A method for purifying high quality and high yield plasmid DNA for metagenomic and deep sequencing approaches

Journal of Microbiological Methods

Volumn 95, Issue 2, 2013, Pages 272-279

  Brown Kav, Aya ^a,b   Benhar, Itai ^b   Mizrahi, Itzhak ^a  

^a VOLCANI CENTER   (Israel)

^b TEL AVIV UNIVERSITY   (Israel)

Author keywords

Horizontal gene transfer; Lateral gene transfer; Microbial ecology; Plasmid purification; Plasmidome; Rumen microbial community

Indexed keywords

PLASMID DNA;

ARTICLE; BACTERIAL GENE; BACTERIAL GENOME; CONTROLLED STUDY; DEEP SEQUENCING TECHNIQUE; DNA CONTAMINATION; DNA EXTRACTION; DNA PURIFICATION; DNA SEQUENCE; ECOLOGICAL NICHE; GENE AMPLIFICATION; GENE DOSAGE; GENE EXPRESSION; GENE FUNCTION; GENE INDUCTION; GENE STRUCTURE; GENETIC ANALYSIS; GENETIC HETEROGENEITY; GENETIC TRAIT; HETEROZYGOTE; METAGENOMICS; MICROBIAL COMMUNITY; MICROBIAL GENETICS; MICROORGANISM; MOLECULAR EVOLUTION; NONHUMAN; PLASMID; POPULATION ABUNDANCE; PRIORITY JOURNAL; QUALITY CONTROL; RUMEN MICROORGANISM; SUPERNUMERARY CHROMOSOME;

HORIZONTAL GENE TRANSFER; LATERAL GENE TRANSFER; MICROBIAL ECOLOGY; PLASMID PURIFICATION; PLASMIDOME; RUMEN MICROBIAL COMMUNITY;

ANIMALS; CATTLE; DNA, BACTERIAL; HIGH-THROUGHPUT NUCLEOTIDE SEQUENCING; METAGENOMICS; PLASMIDS; POLYMERASE CHAIN REACTION; RUMEN; SEQUENCE ANALYSIS, DNA;

EID: 84885220942     PISSN: 01677012     EISSN: 18728359     Source Type: Journal    
DOI: 10.1016/j.mimet.2013.09.008     Document Type: Article

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