Preparation of proteins and peptides for mass spectrometry analysis in a bottom-up proteomics workflow

Current Protocols in Molecular Biology

Volumn , Issue SUPPL. 88, 2009, Pages

  Gundry, Rebekah L ^a   White, Melanie Y ^a   Murray, Christopher I ^a   Kane, Lesley A ^a   Fu, Qin ^a   Stanley, Brian A ^a   Van Eyk, Jennifer E ^a  

^a JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

In gel digestion; In solution digestion; Mass spectrometry; Peptide desalting proteomics

Indexed keywords

ANAZOLENE SODIUM; PHOSPHOPEPTIDE; POLYACRYLAMIDE GEL; TITANIUM DIOXIDE; PEPTIDE; PROTEIN;

AFFINITY CHROMATOGRAPHY; ALGORITHM; ARTICLE; DISULFIDE BOND; ELECTROSPRAY MASS SPECTROMETRY; ENZYME ACTIVITY; GEL ELECTROPHORESIS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; ISOELECTRIC POINT; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DATABASE; PROTEIN PROCESSING; PROTEIN PURIFICATION; PROTEOMICS; REVERSED PHASE LIQUID CHROMATOGRAPHY; SEPARATION TECHNIQUE; TAXONOMY; WORKFLOW; AMINO ACID SEQUENCE; BINDING AFFINITY; ENZYMATIC DEGRADATION; PROTEIN DEGRADATION;

MASS SPECTROMETRY; PEPTIDES; PROTEINS; PROTEOMICS; SPECIMEN HANDLING;

EID: 78651366142     PISSN: 19343639     EISSN: 19343647     Source Type: Journal    
DOI: 10.1002/0471142727.mb1025s88     Document Type: Article

References (26)

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19. 2 are combined in a sequential manner to allow expanded phosphopeptide enrichment.
20. Shevchenko et al., 1996. See above. This is the most commonly used silver staining and peptide extraction method for MS compatible samples.
21. http://ca.expasy.org/ch2d/ Two-dimensional Polyacrylamide gel electrophoresis database.
22. http://ca.expasy.org/tools Database of proteomic analysis tools which include cleavage site predictions, modification predictions, topology prediction, and visualization software, among others.
23. http://www.ionsource.com/ Database of useful protocols for preparing proteomic samples, as well as tutorials and principles of chromatography and mass spectrometry.
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26. http://www.proteomecenter.org/course.php Excellent repository of lecture notes for proteomics, mass spectrometry, and bioinformatics courses designed for beginners, taught at the Institute for Systems Biology at the Seattle Proteome Center.