A high-throughput sample preparation method for cellular proteomics using 96-well filter plates

Proteomics

Volumn 13, Issue 20, 2013, Pages 2980-2983

  Switzar, Linda ^a   van Angeren, Jordy ^a   Pinkse, Martijn ^b   Kool, Jeroen ^a   Niessen, Wilfried M A ^a,c  

^a VU UNIVERSITY AMSTERDAM   (Netherlands)

^b DELFT UNIVERSITY OF TECHNOLOGY   (Netherlands)

^c Hyphen MassSpec   (Netherlands)

Author keywords

Bottom up proteomics; Cellular proteomics; Filter plate; High throughput sample preparation; LC MS; Technology

Indexed keywords

CELL PROTEIN; ORGANIC SOLVENT;

ALKYLATION; ANALYTIC METHOD; ARTICLE; AUTOMATION; CELL LYSATE; CELL MEMBRANE; CYTOLYSIS; FILTER; GEL FILTRATION; HUMAN; HUMAN CELL; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN DENATURATION; PROTEOMICS; REDUCTION;

BOTTOM-UP PROTEOMICS; CELLULAR PROTEOMICS; FILTER PLATE; HIGH-THROUGHPUT SAMPLE PREPARATION; LC-MS; TECHNOLOGY;

ANALYTIC SAMPLE PREPARATION METHODS; CELL EXTRACTS; CELLS; CHROMATOGRAPHY, LIQUID; HEK293 CELLS; HIGH-THROUGHPUT SCREENING ASSAYS; HUMANS; MASS SPECTROMETRY; NANOTECHNOLOGY; PEPTIDES; PROTEINS; PROTEOMICS;

EID: 84885400747     PISSN: 16159853     EISSN: 16159861     Source Type: Journal    
DOI: 10.1002/pmic.201300080     Document Type: Article

References (12)

1. Levin, Y., Schwarz, E., Wang, L., Leweke, F. M., Bahn, S., Label-free LC-MS/MS quantitative proteomics for large-scale biomarker discovery in complex samples. J. Sep. Sci. 2007, 30, 2198-2203.
2. Simpson, D. C., Ahn, S., Pasa-Tolic, L., Bogdanov, B. etal., Using size exclusion chromatography-RPLC and RPLC-CIEF as two-dimensional separation strategies for protein profiling. Electrophoresis 2006, 27, 2722-2733.
3. Gundry, R. L., White, M. Y., Murray, C. I., Kane, L. A. etal., Preparation of proteins and peptides for mass spectrometry analysis in a bottom-up proteomics workflow. Curr. Protoc. Mol. Biol. 2009, Chapter 10, Unit 10.25.
4. Guo, X., Kristal, B. S., The use of underloaded C(18) solid-phase extraction plates increases reproducibility of analysis of tryptic peptides from unfractionated human plasma. Anal. Biochem. 2012, 426, 86-90.
5. Switzar, L., Giera, M., Lingeman, H., Irth, H., Niessen, W. M., Protein digestion optimization for characterization of drug-protein adducts using response surface modeling. J. Chromatogr. A 2011, 1218, 1715-1723.
6. Wisniewski, J. R., Zougman, A., Nagaraj, N., Mann, M., Universal sample preparation method for proteome analysis. Nat. Methods 2009, 6, 359-362.
7. Katayama, H., Satoh, K., Takeuchi, M., Deguchi-Tawarada, M. etal., Optimization of in-gel protein digestion system in combination with thin-gel separation and negative staining in 96-well plate format. Rapid Commun. Mass Spectrom. 2003, 17, 1071-1078.
8. Pluskal, M. G., Bogdanova, A., Lopez, M., Gutierrez, S., Pitt, A. M., Multiwell in-gel protein digestion and microscale sample preparation for protein identification by mass spectrometry. Proteomics 2002, 2, 145-150.
9. Nissum, M., Schneider, U., Kuhfuss, S., Obermaier, C. etal., In-gel digestion of proteins using a solid-phase extraction microplate. Anal. Chem. 2004, 76, 2040-2045.
10. Chelius, D., Xiao, G., Nichols, A. C., Vizel, A. etal., Automated tryptic digestion procedure for HPLC/MS/MS peptide mapping of immunoglobulin gamma antibodies in pharmaceutics. J. Pharm. Biomed. Anal. 2008, 47, 285-294.
11. Hou, W., Ethier, M., Smith, J. C., Sheng, Y., Figeys, D., Multiplexed proteomic reactor for the processing of proteomic samples. Anal. Chem. 2007, 79, 39-44.
12. Batruch, I., Lecker, I., Kagedan, D., Smith, C. R. etal., Proteomic analysis of seminal plasma from normal volunteers and post-vasectomy patients identifies over 2000 proteins and candidate biomarkers of the urogenital system. J. Proteome Res. 2011, 10, 941-953.