. doi:10.1016/j.bej.2010.09.004.
41. Jin L, Fang M, Zhang C, Jiang P, Ge N, et al. (2011) Operating conditions for the rapid mutation of the oleaginous yeast by atmospheric and room temperature plasmas and the characteristics of the mutants. Chin J Biotechnol 27: 461-467.
42. Lu Y, Wang L, Ma K, Li G, Zhang C, et al. (2011) Characteristics of hydrogen production of an Enterobacter aerogenes mutant generated by a new atmospheric and room temperature plasma (ARTP). Biochem Eng J 55: 17-22. doi:10.1016/j.bej.2011.02.020.
43. Hua X, Wang J, Wu Z, Zhang H, Li H, et al. (2010) A salt tolerant Enterobacter cloacae mutant for bioaugmentation of petroleum- and salt-contaminated soil. Biochem Eng J 49: 201-206. doi:10.1016/j.bej.2009.12.014.
44. Li H-P, Li G, Sun W-T, Wang S, Bao C-Y, et al. (2008) Radio-Frequency, Atmospheric-Pressure Glow Discharges: Producing Methods, Characteristics and Applications in Bio-Medical Fields. AIP Conf Proc 982: 584-591. doi:10.1063/1.2897862.
45. Li H-P, Wang L-Y, Li G, Jin L-H, Le P-S, et al. (2011) Manipulation of Lipase Activity by the Helium Radio-Frequency, Atmospheric-Pressure Glow Discharge Plasma Jet. Plasma Processes Polym 8: 224-229. doi:10.1002/ppap.201000035.
46. Gao K, Ma Z, (2008) Photosynthesis and growth of Arthrospira (Spirulina) platensis (Cyanophyta) in response to solar UV radiation, with special reference to its minor variant. Environ Exp Bot 63: 123-129. doi:10.1016/j.envexpbot.2007.10.031.
47. Wang ZP, Zhao Y, (2005) MORPHOLOGICAL REVERSION OF Spirulina (Arthrospira) PLATENSIS (CYANOPHYTA): FROM LINEAR TO HELICAL1. J Phycol 41: 622-628. doi:10.1111/j.1529-8817.2005.00087.x.
48. Wu H, Gao K, Ma Z, Watanabe T, (2005) Effects of solar ultraviolet radiation on biomass production and pigment contents of Spirulina platensis in commercial operations under sunny and cloudy weather conditions. Fish Sci 71: 454-456. doi:10.1111/j.1444-2906.2005.00984.x.